| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
In comparison to other biorelevant species including Phe, Gly, Arg, Lys, Tyr, Leu, glucose, Ser, and Val, NIR-Thiol dinitrobenzenesulfonate exhibits a high degree of selectivity towards typical small molecular weight biological thiols (Cys, Hcy, and GSH)[1]. Bright fluorescence is seen when the sensor is incubated with live HeLa or Bel 7702 cells in order to evaluate the sensor's suitability for NIR imaging of thiols in living cells. The sensor seems to be able to image thiols in living cells at near infrared wavelengths and to be permeable to cell membranes. Among the notable characteristics of NIR-Thiol dinitrobenzenesulfonate are its rapid reaction, high sensitivity, great selectivity, good cell membrane permeability, minimal cytotoxicity, and NIR absorption and emission[1].
|
|---|---|
| ln Vivo |
Mice's ip cavities are injected with varying doses of NIR-Thiol dinitrobenzenesulfonate (0, 20, 40, or 160 nanomoles). Mice injected with 20 nanomoles of NIR-Thiol dinitrobenzenesulfonate show strong fluorescence, but control mice receiving no injection of the sensor show essentially little fluorescence at all. Furthermore, animals administered with 40 or 160 nanomoles of NIR-Thiol dinitrobenzenesulfonate exhibit more vivid fluorescence. Mice injected with 20 nanomoles of NIR-Thiol dinitrobenzenesulfonate show an extremely high signal-to-noise contrast ratio (about 700-fold increase). Furthermore, the signal-to-noise contrast ratio increased to about. When 40 or 160 nanomoles of NIR-Thiol dinitrobenzenesulfonate are administered into the mice, respectively, there is a 2900– or 7400–fold enhancement[1].
|
| References |
| Molecular Formula |
C??H??N?O??S?
|
|---|---|
| Molecular Weight |
875.83
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1418 mL | 5.7089 mL | 11.4177 mL | |
| 5 mM | 0.2284 mL | 1.1418 mL | 2.2835 mL | |
| 10 mM | 0.1142 mL | 0.5709 mL | 1.1418 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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