| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
N-Methylflindersine targets the production of superoxide, exhibiting potent inhibition against N-formylmethionylleucylphenylalanine-induced superoxide production with IC50 values less than 12 μM. It also shows strong toxicity towards brine shrimp larvae. As an insect antifeedant, it deters feeding in insects.
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|---|---|
| ln Vitro |
In vitro, N-Methylflindersine exhibits potent inhibition against N-formylmethionylleucylphenylalanine-induced superoxide production with IC50 values less than 12 μM. It shows strong toxicity towards brine shrimp larvae with an LD50 of 1.39 μg/ml.
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| ln Vivo |
In vivo, N-Methylflindersine acts as an insect antifeedant. However, specific in vivo efficacy data in mammalian models are not extensively reported.
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| Enzyme Assay |
In vitro assays for N-Methylflindersine involve measuring its inhibition of superoxide production. Cells are stimulated with N-formylmethionylleucylphenylalanine, and superoxide production is measured in the presence and absence of the compound. Toxicity towards brine shrimp larvae is assessed by determining the LD50.
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| Cell Assay |
In vitro cellular assays for N-Methylflindersine are performed using cell lines to study its effects on superoxide production. Cells are treated with the compound and then stimulated to produce superoxide. The inhibition of superoxide production is measured.
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| Animal Protocol |
In vivo animal studies with N-Methylflindersine are not extensively reported. As an insect antifeedant, it may be studied in insect models. However, specific mammalian studies are not detailed.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of N-Methylflindersine are not extensively reported. It has a molecular weight of 241.29 and is almost insoluble in water (0.099 g/L at 25°C). Specific parameters such as oral bioavailability and half-life are not detailed.
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| Toxicity/Toxicokinetics |
The toxicity profile of N-Methylflindersine is indicated by its strong toxicity towards brine shrimp larvae with an LD50 of 1.39 μg/ml. As a research compound, it is for research use only and not for human therapeutic use.
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| References | |
| Additional Infomation |
N-Methyl frindolin is an oxygen heterocyclic compound, an organic heterotricyclic compound, and an organic nitrogen heterocyclic compound. It has been reported to exist in Zanthoxylum bungeanum, Zanthoxylum wutaiense, and other organisms with relevant data.
N-Methylflindersine (CAS 50333-13-6) is an insect antifeedant from Fagara species. It has a molecular formula of C15H15NO2 and a molecular weight of 241.29. The compound exhibits potent inhibition of superoxide production (IC50 <12 μM) and toxicity towards brine shrimp larvae (LD50 1.39 μg/ml). It is available for research purposes only. |
| Molecular Formula |
C15H15NO2
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|---|---|
| Molecular Weight |
241.2851
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| Exact Mass |
241.11
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| CAS # |
50333-13-6
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| PubChem CID |
72819
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
365.8±42.0 °C at 760 mmHg
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| Melting Point |
85℃
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| Flash Point |
175.0±27.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.625
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| Source |
Originated from plants: Rutaceae Fagara chalybea Engl.
Originated from plants: Rutaceae Fagara holtziana Engl. |
| LogP |
2.74
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
18
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| Complexity |
447
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN1C2=C(C=CC=C2)C3=C(C=CC(C)(C)O3)C1=O
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| InChi Key |
RJZFGBNKPOVCHQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H15NO2/c1-15(2)9-8-11-13(18-15)10-6-4-5-7-12(10)16(3)14(11)17/h4-9H,1-3H3
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| Chemical Name |
2,2,6-trimethylpyrano[3,2-c]quinolin-5-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 50 mg/mL (~207.2 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (5.18 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (5.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1444 mL | 20.7220 mL | 41.4439 mL | |
| 5 mM | 0.8289 mL | 4.1444 mL | 8.2888 mL | |
| 10 mM | 0.4144 mL | 2.0722 mL | 4.1444 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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