| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Octadecyl Rhodamine B chloride targets cell membranes, binding to lipid bilayers through its lipophilic octadecyl chain. The compound is a cationic amphiphile that intercalates into cellular membranes, making it useful for studying membrane structure, lipid bilayer dynamics, and membrane protein distribution. It can be used to stain live cells and to examine membrane integrity and cellular uptake processes. The compound's fluorescent properties allow for visualization by fluorescence microscopy.
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| ln Vitro |
Octadecyl Rhodamine B chloride is a lipophilic fluorescent dye used for cell membrane staining. The compound binds to cellular membranes and can be used to stain live cells. It exhibits fluorescence properties typical of rhodamine dyes, with large absorption and luminescence, excellent light resistance, and low toxicity in vivo. The compound is an amphiphilic fluorescent probe that easily forms aggregates in aqueous phase, leading to self-quenching. It can be used in studies of membrane structures, protein domains, and electron energy transfer in organic molecular assemblies.
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| ln Vivo |
In vivo activity data for Octadecyl Rhodamine B chloride are primarily related to its use as a fluorescent probe for imaging applications. The compound has low toxicity in vivo and can be used for cell membrane staining in living organisms. However, specific in vivo efficacy studies are not applicable, as the compound is used as a research tool rather than a therapeutic agent. The compound's membrane-binding properties make it useful for studying membrane dynamics in vivo.
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| Enzyme Assay |
In vitro assays for Octadecyl Rhodamine B chloride typically involve staining cells or membrane preparations with the compound. Cells are incubated with the dye at concentrations typically ranging from 0.1 to 10 μg/mL for 5-30 minutes at 37°C, followed by washing to remove unbound dye. Stained cells are then analyzed by fluorescence microscopy or flow cytometry. The compound's membrane-binding properties can be assessed by measuring fluorescence intensity associated with cellular membranes. The compound is dissolved in DMSO or ethanol as a stock solution and diluted in aqueous buffer.
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| Cell Assay |
In vitro cellular assays for Octadecyl Rhodamine B chloride typically involve treating cultured cells with the compound at concentrations ranging from 0.1 to 10 μg/mL for 5-30 minutes. Stained cells are then analyzed by fluorescence microscopy to visualize membrane structures. Membrane integrity can be assessed by examining the distribution and intensity of fluorescence. Cellular uptake and membrane dynamics can be studied using time-lapse imaging. The compound is dissolved in DMSO as a stock solution and diluted in cell culture medium.
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| Animal Protocol |
In vivo animal studies for Octadecyl Rhodamine B chloride typically involve administering the compound to animals via intravenous injection for imaging applications. The compound's membrane-binding properties allow for visualization of cell membranes in tissues. Tissue sections can be examined by fluorescence microscopy to study membrane structures. The compound has low toxicity in vivo, making it suitable for imaging studies. However, specific protocols for in vivo studies are not extensively documented.
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| ADME/Pharmacokinetics |
Octadecyl Rhodamine B chloride has a molecular weight of 731.50 g/mol and formula C46H67ClN2O3. The compound is a solid at room temperature and should be stored in a cool, dark place at temperatures below 15°C. The compound is soluble in organic solvents such as DMSO and ethanol. Detailed PK parameters are not applicable for this fluorescent probe, as it is primarily used for in vitro and imaging applications rather than as a therapeutic agent.
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| Toxicity/Toxicokinetics |
Toxicity data for Octadecyl Rhodamine B chloride indicate that it has low toxicity in vivo. The compound is intended for research use only and is not approved for human therapeutic applications. As with all chemical compounds, appropriate safety precautions should be taken when handling. Standard toxicity assessments would include acute toxicity, genotoxicity, and repeated-dose toxicity studies. The compound's cationic amphiphilic nature may contribute to membrane effects at high concentrations.
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| References | |
| Additional Infomation |
Octadecyl Rhodamine B chloride (CAS 65603-19-2) is a cationic amphiphilic fluorescent probe with membrane-binding ability. It has a molecular weight of 731.50 g/mol and formula C46H67ClN2O3. The compound is used for cell membrane staining and can be applied in studies of membrane structure, lipid bilayers, and cellular uptake processes. It has low toxicity in vivo and exhibits excellent light resistance. The compound is not approved for clinical use.
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| Molecular Formula |
C46H67N2O3+.CL-
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|---|---|
| Molecular Weight |
731.48878
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| Exact Mass |
730.484
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| CAS # |
65603-19-2
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| PubChem CID |
65232
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| Appearance |
Pale purple to purple solid-liquid Mixture
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| LogP |
9.285
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
25
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| Heavy Atom Count |
52
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| Complexity |
1090
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCCCCCOC(=O)c1ccccc1c2c3ccc(cc3oc-4cc(=[N+](CC)CC)ccc24)N(CC)CC.[Cl-]CopyCopied
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| InChi Key |
NFGODEMQGQNUKK-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C46H67N2O3.ClH/c1-6-11-12-13-14-15-16-17-18-19-20-21-22-23-24-27-34-50-46(49)40-29-26-25-28-39(40)45-41-32-30-37(47(7-2)8-3)35-43(41)51-44-36-38(31-33-42(44)45)48(9-4)10-5;/h25-26,28-33,35-36H,6-24,27,34H2,1-5H3;1H/q+1;/p-1
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| Chemical Name |
[6-(diethylamino)-9-(2-octadecoxycarbonylphenyl)xanthen-3-ylidene]-diethylazanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~136.71 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3671 mL | 6.8354 mL | 13.6707 mL | |
| 5 mM | 0.2734 mL | 1.3671 mL | 2.7341 mL | |
| 10 mM | 0.1367 mL | 0.6835 mL | 1.3671 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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