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| Other Sizes |
| Targets |
Oil Red O does not have a specific biological target as a drug. It is a lipophilic dye used for staining lipids in histological and cytological specimens. The dye is a strong lipid solvent and staining agent that can specifically stain neutral triglycerides, lipids, and lipoproteins in tissues and cells. When tissue sections are placed in the dye solution, the dye leaves the solution and dissolves into the lipids within the tissue, staining lipid droplets red.
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| ln Vitro |
Oil Red O does not have direct in vitro biological activity as a pharmacological agent. Its value is as a histological stain for the detection of lipids in biological specimens. It is used to stain neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It is also used to analyze muscle fibers.
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| ln Vivo |
Oil Red O does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for histological staining.
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| Enzyme Assay |
In vitro staining protocols for Oil Red O involve fixing the specimen (e.g., tissue section, cell culture) and incubating with a saturated solution of Oil Red O in isopropanol or propylene glycol. The specimen is then washed and counterstained if necessary. Lipids appear red under light microscopy.
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| Cell Assay |
Cell-based assays using Oil Red O are conducted to detect and quantify lipid accumulation in cultured cells. Cells are fixed and stained with Oil Red O. The stained lipid droplets can be visualized by light microscopy or quantified by extracting the dye and measuring absorbance.
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| Animal Protocol |
In vivo animal experiments using Oil Red O are conducted to assess lipid accumulation in tissues. Liver biopsies from mice are stained with Oil Red O to detect and quantify hepatic steatosis. The dye is used to stain neutral triglycerides and lipids on frozen sections.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Oil Red O as it is a histological stain, not a therapeutic agent. The compound has a molecular formula of C26H24N4O and a molecular weight of 408.50. Melting point: 120 °C. Appearance: Brownish red powder. Solubility: Insoluble in water. Storage: room temperature.
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| Toxicity/Toxicokinetics |
Safety and toxicology data for Oil Red O are limited. As a histological stain, it should be handled with appropriate safety precautions. The compound is for research and diagnostic use only and is not for human therapeutic use. No specific toxicity data are available.
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| References | |
| Additional Infomation |
Oil Red O is a diazo compound, a 2-naphthol compound in which 2-naphthol is substituted at the 1-position with a {4-[(2,5-dimethylphenyl)diazo]-2,5-dimethylphenyl}diazo group. It is a lipid-soluble dye primarily used for staining triglycerides in frozen sections and for staining protein-bound lipids in paraffin sections. It is a histological dye. Oil Red O belongs to the azobenzene, diazo, and naphthol classes, and its function is similar to that of 2-naphthol.
Oil Red O has CAS number 1320-06-5, molecular formula C26H24N4O, and molecular weight 408.50. Synonyms: Solvent Red 27. It is a fat-soluble diazo dye used for staining neutral lipids and cholesteryl esters. Max absorption: 518 nm. Not for human use; for research and diagnostic applications. |
| Molecular Formula |
C26H24N4O
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|---|---|
| Molecular Weight |
408.4950
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| Exact Mass |
408.195
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| CAS # |
1320-06-5
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| PubChem CID |
2724054
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| Appearance |
Brown to black solid
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
638.8±55.0 °C at 760 mmHg
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| Melting Point |
120ºC
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| Flash Point |
439.7±20.8 °C
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| Vapour Pressure |
0.0±2.0 mmHg at 25°C
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| Index of Refraction |
1.631
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| LogP |
7.58
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
31
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| Complexity |
637
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O([H])C1C([H])=C([H])C2=C([H])C([H])=C([H])C([H])=C2C=1/N=N/C1=C([H])C(C([H])([H])[H])=C(C([H])=C1C([H])([H])[H])/N=N/C1C([H])=C(C([H])([H])[H])C([H])=C([H])C=1C([H])([H])[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~4.17 mg/mL (~10.21 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4480 mL | 12.2399 mL | 24.4798 mL | |
| 5 mM | 0.4896 mL | 2.4480 mL | 4.8960 mL | |
| 10 mM | 0.2448 mL | 1.2240 mL | 2.4480 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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