| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
| Targets |
N-Acetyl-DL-phenylalanine β-naphthyl ester does not have a specific biological target in a pharmacological sense. It functions as a chromogenic substrate for enzymes such as chymotrypsin and microbial serine proteases. The compound is hydrolyzed by these enzymes at the ester bond, releasing a colored product (β-naphthol) that can be detected spectrophotometrically. It is used to characterize serine proteases and peptidases.
|
|---|---|
| ln Vitro |
N-acetyl-DL-phenylalanine β-naphthyl ester is hydrolyzed in vivo at neutral pH under the catalysis of esterase (APNEase). There is evidence that APNEase may play a role in the turnover and pathological degradation of muscle proteins since it is linked to multiple types of muscular atrophy [1]. The yeast Pichia pastoris Base can be used to directly express N-acetyl-DL-phenylalanine β-naphthyl ester (0.75 mM, DMF; 3 min) as a substrate and o-dianisidine tetrazide (oD) as a dye to assess the protease inhibitory performance [2]. Diazo coupling of β-naphthyl ester of N-acetyl-DL-phenylalanine (0.75 mM, DMF; 3 min) (2.4 g/L) by N-enzyme hydrolysis of β-naphthol-stained agar gel Acetyl-DL-phenylalanine beta-naphthyl ester (APNE) visual results are provided. The region with just the protease shows vivid pink-purple, while the circular region with the inhibitor-protease complex stays colorless [3]. N-acetyl-DL-phenylalanine beta-naphthyl ester (5 mg/40 mL) application: Sorting mouse plasma according to peptidase class. Immunoprecipitation using antisera in radial immunodiffusion allowed for the visualization of the enzyme. Subsequently, each immunoprecipitated ring was incubated in a protease inhibitor solution, cleaned with sufficient physiological saline to eliminate unprecipitated serum and other components, and stained with chromogenic substrates (NAPBNE and Fast Blue B). Straightforward photography in well-lit environments can provide photographs [4].
In vitro, N-Acetyl-DL-phenylalanine β-naphthyl ester is used as a chromogenic substrate in enzymatic assays to measure the activity of chymotrypsin and microbial serine proteases. Upon enzymatic cleavage, the compound releases β-naphthol, which produces a color change that can be monitored. This allows for the quantification of enzyme activity. Specific kinetic parameters are not provided in the available sources. |
| ln Vivo |
In vivo applications of N-Acetyl-DL-phenylalanine β-naphthyl ester are not typical, as it is primarily used in vitro as a chromogenic substrate in enzyme assays. It is not administered to living animals for therapeutic or diagnostic purposes. Its use is confined to laboratory research settings.
|
| Enzyme Assay |
A cell-free assay for N-Acetyl-DL-phenylalanine β-naphthyl ester involves incubating the substrate with a sample containing the enzyme of interest (e.g., chymotrypsin or subtilisin). The enzymatic hydrolysis releases β-naphthol, which can be detected by measuring absorbance at a specific wavelength. The rate of color development is proportional to enzyme activity. Specific assay conditions depend on the enzyme being studied.
|
| Cell Assay |
Cellular experiments with N-Acetyl-DL-phenylalanine β-naphthyl ester are not typical, as it is used as a biochemical reagent in cell-free enzyme assays rather than in cell-based studies.
|
| Animal Protocol |
In vivo animal experiments for N-Acetyl-DL-phenylalanine β-naphthyl ester are not applicable, as it is a chromogenic substrate used in vitro.
|
| ADME/Pharmacokinetics |
N-Acetyl-DL-phenylalanine β-naphthyl ester has a molecular formula of C21H19NO3 and a molecular weight of 333.38. It is a solid compound. Specific pharmacokinetic properties are not applicable as it is not a therapeutic drug. It is used in vitro as a research reagent.
|
| Toxicity/Toxicokinetics |
Toxicity data for N-Acetyl-DL-phenylalanine β-naphthyl ester are not provided in the available sources. As a research reagent, it is intended for research use only and not for human consumption. Standard laboratory safety precautions should be observed when handling this compound.
|
| References |
|
| Additional Infomation |
β-Naphthyl N-acetylphenylalanine ester is an α-amino acid ester obtained by the condensation of N-acetylphenylalanine and 2-naphthol. It is an α-amino acid ester and a derivative of phenylalanine. Its function is related to that of 2-naphthol.
N-Acetyl-DL-phenylalanine β-naphthyl ester (CAS: 20874-31-1) is a chromogenic substrate for chymotrypsin and microbial serine proteases. It is also known as NAPBNE, APNE, and Ac-Phenylalanine β-naphthyl ester. The compound exhibits color changes upon enzymatic cleavage, facilitating the monitoring of enzyme activity. Its molecular formula is C21H19NO3 and its molecular weight is 333.38. It is not a therapeutic drug and is strictly for research purposes. |
| Molecular Formula |
C21H19NO3
|
|---|---|
| Molecular Weight |
333.38046
|
| Exact Mass |
333.136
|
| CAS # |
20874-31-1
|
| PubChem CID |
98192
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.2g/cm3
|
| Boiling Point |
589.8ºC at 760mmHg
|
| Flash Point |
310.5ºC
|
| Vapour Pressure |
6.93E-14mmHg at 25°C
|
| Index of Refraction |
1.618
|
| LogP |
3.883
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
25
|
| Complexity |
458
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(NC(C(OC1=CC2=CC=CC=C2C=C1)=O)CC3=CC=CC=C3)=O
|
| InChi Key |
BBXRRTJNJCPGBU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C21H19NO3/c1-15(23)22-20(13-16-7-3-2-4-8-16)21(24)25-19-12-11-17-9-5-6-10-18(17)14-19/h2-12,14,20H,13H2,1H3,(H,22,23)
|
| Chemical Name |
naphthalen-2-yl 2-acetamido-3-phenylpropanoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9996 mL | 14.9979 mL | 29.9958 mL | |
| 5 mM | 0.5999 mL | 2.9996 mL | 5.9992 mL | |
| 10 mM | 0.3000 mL | 1.4998 mL | 2.9996 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
