| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Targets |
The primary target of N-Acetyl-α-D-glucosamine 1-phosphate is the enzyme UDP-N-acetylglucosamine pyrophosphorylase (also known as GlmU or UAP1). This enzyme catalyzes the conversion of N-acetyl-α-D-glucosamine 1-phosphate and UTP to UDP-N-acetylglucosamine (UDP-GlcNAc) and pyrophosphate. UDP-GlcNAc is a critical sugar nucleotide donor for the biosynthesis of glycosaminoglycans, glycoproteins, and bacterial cell wall components. By serving as a substrate for this enzyme, N-Acetyl-α-D-glucosamine 1-phosphate plays a key role in glycobiology and cellular metabolism.
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| ln Vitro |
In vitro, N-Acetyl-α-D-glucosamine 1-phosphate serves as a substrate for UDP-N-acetylglucosamine pyrophosphorylase. In enzyme activity assays, the compound is incubated with the enzyme, UTP, and appropriate cofactors, and the production of UDP-GlcNAc is measured. The compound's activity as a substrate can be assessed by measuring the rate of UDP-GlcNAc formation using HPLC, mass spectrometry, or coupled enzyme assays. The compound does not exhibit pharmacological activity; its utility is as a biochemical reagent for studying glycosylation pathways.
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| ln Vivo |
N-Acetyl-α-D-glucosamine 1-phosphate does not have in vivo pharmacological activity. It is a biochemical reagent used for research applications, primarily in vitro, to study glycosylation pathways and enzyme mechanisms. The compound is not administered to animals for therapeutic or pharmacological studies. Its use is limited to research applications in glycobiology and biochemistry.
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| Enzyme Assay |
Non-cellular assays for N-Acetyl-α-D-glucosamine 1-phosphate typically involve measuring its activity as a substrate for UDP-N-acetylglucosamine pyrophosphorylase. In a typical assay, the compound is incubated with the enzyme, UTP, and magnesium ions (as a cofactor) in an appropriate buffer. The reaction is allowed to proceed for a defined time, and the production of UDP-GlcNAc is measured by HPLC or by using a coupled enzyme assay with a colorimetric or fluorometric detection system. The kinetic parameters (Km, Vmax) of the enzyme for the substrate can be determined.
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| Cell Assay |
In vitro cellular assays for N-Acetyl-α-D-glucosamine 1-phosphate are not typically conducted for pharmacological evaluation. The compound may be used in cell culture experiments to study glycosylation pathways or to supplement cells with a precursor for glycosaminoglycan biosynthesis. However, the compound is membrane-impermeant and would need to be delivered to cells using specialized methods. Its primary use is as a biochemical reagent for in vitro enzyme assays.
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| Animal Protocol |
N-Acetyl-α-D-glucosamine 1-phosphate is not used in in vivo animal studies as a therapeutic or pharmacological agent. Its application is limited to in vitro research applications in glycobiology and biochemistry. The compound is not administered to animals for therapeutic or pharmacological studies.
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| ADME/Pharmacokinetics |
N-Acetyl-α-D-glucosamine 1-phosphate is not a drug and does not have pharmacokinetic properties. Its physical and chemical properties are more relevant: it is a phosphorylated sugar nucleotide precursor with a molecular weight of 357.14 and a molecular formula of C₈H₁₄NNa₂O₉P. The compound is soluble in water and is typically prepared as an aqueous solution for use in enzyme assays.
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| Toxicity/Toxicokinetics |
Specific toxicological data for N-Acetyl-α-D-glucosamine 1-phosphate are not extensively detailed in standard product information. As a biochemical reagent, it is generally considered to have low toxicity. The compound is a phosphorylated sugar and is expected to be biocompatible. However, standard laboratory safety precautions should be observed when handling this compound. It is not intended for human use.
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| References | |
| Additional Infomation |
N-Acetyl-α-D-glucosamine 1-phosphate is a research-grade biochemical reagent intended for laboratory use only and is not approved for clinical use. Its CAS number is 31281-59-1. The primary applications of N-Acetyl-α-D-glucosamine 1-phosphate include studying the biosynthesis of glycosaminoglycans and glycoproteins, investigating bacterial cell wall synthesis, and serving as a substrate for UDP-N-acetylglucosamine pyrophosphorylase in enzyme assays. The compound is a valuable tool for glycobiology, biochemistry, and drug discovery research.
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| Molecular Formula |
C8H15NNAO9P
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|---|---|
| Molecular Weight |
323.17000
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| Exact Mass |
323.038
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| CAS # |
31281-59-1
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| PubChem CID |
16218869
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
371
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)NC1C(C(C(OC1OP(=O)(O)O)CO)O)O.[Na]
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| InChi Key |
ODFFMCFQIMTMQR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H16NO9P.Na/c1-3(11)9-5-7(13)6(12)4(2-10)17-8(5)18-19(14,15)16;/h4-8,10,12-13H,2H2,1H3,(H,9,11)(H2,14,15,16);
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~724.32 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0943 mL | 15.4717 mL | 30.9435 mL | |
| 5 mM | 0.6189 mL | 3.0943 mL | 6.1887 mL | |
| 10 mM | 0.3094 mL | 1.5472 mL | 3.0943 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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