| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
As a labeled fatty acid, Nonanoic acid-d17 does not have a specific pharmacological target. Nonanoic acid is a medium-chain saturated fatty acid involved in energy metabolism and lipid biosynthesis. The deuterated form is used to study fatty acid metabolism, absorption, and distribution in various biological systems.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Nonanoic acid-d17 is used in vitro as a tracer to study fatty acid metabolism and lipid dynamics. The compound is added to cell culture media to track fatty acid uptake, incorporation into lipids, and metabolic fate. It does not exhibit intrinsic pharmacological activity but serves as a quantitative tool for studying cellular lipid metabolism. |
| ln Vivo |
In vivo, Nonanoic acid-d17 is used in metabolic studies to trace nonanoic acid absorption, distribution, metabolism, and excretion. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry.
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| Enzyme Assay |
As an analytical standard, Nonanoic acid-d17 is used in lipidomics and fatty acid analysis. Typical protocols involve spiking the labeled compound into samples prior to extraction and analysis by GC-MS or LC-MS. The compound serves as an internal standard to correct for matrix effects and instrument variability.
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| Cell Assay |
In vitro cell culture experiments using Nonanoic acid-d17 involve treating cells with the labeled fatty acid and analyzing its uptake and metabolism by mass spectrometry. The compound can be used to study fatty acid metabolism, beta-oxidation, and lipid synthesis in various cell types.
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| Animal Protocol |
In vivo animal studies using Nonanoic acid-d17 involve administering the compound to rodents via oral gavage. Blood samples are collected at various time points, and the concentration of the labeled compound is measured by mass spectrometry. Tissue distribution and metabolic profiling can also be performed.
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| ADME/Pharmacokinetics |
Nonanoic acid-d17 exhibits pharmacokinetic properties similar to those of unlabeled nonanoic acid. As a medium-chain fatty acid, it is absorbed from the gastrointestinal tract and transported via the portal vein to the liver, where it is metabolized via beta-oxidation. The deuterium label provides a distinct mass shift for analytical detection.
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| Toxicity/Toxicokinetics |
Nonanoic acid-d17 is considered safe for research use at typical concentrations. Nonanoic acid is a naturally occurring fatty acid with a well-established safety profile. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References |
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| Additional Infomation |
Nonanoic acid-d17 has a molecular formula of C9HD17O2 and a molecular weight of 175.30. Isotopic enrichment is typically 98 atom% D. The compound is primarily used as an internal standard for mass spectrometry-based quantification of nonanoic acid in lipidomics and metabolic studies.
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| Molecular Formula |
C9HD17O2
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|---|---|
| Molecular Weight |
175.34274
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| Exact Mass |
175.237
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| CAS # |
130348-94-6
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| Related CAS # |
Nonanoic acid;112-05-0
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| PubChem CID |
72735671
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| Appearance |
Colorless to light yellow liquid
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| LogP |
2.821
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
11
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| Complexity |
99.7
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC(C(C(C(C(C(C(C(C([2H])([2H])[2H])([2H])[2H])([2H])[2H])([2H])[2H])([2H])[2H])([2H])[2H])([2H])[2H])([2H])[2H])=O
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| InChi Key |
FBUKVWPVBMHYJY-OISRNESJSA-N
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| InChi Code |
InChI=1S/C9H18O2/c1-2-3-4-5-6-7-8-9(10)11/h2-8H2,1H3,(H,10,11)/i1D3,2D2,3D2,4D2,5D2,6D2,7D2,8D2
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| Chemical Name |
2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,9-heptadecadeuteriononanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~570.3 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (28.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (28.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 5 mg/mL (28.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7032 mL | 28.5160 mL | 57.0321 mL | |
| 5 mM | 1.1406 mL | 5.7032 mL | 11.4064 mL | |
| 10 mM | 0.5703 mL | 2.8516 mL | 5.7032 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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