| Size | Price | |
|---|---|---|
| Other Sizes |
Purity: Reagent grade
| Targets |
Nile blue chloride does not have a specific biological target. It is a cationic dye that functions as a fluorescent probe and histological stain. Its fluorescence properties make it useful for various imaging and detection applications. It has been used to detect DNA and lipids and can be utilized to construct ratiometric pH-sensitive probes for real-time tracking of extracellular pH.
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| ln Vitro |
In vitro, Nile blue chloride is used as a fluorescent dye for various applications. It can be used with fluorescein isothiocyanate (FITC) to construct ratiometric pH-sensitive probes. It is also used for nonlinear optical studies and has been used to detect DNA and lipids. Its fluorescence properties, with an absorption maximum (λmax) of 638 nm, make it suitable for fluorescence microscopy and other imaging techniques.
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| ln Vivo |
In vivo applications of Nile blue chloride are not typical, as it is primarily used in vitro as a fluorescent dye and histological stain. It is not administered to living organisms for therapeutic or diagnostic purposes. Its use is confined to laboratory research settings for staining and imaging.
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| Enzyme Assay |
A cell-free assay for Nile blue chloride is not applicable as it is not an enzyme inhibitor. Its properties are characterized by spectroscopic methods. Its fluorescence intensity and pH-dependent color change can be measured in solution. Its ability to bind to DNA and lipids can be assessed in cell-free systems.
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| Cell Assay |
Cellular experiments with Nile blue chloride typically involve staining cells for fluorescence microscopy. Cells are incubated with the dye, and its fluorescence is visualized. It can be used to detect DNA and lipids in cells. The dye's pH sensitivity can also be exploited to measure intracellular pH in living cells.
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| Animal Protocol |
In vivo animal experiments for Nile blue chloride are not applicable, as it is a fluorescent dye used in vitro and ex vivo on cells and tissue sections.
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| ADME/Pharmacokinetics |
Nile blue chloride has a molecular formula of C20H20ClN3O and a molecular weight of 353.85 g/mol. Its absorption maximum is 638 nm. It is a solid powder. Specific pharmacokinetic properties are not applicable as it is not a therapeutic drug. The dye content is typically ≥90%. For storage, it should be kept in a cool, dry place, protected from light.
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| Toxicity/Toxicokinetics |
Toxicity data for Nile blue chloride are not provided in the available sources. As a dye, it should be handled with appropriate safety precautions. It is intended for research use only and is not for human consumption.
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| References | |
| Additional Infomation |
Nile blue A is an organochlorine salt with the para-ion 5-amino-9-(diethylamino)benzo[a]phenoxazine-7-onium. It is a fluorescent dye and a potent photosensitizer for photodynamic therapy. It can be used as a fluorescent dye and a histological dye. It contains Nile blue (1+).
Nile blue chloride (CAS: 2381-85-3) is a highly fluorescent and photostable organic dye. Its synonyms include Nile Blue A and Basic Blue 12. The compound is used as a histological stain, a fluorescent probe, and in nonlinear optical studies. It has been used to detect DNA and lipids. It is not a therapeutic drug and is strictly for research purposes. |
| Molecular Formula |
C20H20CLN3O
|
|---|---|
| Molecular Weight |
353.8453
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| Exact Mass |
353.129
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| CAS # |
2381-85-3
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| Related CAS # |
3625-57-8 (sulfate);53340-16-2 (perchlorate)
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| PubChem CID |
16938
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| Appearance |
Brown to black solid powder
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| Boiling Point |
487.9ºC at 760mmHg
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| Melting Point |
>250°C
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| Flash Point |
248.8ºC
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| LogP |
1.065
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
25
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| Complexity |
625
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SHXOKQKTZJXHHR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H
|
| Chemical Name |
(5-aminobenzo[a]phenoxazin-9-ylidene)-diethylazanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8261 mL | 14.1303 mL | 28.2606 mL | |
| 5 mM | 0.5652 mL | 2.8261 mL | 5.6521 mL | |
| 10 mM | 0.2826 mL | 1.4130 mL | 2.8261 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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