| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 10g |
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| Other Sizes |
| Targets |
Parasitic protozoa, specifically Eimeria species that cause coccidiosis. Nitromide is an anticoccidial agent that inhibits the growth and replication of Eimeria species. As a nitrobenzamide, its mechanism of action may involve the disruption of essential metabolic processes in the parasite.
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| ln Vitro |
Nitromide (3,5-Dinitrobenzamide) is an anti-parasitic drug. It exhibits antimicrobial properties effective against protozoal infections and some bacterial pathogens. It has been used as an anticoccidial agent in poultry, inhibiting the growth and replication of Eimeria species, and as an antibacterial agent for Salmonella pullorum infections.
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| ln Vivo |
In vivo, Nitromide is effective as a feed additive for the prevention and control of coccidiosis in poultry. It has also been used to prevent and treat Salmonella pullorum infections in chickens and turkeys. Its in vivo efficacy is based on its ability to inhibit the growth of these pathogens in the gastrointestinal tract.
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| Enzyme Assay |
In vitro assays for Nitromide involve testing its antimicrobial activity against relevant pathogens. The minimum inhibitory concentration (MIC) can be determined using broth dilution or agar dilution methods against Eimeria species or Salmonella strains. The compound's ability to inhibit parasite growth or bacterial replication can be assessed in these assays.
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| Cell Assay |
In vitro cell-based assays for Nitromide are not typically performed, as its primary targets are parasites and bacteria. However, its cytotoxicity against host cells could be assessed in cell culture to evaluate its safety profile. Such studies are not detailed in the provided sources.
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| Animal Protocol |
In vivo animal studies for Nitromide are typically conducted in poultry. The compound is administered as a feed additive, and its efficacy in preventing or treating coccidiosis or Salmonella infections is assessed. Parameters such as mortality, weight gain, and lesion scores are measured.
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| ADME/Pharmacokinetics |
Nitromide has a molecular weight of 211.13 and a molecular formula of C7H5N3O5. It should be stored as a powder at -20°C for up to 3 years or in solution at -80°C for up to 2 years. Its solubility and other physicochemical properties are not detailed in the provided sources.
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| Toxicity/Toxicokinetics |
No specific toxicity data are documented for Nitromide in the provided sources. As a veterinary drug, its safety profile has been evaluated for use in poultry. It is intended for research use only and is not approved for human therapeutic applications. Standard laboratory safety practices should be followed when handling this compound.
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| Additional Infomation |
Nitromide is also known as 3,5-dinitrobenzamide and 硝米特. It is a synthetic nitrobenzamide anticoccidial agent. It has been used historically as a feed additive in poultry. Its CAS name is 3,5-Dinitrobenzamide. It is an anti-parasitic and antimicrobial agent.
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| Molecular Formula |
C7H5N3O5
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|---|---|
| Molecular Weight |
211.1317
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| Exact Mass |
211.023
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| CAS # |
121-81-3
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| PubChem CID |
4511
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.601g/cm3
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| Boiling Point |
314.5ºC at 760mmHg
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| Melting Point |
183-185 °C(lit.)
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| Flash Point |
144ºC
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| Vapour Pressure |
0.000466mmHg at 25°C
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| Index of Refraction |
1.653
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| LogP |
2.348
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
272
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(N)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1
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| InChi Key |
UUKWKUSGGZNXGA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H5N3O5/c8-7(11)4-1-5(9(12)13)3-6(2-4)10(14)15/h1-3H,(H2,8,11)
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| Chemical Name |
3,5-dinitrobenzamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 2.2 mg/mL (~10.42 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7364 mL | 23.6821 mL | 47.3642 mL | |
| 5 mM | 0.9473 mL | 4.7364 mL | 9.4728 mL | |
| 10 mM | 0.4736 mL | 2.3682 mL | 4.7364 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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