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| ln Vitro |
Norartocarpetin exhibits a strong inhibitory effect on mushroom tyrosinase, with an IC50 value of 0.47 μM[1]. On NCI-H460 cells and MRC-9 cells, norartocarpetin (0-100 μM; 48 h) exhibits a dose-dependent cytotoxic effect with IC50 values of 22 μM and 85 μM, respectively [2]. The Ras/Raf/MAPK signaling pathway is blocked by norartocarpetin (0, 11, 22, and 44 μM; 24 hours) [2]. Human lung cancer cells (NCI-H460) undergo apoptosis when exposed to norartocarpetin (0, 11, 22, and 44 μM) for a 24-hour period [2]. S-phase cell cycle arrest is brought on by norartocarpetin (0, 11, 22, and 44 μM) [2]. Cell invasion is dose-dependently inhibited by norartocarpetin (22 μM; 24 hours) [2]. Cell migration is significantly inhibited by norartocarpetin (0, 11, 22, and 44 μM, 24 hours) [2].
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| Cell Assay |
Cell Viability Assay[2]
Cell Types: MRC-9 (normal fibroblasts) and NCI-H460 (human lung cancer cells) Tested Concentrations: 0, 3.12, 6.25, 12.5, 25, 50 and 100 μM (dissolve in DMSO first) 1.0 mg/mL, then diluted to different concentrations using DMSO, 30 μL, 20 minutes) Incubation Duration: 48 hrs (hours) Experimental Results: Inhibited the viability of NCI-H460 cells in a dose-dependent manner. Western Blot Analysis[2] Cell Types: MRC-9 and NCI-H460 Cell Tested Concentrations: 0, 11, 22 and 44 μM Incubation Duration: 24 hrs (hours) Experimental Results: Dramatically diminished expression of p-RAS, p-RAF and p-P38 and expression of RAS, RAF, and P38 did not change. Apoptosis analysis [2] Cell Types: MRC-9 and NCI-H460 Cell Tested Concentrations: 0. 11, 22 and 44 μM Incubation Duration: 24 h Experimental Results: Caused dose-dependent apoptosis and induced DNA damage. Cell cycle analysis[2] Cell Types: MRC-9 and NCI-H460 Cell Tested Concentrations: 0, 11, 22 and 44 μM Incubation Duration: Experimental Results: Shows increase in S phase cells and percentage of NCI-H460 LC cells in S phase at 0, |
| References |
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| Additional Infomation |
Norartocarpetin is a member of flavones.
Norartocarpetin has been reported in Morus lhou, Artocarpus integer, and other organisms with data available. |
| Molecular Formula |
C15H10O6
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|---|---|
| Molecular Weight |
286.236
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| Exact Mass |
286.048
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| CAS # |
520-30-9
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| PubChem CID |
5481970
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| Appearance |
White to light yellow solid powder
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| Melting Point |
332 - 335 °C
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| LogP |
2.282
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
447
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZSYPIPFQOQGYHH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H10O6/c16-7-1-2-9(10(18)3-7)13-6-12(20)15-11(19)4-8(17)5-14(15)21-13/h1-6,16-19H
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| Chemical Name |
2-(2,4-dihydroxyphenyl)-5,7-dihydroxychromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~349.36 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4936 mL | 17.4679 mL | 34.9357 mL | |
| 5 mM | 0.6987 mL | 3.4936 mL | 6.9871 mL | |
| 10 mM | 0.3494 mL | 1.7468 mL | 3.4936 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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