| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
The primary targets of Palmitoyl Tetrapeptide-3 are believed to be receptors or signaling pathways involved in the immune response and extracellular matrix dynamics. It has phagocytosis-stimulating activity, suggesting it can activate immune cells. It is also an interleukin-6 (IL-6) inhibitor, targeting inflammatory pathways in skin cells. It stimulates collagen production and promotes skin firmness and elasticity.
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|---|---|
| ln Vitro |
In vitro, Palmitoyl Tetrapeptide-3 stimulates phagocytosis. It acts as an IL-6 inhibitor, targeting inflammatory pathways. It is believed to stimulate collagen production and promote skin firmness and elasticity. These activities make it a popular ingredient in cosmetic and dermatological research.
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| ln Vivo |
In vivo, Palmitoyl Tetrapeptide-3 is used in cosmetic products for its anti-aging effects. It is believed to reduce the appearance of wrinkles and fine lines by enhancing collagen production. Its activity as an IL-6 inhibitor may also help reduce skin inflammation. However, its primary use is in topical formulations for skin care.
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| Enzyme Assay |
For phagocytosis assays, immune cells (e.g., macrophages) are cultured and incubated with Palmitoyl Tetrapeptide-3. Fluorescently labeled particles (e.g., bacteria or beads) are added, and the uptake of particles by cells is measured by flow cytometry or fluorescence microscopy. For IL-6 inhibition, cells are stimulated with LPS and cytokine levels are measured by ELISA.
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| Cell Assay |
For cellular studies, skin cells (e.g., fibroblasts or keratinocytes) are cultured in appropriate medium. Palmitoyl Tetrapeptide-3 is dissolved in DMSO or water and diluted in culture medium. Cells are treated with the peptide. Collagen production is measured by ELISA or Western blot. Inflammatory cytokine production is measured by ELISA. Cell proliferation is assessed by MTT assay.
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| Animal Protocol |
For in vivo efficacy studies, animal models of skin aging or wound healing could be used. Palmitoyl Tetrapeptide-3 would be formulated in a topical cream and applied to the skin. Skin thickness, collagen content, and wrinkle formation would be assessed. Inflammatory markers would be measured in skin biopsies.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Palmitoyl Tetrapeptide-3 are not applicable for topical use. As a peptide, it is unlikely to be systemically absorbed when applied topically. Its effects are expected to be local. For systemic administration, it would likely be rapidly degraded by proteases.
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| Toxicity/Toxicokinetics |
Toxicological data for Palmitoyl Tetrapeptide-3 are limited, but it is generally considered safe for topical use in cosmetics. No significant toxicity has been reported. As with all research chemicals, appropriate safety precautions should be taken during handling.
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| Additional Infomation |
See also: ...View more...
Palmitoyl Tetrapeptide-3 (CAS 221227-05-0) is a synthetic peptide with phagocytosis-stimulating activity. It has the sequence Palm-GQPR and a molecular weight of 694.91. It is an IL-6 inhibitor and stimulates collagen production. It is used in skin care research and is strictly for research use. |
| Molecular Formula |
C34H62N8O7
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|---|---|
| Molecular Weight |
694.9055
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| Exact Mass |
694.474
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| CAS # |
221227-05-0
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| PubChem CID |
10078408
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Index of Refraction |
1.585
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| LogP |
4.48
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
28
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| Heavy Atom Count |
49
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| Complexity |
1070
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O=C([C@]([H])(C([H])([H])C([H])([H])C(N([H])[H])=O)N([H])C(C([H])([H])N([H])C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])=O)=O)N1C([H])([H])C([H])([H])C([H])([H])[C@@]1([H])C(N([H])[C@]([H])(C(=O)O[H])C([H])([H])C([H])([H])C([H])([H])/N=C(\N([H])[H])/N([H])[H])=O
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| InChi Key |
IHRKJQSLKLYWBQ-QKDODKLFSA-N
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| InChi Code |
InChI=1S/C34H62N8O7/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-19-29(44)39-24-30(45)40-25(20-21-28(35)43)32(47)42-23-16-18-27(42)31(46)41-26(33(48)49)17-15-22-38-34(36)37/h25-27H,2-24H2,1H3,(H2,35,43)(H,39,44)(H,40,45)(H,41,46)(H,48,49)(H4,36,37,38)/t25-,26-,27-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-1-[(2S)-5-amino-2-[[2-(hexadecanoylamino)acetyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF : 10 mg/mL (~14.39 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4390 mL | 7.1952 mL | 14.3904 mL | |
| 5 mM | 0.2878 mL | 1.4390 mL | 2.8781 mL | |
| 10 mM | 0.1439 mL | 0.7195 mL | 1.4390 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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