| Size | Price | Stock | Qty |
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| 1g |
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| 5g |
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| Other Sizes |
| Targets |
Nuclear Fast Red acts as a calcium indicator, forming bright red lakes with calcium and serving as a red nuclear staining lake when combined with aluminum. It binds to nucleic acids and nuclear components for contrast.
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| ln Vitro |
As a histological dye, Nuclear Fast Red provides contrast for nuclear visualization when used with other stains such as Prussian blue, hematoxylin, and eosin (H&E). It is an acidic anthraquinone dye used for staining cell nuclei in various tissue preparations.
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| ln Vivo |
No in vivo activity data are available as Nuclear Fast Red is a histological dye for ex vivo tissue staining, not used for systemic in vivo administration.
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| Enzyme Assay |
The general procedure involves preparing a 0.1% dye solution in 5% aluminum sulfate solution. Tissues are first stained with another dye, then immersed in the Nuclear Fast Red solution for 1-5 minutes at room temperature, followed by differentiation and mounting.
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| Cell Assay |
Cells are fixed onto slides, stained with the primary stain (e.g., Prussian blue), then incubated with 0.1% Nuclear Fast Red solution in 5% aluminum sulfate for 1-5 minutes. Slides are washed, dehydrated, cleared, and mounted for microscopic observation.
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| Animal Protocol |
Nuclear Fast Red is used for ex vivo histological analysis only, not for in vivo animal administration. Tissue sections are prepared and stained outside the living organism for microscopic examination.
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| ADME/Pharmacokinetics |
Nuclear Fast Red is stable when stored as a dry powder at room temperature. The staining solution (0.1% in 5% aluminum sulfate) is stable for several months at room temperature when protected from light.
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| Toxicity/Toxicokinetics |
Limited toxicity data are available for this reagent-grade dye. Standard laboratory safety protocols should be followed, including the use of gloves, eye protection, and appropriate ventilation when handling powder and solutions.
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| References | |
| Additional Infomation |
Nucleotide red is an organosodium salt, a monosodium salt of 4-amino-1,3-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid. It forms a bright red lake with calcium and a commonly used red nuclear staining lake with aluminum. It can be used as a histological and fluorescent dye. It contains nucleotide red (1-).
See also: Neutral Red (note moved to). This compound is a histological staining reagent exclusively for ex vivo use, not approved as a therapeutic drug. It is commonly used as a counterstain for Prussian blue in iron staining and in LacZ tissue analysis. Molecular formula: C14H8NNaO7S; molecular weight: 357.27. |
| Molecular Formula |
C14H8NNAO7S
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|---|---|
| Molecular Weight |
357.2706
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| Exact Mass |
356.991
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| CAS # |
6409-77-4
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| PubChem CID |
23677995
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| Appearance |
Brown to black solid powder
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| Melting Point |
300 °C
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| LogP |
2.021
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
24
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| Complexity |
634
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C1C(=C(C2C(C3=C([H])C([H])=C([H])C([H])=C3C(C=2C=1[O-])=O)=O)N([H])[H])O[H])(=O)(=O)O[H].[Na+]
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| InChi Key |
IFSXZLJQEKGQAF-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
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| Chemical Name |
sodium;4-amino-1,3-dihydroxy-9,10-dioxoanthracene-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7990 mL | 13.9950 mL | 27.9900 mL | |
| 5 mM | 0.5598 mL | 2.7990 mL | 5.5980 mL | |
| 10 mM | 0.2799 mL | 1.3995 mL | 2.7990 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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