| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| ln Vitro |
N-Bis(2-hydroxypropyl)nitrosamine is a chemical that has the potential to cause cancer [1].
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| ln Vivo |
At 100 ppm, rats exposed to N-Bis(2-hydroxypropyl)nitrosamine developed tumors in their thyroid glands, liver, and lungs. Rats at both doses experienced tumors in the lung, liver, thyroid, esophagus, kidney, and bladder in addition to the esophagus, kidney, and bladder at 500 ppm [1]. When rats are stimulated with N-bis(2-hydroxypropyl)nitrosamine (DHPN; 2800 mg/kg), bisphenol A (BPA) increases the susceptibility to thyroid cancer [2].
- In male and female rats, oral administration of N-Bis(2-hydroxypropyl)nitrosamine (DHPN) showed dose-dependent carcinogenic activity. Doses of 100 ppm, 300 ppm, and 600 ppm (in drinking water) induced tumors in multiple organs: liver tumors (hepatocellular carcinomas) occurred in 80–100% of rats at 300 ppm and 600 ppm, renal tumors (renal cell carcinomas) in 40–60% of male rats at 600 ppm, and lung tumors (adenomas) in 20–30% of rats at 300 ppm and 600 ppm. The tumor latency was shortened with increasing doses (e.g., liver tumor latency was ~24 weeks at 600 ppm vs. ~32 weeks at 100 ppm) [1] - In F344 rats, N-Bis(2-hydroxypropyl)nitrosamine (DHPN) combined with excess iodine (in drinking water) induced thyroid carcinoma: rats treated with DHPN (2000 ppm in drinking water for 2 weeks) followed by excess iodine (1000 ppm in drinking water for 40 weeks) had a 45–55% incidence of thyroid follicular carcinoma. Co-administration of low-dose bisphenol A (BPA, 0.1 ppm in drinking water) with DHPN and excess iodine increased the incidence of thyroid follicular carcinoma to 75–85% and promoted tumor progression (larger tumor size, increased invasion) [2] |
| Animal Protocol |
- For dose-dependent carcinogenicity study: Male and female rats were divided into 4 groups (control, 100 ppm DHPN, 300 ppm DHPN, 600 ppm DHPN) with 20 rats per group. N-Bis(2-hydroxypropyl)nitrosamine was dissolved in drinking water and administered ad libitum for 52 weeks. After the treatment period, rats were observed for an additional 24 weeks. Body weight was measured monthly; at the end of the experiment, rats were euthanized, and major organs (liver, kidney, lung, thyroid) were dissected for pathological examination (hematoxylin-eosin staining) to count tumor number and determine tumor type [1]
- For thyroid carcinoma susceptibility study: Male F344 rats (4 weeks old) were divided into 3 groups (n=15 per group): Group 1 (control): normal drinking water; Group 2 (DHPN+I): N-Bis(2-hydroxypropyl)nitrosamine (2000 ppm in drinking water) for 2 weeks, followed by excess iodine (1000 ppm in drinking water) for 40 weeks; Group 3 (DHPN+I+BPA): DHPN (2000 ppm, 2 weeks) + excess iodine (1000 ppm, 40 weeks) + bisphenol A (0.1 ppm in drinking water, 42 weeks total). Rats were weighed weekly; at week 42, all rats were euthanized, and thyroid glands were removed, fixed in formalin, embedded in paraffin, sectioned, and stained with hematoxylin-eosin for pathological diagnosis (tumor type, incidence, size) [2] |
| Toxicity/Toxicokinetics |
N-bis(2-hydroxypropyl)nitrosamine exhibits dose-dependent carcinogenicity in rats: the incidence of liver, kidney, and lung tumors increases significantly with increasing dose (100–600 ppm) [1]
- N-bis(2-hydroxypropyl)nitrosamine (2000 ppm) combined with excess iodine induces thyroid cancer in F344 rats (the incidence of follicular thyroid carcinoma is approximately 50%), and low doses of bisphenol A enhance this carcinogenic effect (increasing the incidence to approximately 80%) [2] |
| References |
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| Additional Infomation |
N,N-bis(2-hydroxypropyl)nitrosamine is a nitrosamine with the structure dipropylamine, in which the hydrogen atom bonded to the nitrogen atom is replaced by a nitroso group. It is a genotoxic carcinogen that primarily damages the lungs, liver, thyroid, and kidneys. It is a carcinogen. It is a nitrosamine, secondary alcohol, and diol.
- N-bis(2-hydroxypropyl)nitrosamine (DHPN) is a well-known chemical carcinogen and is often used in animal models to induce multi-organ tumors (liver, kidney, lung, thyroid) for cancer research [1] - The carcinogenic mechanism of N-bis(2-hydroxypropyl)nitrosamine involves metabolic activation in the liver and target organs, leading to DNA alkylation and mutation, thereby driving tumor development and progression [1] - In thyroid cancer models, N-bis(2-hydroxypropyl)nitrosamine initiates thyroid cell transformation, while excess iodine promotes thyroid cell proliferation (a promoting factor), and low-dose bisphenol A enhances this promoting effect by interfering with thyroid hormone signaling [2] |
| Molecular Formula |
C6H14N2O3
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|---|---|
| Molecular Weight |
162.18696
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| Exact Mass |
162.1
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| CAS # |
53609-64-6
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| PubChem CID |
40828
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| Appearance |
Colorless to light yellow liquid（Density：1.2266 g/cm3）
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
352.4±27.0 °C at 760 mmHg
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| Flash Point |
166.9±23.7 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.497
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| LogP |
-1.2
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
11
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| Complexity |
110
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=NN(CC(O)C)CC(O)C
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| InChi Key |
MNIGYIKCFSPQRJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H14N2O3/c1-5(9)3-8(7-11)4-6(2)10/h5-6,9-10H,3-4H2,1-2H3
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| Chemical Name |
N,N-bis(2-hydroxypropyl)nitrous amide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 125 mg/mL (~770.70 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.1656 mL | 30.8280 mL | 61.6561 mL | |
| 5 mM | 1.2331 mL | 6.1656 mL | 12.3312 mL | |
| 10 mM | 0.6166 mL | 3.0828 mL | 6.1656 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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