| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
PC Biotin-PEG3-alkyne does not have a specific biological target. It is a cleavable PEG linker used in the synthesis of antibody-drug conjugates (ADCs) and bioconjugates. The compound contains biotin, which allows for affinity purification and detection using streptavidin-based methods. The alkyne group enables click chemistry conjugation to azide-containing molecules. The PEG spacer increases solubility in aqueous media. The cleavable moiety allows for controlled release of the payload in the target cells. PC Biotin-PEG3-alkyne is used in the development of ADCs and other targeted therapies.
|
|---|---|
| ln Vitro |
ADC cytotoxins are connected to antibodies through an ADC connector to form ADCs [1].
PC Biotin-PEG3-alkyne is not evaluated for direct biological activity in cell-based assays. It is a linker molecule used to improve the physicochemical properties of drug molecules and bioconjugates. The compound's utility is assessed by its ability to facilitate conjugation reactions and improve the solubility, stability, and pharmacokinetics of the conjugated molecules. PC Biotin-PEG3-alkyne is used in the synthesis of ADCs and other bioconjugates. The compound's cleavable nature allows for controlled release of the payload, which is essential for the efficacy of ADCs. |
| ln Vivo |
PC Biotin-PEG3-alkyne is not administered as a therapeutic agent. It is used as a linker in the synthesis of antibody-drug conjugates (ADCs) and other bioconjugates. The in vivo effects of PC Biotin-PEG3-alkyne are related to its ability to improve the properties of the conjugated molecules. The cleavable linker allows for controlled release of the payload in the target cells, enhancing the efficacy and safety of the ADC. The PEG spacer increases solubility and reduces immunogenicity of the conjugate.
|
| Enzyme Assay |
PC Biotin-PEG3-alkyne does not have established receptor binding or enzyme inhibition assay protocols. Its properties are characterized by standard analytical methods including NMR, HPLC, and mass spectrometry. Physical properties: molecular weight 780.88 g/mol; molecular formula C35H52N6O12S; appearance: solid; purity: typically ≥95%; solubility: soluble in DMSO and organic solvents. The compound's click chemistry reactivity is assessed by monitoring the CuAAc reaction with azide-containing molecules.
|
| Cell Assay |
Cellular studies with PC Biotin-PEG3-alkyne are not typically conducted as it is a linker molecule rather than a pharmacologically active agent. Its effects are assessed indirectly through the properties of the conjugated molecules. If used in cell-based research, it would be as a component of drug conjugates or as a control compound. Standard cell culture protocols would apply if cellular effects were to be evaluated.
|
| Animal Protocol |
In vivo studies are not conducted with PC Biotin-PEG3-alkyne as a standalone compound. It is used as a linker in the synthesis of ADCs and other bioconjugates. The in vivo effects of PC Biotin-PEG3-alkyne are assessed indirectly through the properties of the conjugated molecules.
|
| ADME/Pharmacokinetics |
Molecular Weight: 780.88. Formula: C35H52N6O12S. CAS No.: 1869922-24-6. Synonyms: PC Biotin-PEG3-alkyne. Appearance: Solid. Purity: Typically ≥95%. Solubility: Soluble in DMSO. Storage: Typically at -20°C. PC Biotin-PEG3-alkyne is a cleavable, 3-unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs). It contains biotin for affinity purification and an alkyne group for click chemistry.
|
| Toxicity/Toxicokinetics |
No toxicology data are available for PC Biotin-PEG3-alkyne as it is a linker molecule rather than a pharmaceutical compound. The compound is intended for laboratory use only and is not intended for human consumption or therapeutic use. Standard safety precautions for handling chemicals should be followed.
|
| References |
[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337.
|
| Additional Infomation |
PC Biotin-PEG3-alkyne is also known as PC Biotin-PEG3-alkyne (cleavable ADC linker). Its IUPAC name is not fully resolved in publicly available sources. PC Biotin-PEG3-alkyne is a cleavable, 3-unit PEG linker used in the synthesis of antibody-drug conjugates (ADCs). It contains biotin for affinity purification and an alkyne group for click chemistry (CuAAc). The compound is used in bioconjugation applications and in the development of targeted therapies. No clinical trials have been reported for this compound. PC Biotin-PEG3-alkyne is for research use only.
|
| Molecular Formula |
C35H52N6O12S
|
|---|---|
| Molecular Weight |
780.885388374329
|
| Exact Mass |
780.336
|
| CAS # |
1869922-24-6
|
| PubChem CID |
91757783
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
0.7
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
13
|
| Rotatable Bond Count |
27
|
| Heavy Atom Count |
54
|
| Complexity |
1230
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
CC(C1=CC(=C(C=C1[N+](=O)[O-])OCCCC(=O)NCCOCCOCCOCCNC(=O)CCCC[C@H]2[C@@H]3[C@H](CS2)NC(=O)N3)OC)OC(=O)NCC#C
|
| InChi Key |
HRHZIAGPIYYJMG-MJUSKHNJSA-N
|
| InChi Code |
InChI=1S/C35H52N6O12S/c1-4-11-38-35(45)53-24(2)25-21-28(48-3)29(22-27(25)41(46)47)52-14-7-10-32(43)37-13-16-50-18-20-51-19-17-49-15-12-36-31(42)9-6-5-8-30-33-26(23-54-30)39-34(44)40-33/h1,21-22,24,26,30,33H,5-20,23H2,2-3H3,(H,36,42)(H,37,43)(H,38,45)(H2,39,40,44)/t24?,26-,30-,33-/m0/s1
|
| Chemical Name |
1-[4-[4-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethylamino]-4-oxobutoxy]-5-methoxy-2-nitrophenyl]ethyl N-prop-2-ynylcarbamate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2806 mL | 6.4030 mL | 12.8059 mL | |
| 5 mM | 0.2561 mL | 1.2806 mL | 2.5612 mL | |
| 10 mM | 0.1281 mL | 0.6403 mL | 1.2806 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
