| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
As a labeled compound, N,N-Dimethylglycine-d6 HCl does not have a specific pharmacological target. N,N-Dimethylglycine is involved in one-carbon metabolism and serves as a methyl donor. It has been studied for its potential role in improving immunity and reducing oxidative stress.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, N,N-Dimethylglycine-d6 HCl is used as a tracer to study one-carbon metabolism and methylation reactions. The compound does not exhibit intrinsic pharmacological activity beyond that of N,N-dimethylglycine but serves as a quantitative tool for studying cellular metabolism. |
| ln Vivo |
In vivo, N,N-Dimethylglycine-d6 HCl is used in metabolic studies to trace the absorption, distribution, metabolism, and excretion of N,N-dimethylglycine. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry.
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| Enzyme Assay |
As an analytical standard, N,N-Dimethylglycine-d6 HCl is used in amino acid and methylation studies. Typical protocols involve spiking the labeled compound into samples prior to analysis by GC-MS or LC-MS. The compound serves as an internal standard to correct for matrix effects and instrument variability.
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| Cell Assay |
In vitro cell culture experiments using N,N-Dimethylglycine-d6 HCl involve supplementing growth media with the labeled compound at defined concentrations. Cells are cultured for specified periods, after which the metabolism of the compound is analyzed by mass spectrometry.
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| Animal Protocol |
In vivo animal studies typically involve administering N,N-Dimethylglycine-d6 HCl to rodents via oral gavage or intravenous injection. Blood, urine, and tissue samples are collected at various time points to track the distribution and metabolism of the compound.
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| ADME/Pharmacokinetics |
N,N-Dimethylglycine-d6 HCl exhibits pharmacokinetic properties similar to those of unlabeled N,N-dimethylglycine. As an amino acid derivative, it is absorbed from the gastrointestinal tract and distributed throughout the body. It is metabolized in the liver and excreted in urine. The deuterium label provides a distinct mass shift for analytical detection.
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| Toxicity/Toxicokinetics |
N,N-Dimethylglycine-d6 HCl is considered safe for research use at typical concentrations. The compound is stable under recommended storage conditions. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References | |
| Additional Infomation |
N,N-Dimethylglycine-d6 hydrochloride has a molecular formula of C₄H₄D₆ClNO₂ and a molecular weight of 145.62. The hydrochloride salt form improves stability over the free base. The compound is stored under standard conditions and is primarily used as an internal standard for mass spectrometry-based quantification.
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| Molecular Formula |
C4H9NO2.HCL
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|---|---|
| Molecular Weight |
139.5807
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| Exact Mass |
145.078
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| CAS # |
347840-03-3
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| Related CAS # |
N,N-Dimethylglycine hydrochloride;2491-06-7
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| PubChem CID |
71308968
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| Appearance |
White to off-white solid powder
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| Boiling Point |
190-192ºC(lit.)
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| LogP |
0.434
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
8
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| Complexity |
70.1
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])N(CC(=O)O)C([2H])([2H])[2H].Cl
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| InChi Key |
FKASAVXZZLJTNX-TXHXQZCNSA-N
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| InChi Code |
InChI=1S/C4H9NO2.ClH/c1-5(2)3-4(6)7;/h3H2,1-2H3,(H,6,7);1H/i1D3,2D3;
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| Chemical Name |
2-[bis(trideuteriomethyl)amino]acetic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.1644 mL | 35.8218 mL | 71.6435 mL | |
| 5 mM | 1.4329 mL | 7.1644 mL | 14.3287 mL | |
| 10 mM | 0.7164 mL | 3.5822 mL | 7.1644 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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