| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg | |||
| 100mg | |||
| 250mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
β-lactamase enzymes (hydrolyze the β-lactam ring of
|
|---|---|
| ln Vitro |
Michaelis-Menten kinetics of
In bacterial detection assays, Comparison with microtiter plate using UV/vis spectrophotometry gave the same LOD of 10⁶ CFU mL⁻¹ for β-lactamase-expressing E. coli, demonstrating that paper-based In a comparative study of three β-lactamase detection methods, |
| Enzyme Assay |
Reaction optimization on paper: Arrays of 8-mm-diameter paper wells fabricated with filter paper were used. Phosphate buffered saline (PBS) was used as the reaction buffer, and pH was varied to determine optimal pH 7.5. Various concentrations of
Comparison of solution versus dried Nitrocefin test protocol (from Shannon & Phillips): Cell suspensions (10⁸ to 10¹⁰ organisms per mL) were prepared from overnight growth on suitable media. In some cases, cells were subjected to ultrasonic disintegration. |
| Cell Assay |
Detection of β-lactamase-expressing bacteria: Laboratory E. coli expressing β-lactamase were cultured, serially diluted, and mixed with 0.5 mM
Detection in environmental samples: Influent and effluent water samples from a wastewater treatment facility were incubated in growth media for 0-12 hours. Aliquots were taken every 2 hours and tested with Testing bacterial isolates: Ten different bacterial species isolated from environmental samples were tested blindly with In the comparative study (Shannon & Phillips), |
| References |
[2]. Utilizing Paper-Based Devices for Antimicrobial Resistant Bacteria Detection. Angew Chem Int Ed Engl. 2017 May 5. |
| Additional Infomation |
Nitrocefin are chromogenic cephalosporin substrates used to detect the presence of β-lactamases, which are important mediators of bacterial antibiotic resistance. While other detection methods exist, such as PCR, nitrocephalosporin assays can be performed rapidly with minimal reagents and inexpensive equipment. Nitrocefin are β-lactam antibiotics with diagnostic properties. They are sensitive to the hydrolytic activity of all β-lactamases produced by Gram-positive and Gram-negative bacteria. The reagent undergoes a color change when its amide bond is hydrolyzed by β-lactamases, thus making it suitable for studying β-lactamase activity.
Background: Applications: Mechanism: The reaction involves hydrolysis of the carbon-nitrogen bond in the β-lactam ring of |
| Molecular Formula |
C21H16N4O8S2
|
|---|---|
| Molecular Weight |
516.50374
|
| Exact Mass |
516.04
|
| CAS # |
41906-86-9
|
| PubChem CID |
6436140
|
| Appearance |
Yellow to orange solid powder
|
| Density |
1.7±0.1 g/cm3
|
| Boiling Point |
872.0±65.0 °C at 760 mmHg
|
| Melting Point |
>99℃
|
| Flash Point |
481.2±34.3 °C
|
| Vapour Pressure |
0.0±0.3 mmHg at 25°C
|
| Index of Refraction |
1.749
|
| LogP |
1.04
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
35
|
| Complexity |
991
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C1C(=C(N2[C@H](S1)[C@@H](C2=O)NC(=O)CC3=CC=CS3)C(=O)O)/C=C/C4=C(C=C(C=C4)[N+](=O)[O-])[N+](=O)[O-]
|
| InChi Key |
LHNIIDJCEODSHA-OQRUQETBSA-N
|
| InChi Code |
InChI=1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1
|
| Chemical Name |
(6R,7R)-3-[(E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~193.61 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9361 mL | 9.6805 mL | 19.3611 mL | |
| 5 mM | 0.3872 mL | 1.9361 mL | 3.8722 mL | |
| 10 mM | 0.1936 mL | 0.9681 mL | 1.9361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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