| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
Peroxyfluor 1 targets H2O2 via a boronate-to-phenol oxidative conversion reaction. H2O2 oxidatively cleaves the boronate ester groups, yielding the highly fluorescent fluorescein product. It does not react with other reactive oxygen species (e.g., superoxide, peroxynitrite) appreciably.
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|---|---|
| ln Vitro |
The probe exhibits no significant fluorescence in its reduced (boronate) form. Upon reaction with H2O2 (Km ~ 13 uM), it undergoes rapid oxidation to fluorescein, producing a 100-fold fluorescence increase. Reactivity is selective for H2O2 over other ROS.
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| ln Vivo |
No in vivo activity data are documented as Peroxyfluor 1 is a fluorescent probe for cellular H2O2 detection, not a therapeutic agent for systemic administration. It is intended for in vitro live-cell imaging applications only.
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| Enzyme Assay |
This assay is performed in a cell-free system using purified H2O2 standards. Peroxyfluor 1 (1-10 uM) is incubated with varying concentrations of H2O2 (0-100 uM) in PBS buffer at 37degC for 30-60 min. Fluorescence is measured at Ex/Em 345/410 nm (or 488/520 nm after oxidation) to establish a standard curve.
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| Cell Assay |
Cells are loaded with 1-10 uM Peroxyfluor 1 in culture medium for 30-60 min at 37degC, followed by washing. H2O2 production is induced by stimuli (e.g., PMA). Intracellular fluorescence is monitored by confocal microscopy or flow cytometry at Ex/Em 488/520 nm to measure ROS levels.
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| Animal Protocol |
Peroxyfluor 1 is not for in vivo animal administration. For ex vivo tissue studies, fresh tissue sections are incubated with 5-10 uM probe for 30-60 min at 37degC, followed by washing and imaging to detect endogenous H2O2 production.
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| ADME/Pharmacokinetics |
The probe is stable as a powder at -20degC for 3 years and as a solution at -80degC for 1 year. DMSO solubility: 14.29 mg/mL (25.88 mM) with pH adjustment to 10 using 1M NaOH. Ex/Em: 345/410 nm (boronate form) and 488/520 nm (fluorescein form).
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| Toxicity/Toxicokinetics |
Standard laboratory safety precautions should be followed. Peroxyfluor 1 is for research use only. Avoid inhalation, ingestion, and skin contact. Use personal protective equipment and work in a well-ventilated area.
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| References | |
| Additional Infomation |
This compound is a research-use-only fluorescent probe for H2O2 detection in oxidative stress and cell signaling studies, not an approved drug. Molecular formula: C32H34B2O7; molecular weight: 552.23. It is highly sensitive and selective for H2O2 over other ROS species.
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| Molecular Formula |
C32H34B2O7
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|---|---|
| Molecular Weight |
552.23000
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| Exact Mass |
552.249
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| CAS # |
817170-66-4
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| PubChem CID |
15983937
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| Appearance |
White to off-white solid powder
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| LogP |
4.853
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
41
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| Complexity |
967
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| Defined Atom Stereocenter Count |
0
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| SMILES |
B1(OC(C(O1)(C)C)(C)C)C2=CC3=C(C=C2)C4(C5=C(O3)C=C(C=C5)B6OC(C(O6)(C)C)(C)C)C7=CC=CC=C7C(=O)O4
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| InChi Key |
VXEWNNJWGHKNQG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C32H34B2O7/c1-28(2)29(3,4)39-33(38-28)19-13-15-23-25(17-19)36-26-18-20(34-40-30(5,6)31(7,8)41-34)14-16-24(26)32(23)22-12-10-9-11-21(22)27(35)37-32/h9-18H,1-8H3
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| Chemical Name |
3',6'-bis(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~14.29 mg/mL (~25.88 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8108 mL | 9.0542 mL | 18.1084 mL | |
| 5 mM | 0.3622 mL | 1.8108 mL | 3.6217 mL | |
| 10 mM | 0.1811 mL | 0.9054 mL | 1.8108 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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