| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
N-Methyldopamine hydrochloride functions as an agonist of the DA1 receptor. The DA1 receptor is a subtype of dopamine receptor that plays a role in various physiological processes, including cardiovascular function. By binding to and activating this receptor, the compound mimics the effects of dopamine. Additionally, as a precursor of adrenaline in the adrenal medulla, it is involved in the biosynthesis of this critical hormone, which regulates the body's fight-or-flight response.
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| ln Vitro |
N-methyldopamine hydrochloride has faster coating rates and much better surface coating characteristics. Coatings containing N-methyldopamine hydrochloride facilitate solid surface cell adhesion and can be post-functionalized [1].
In vitro, N-Methyldopamine hydrochloride demonstrates agonist activity at the DA1 receptor. Its interaction with the receptor has been characterized in various biochemical assays. The compound's ability to activate the DA1 receptor suggests it can modulate downstream signaling pathways associated with this receptor. Its utility as a surface coating agent also indicates potential for use in material science applications, where its catechol groups can participate in secondary reactions. |
| ln Vivo |
In vivo, N-Methyldopamine hydrochloride is not used as a direct therapeutic agent but is valuable in cardiovascular research, particularly for studying heart failure. Its role as a precursor to adrenaline suggests it could influence adrenergic signaling pathways in living organisms. However, specific in vivo efficacy data and dosing regimens are not detailed in the available sources. The compound is primarily utilized as a research tool to understand DA1 receptor function and catecholamine biosynthesis.
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| Enzyme Assay |
A cell-free assay for N-Methyldopamine hydrochloride would typically involve measuring its binding affinity to the DA1 receptor. In such assays, membrane preparations containing the receptor are incubated with a radiolabeled ligand and varying concentrations of the compound. The concentration required to displace 50% of the specific binding (IC50) can be determined, providing a measure of its receptor affinity. The compound's purity (>98%) and identity are typically confirmed by HPLC and other analytical methods.
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| Cell Assay |
Cellular experiments with N-Methyldopamine hydrochloride would typically involve assessing its effects on DA1 receptor-mediated signaling in cultured cells. Cells expressing the DA1 receptor are treated with the compound, and downstream signaling events, such as changes in cAMP levels or calcium mobilization, are measured. The compound's ability to activate the receptor and elicit a functional response can be quantified, providing a measure of its agonist potency.
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| Animal Protocol |
In vivo animal experiments for N-Methyldopamine hydrochloride are not extensively described in the available sources. As a compound used in cardiovascular research, it could be evaluated in animal models of heart failure to study its effects on cardiac function. However, specific protocols, dosing regimens, and outcome measures are not detailed in the references cited.
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| ADME/Pharmacokinetics |
N-Methyldopamine hydrochloride has a molecular weight of 203.67 g/mol and a molecular formula of C9H14ClNO2. It is typically stored as a powder at -20°C for up to 3 years or at 4°C for up to 2 years. In solution, it can be stored at -20°C for up to 6 months. The compound is soluble in DMSO and other organic solvents. Specific pharmacokinetic parameters, such as half-life and bioavailability, are not applicable as it is a research compound rather than a therapeutic drug.
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| Toxicity/Toxicokinetics |
Specific toxicity data for N-Methyldopamine hydrochloride are not provided in the available sources. As a research compound, it is intended for research use only and is not for human or veterinary use. Standard laboratory safety precautions should be observed when handling this compound, including the use of appropriate personal protective equipment.
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| References | |
| Additional Infomation |
N-Methyldopamine hydrochloride (CAS: 62-32-8) is a chemical modification of dopamine used as a DA1 receptor agonist and a precursor of adrenaline. Its synonyms include N-Methyldopamine HCl and Epinine hydrochloride. The compound is used in cardiovascular research and has potential applications in surface coating technologies. It is not an approved drug and is strictly for research purposes.
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| Molecular Formula |
C9H13NO2.HCL
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| Molecular Weight |
203.66596
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| Exact Mass |
203.071
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| CAS # |
62-32-8
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| PubChem CID |
86470
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
328.6ºC at 760 mmHg
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| Melting Point |
174-176ºC
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| Flash Point |
154.3ºC
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| LogP |
2.052
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
13
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| Complexity |
130
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CNCCC1=CC(=C(C=C1)O)O.Cl
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| InChi Key |
JCDRZCWRRLKLTB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H13NO2.ClH/c1-10-5-4-7-2-3-8(11)9(12)6-7;/h2-3,6,10-12H,4-5H2,1H3;1H
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| Chemical Name |
4-[2-(methylamino)ethyl]benzene-1,2-diol;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~613.74 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9099 mL | 24.5495 mL | 49.0990 mL | |
| 5 mM | 0.9820 mL | 4.9099 mL | 9.8198 mL | |
| 10 mM | 0.4910 mL | 2.4550 mL | 4.9099 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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