| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Mucicarmine does not have a specific pharmacological target as it is a histological stain. Its mechanism of action involves binding to acid mucopolysaccharides, specifically mucin, in tissue sections. The stain interacts with the carbohydrate moieties of mucin, resulting in a characteristic deep rose to dark red color. This staining allows for the visualization and identification of mucin-producing cells and mucin-positive tumors in tissue samples. The compound is used as a diagnostic tool in pathology.
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| ln Vitro |
The presence of mucin was successfully detected in frozen tissue sections by mucin staining (mucin stains as a dark rose color). Advice (This is our suggested protocol, which should be adjusted to suit your particular requirements as it simply offers guidance) [1]. 1. Attach cryostat sections (4–5 μm in thickness) to coated glass slides. 2. The sections should be fixed right away using frozen section fixative for 30 seconds, followed by a 5-second water rinse. 3. Submerge the slides for 30 seconds in Harris hematoxylin, then for 5 seconds in each of the following solutions: distilled water, ammonium hydroxide, and distilled water. 4. The mucus red stain should be microwaved for 30 seconds, or until the solution is about to bubble. 5. After giving the slide a two-minute immersion in the warm mucocarmine solution, give it a five-second washing with distilled water. 7. After five seconds of immersion in each of the xylene, 95% alcohol, and 100% alcohol solutions, cover the slides with a slip. NOTE: Nuclear staining (blue) and connective tissue staining (green) may be necessary, depending on your particular needs.
In vitro, Mucicarmine is used for the histological staining of tissue sections. It is specifically used to detect mucin, a glycoprotein present in mucus-producing cells. The stain binds to acid mucopolysaccharides, producing a deep rose to dark red color. This staining is useful in identifying mucin-positive cancers, such as liver carcinoma. The compound is commonly employed in pathology to identify epithelial tumors, particularly in tissue samples from the gastrointestinal tract, lungs, and other mucous-producing organs. Its specificity for mucin makes it a valuable tool for diagnostic pathology. |
| ln Vivo |
Mucicarmine is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a histological stain for in vitro diagnostic use. The compound is used in pathology laboratories to stain tissue sections for the detection of mucin. It is not administered to animals for therapeutic purposes but is used ex vivo for staining and diagnostic applications. Its role is limited to histological analysis.
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| Enzyme Assay |
In vitro staining procedures with Mucicarmine typically involve preparing tissue sections and staining them with the compound. The tissue sections are first deparaffinized and rehydrated. They are then stained with Mucicarmine solution for a specified period, typically 30-60 minutes. The sections are washed and counterstained if necessary. Mucin appears as a deep rose to dark red color. The stained sections are then dehydrated, cleared, and mounted for microscopic examination. The staining results are used to identify mucin-producing cells and mucin-positive tumors.
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| Cell Assay |
In vitro cell-based assays using Mucicarmine are not typical, as the compound is a histological stain used on tissue sections rather than live cells. The stain is applied to fixed tissue sections, not to living cells in culture. Its use is limited to diagnostic pathology and histological analysis. The compound's specificity for mucin makes it a valuable tool for identifying mucin-producing tumors in tissue samples.
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| Animal Protocol |
Mucicarmine is not used for in vivo animal experiments as it is a histological stain. However, tissue samples from animals can be stained with Mucicarmine for histopathological analysis. In such cases, tissues are harvested from animals, fixed, sectioned, and stained with Mucicarmine to detect mucin. The staining protocol is the same as for human tissue samples. The compound is used to identify mucin-positive lesions or tumors in animal models.
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| ADME/Pharmacokinetics |
Mucicarmine has a CAS number of 51395-97-2 and is available as a powder. It appears as an orange to brown to purple powder and has a lambda max of 540.0 to 550.0 nm. For storage, the compound is kept at room temperature. Its solubility and other physicochemical properties are not detailed in the available literature. The compound is intended for research and diagnostic use.
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| Toxicity/Toxicokinetics |
Specific toxicological data for Mucicarmine are not provided in the available sources. As a histological stain, it is intended for in vitro diagnostic use and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of appropriate personal protective equipment. The compound is generally considered safe for use in pathology laboratories when handled properly.
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| References | |
| Additional Infomation |
Mucicarmine is a histological stain used for the visualization of acid mucopolysaccharides in tissue sections. It is commonly used in pathology to detect mucin, a glycoprotein present in mucus-producing cells. Mucicarmine identifies mucin, which appears as a deep rose to dark red color. This staining is useful in determining mucin-positive cancers, such as liver carcinoma. The compound is also known as Mucidye or mucicarmine stain. It appears as an orange to brown to purple powder and has a lambda max of 540.0 to 550.0 nm. Mucicarmine is stored at room temperature and is intended for research and diagnostic use.
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| Molecular Formula |
456.44042G/MOL
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|---|---|
| Molecular Weight |
456.44042g/mol
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| Exact Mass |
1332.447
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| CAS # |
51395-97-2
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| PubChem CID |
170920137
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| Appearance |
Dark purple to black solid powder
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| Hydrogen Bond Donor Count |
18
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| Hydrogen Bond Acceptor Count |
21
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| Rotatable Bond Count |
43
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| Heavy Atom Count |
84
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| Complexity |
2280
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| Defined Atom Stereocenter Count |
13
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| SMILES |
CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CS)NC(=O)[C@H](C[Se])NC(=O)[C@H](CCSC)N
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| InChi Key |
WKDNMZGOOWUCKO-PMIRLTTJSA-N
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| InChi Code |
InChI=1S/C49H86N15O15S4Se/c1-8-23(3)36(46(76)59-30(19-34(51)65)42(72)58-29(48(78)79)12-13-35(66)67)63-41(71)28(15-18-83-7)57-40(70)27(11-10-16-54-49(52)53)56-38(68)25(5)55-43(73)31(20-80)61-47(77)37(24(4)9-2)64-44(74)32(21-81)60-45(75)33(22-84)62-39(69)26(50)14-17-82-6/h23-33,36-37,80-81H,8-22,50H2,1-7H3,(H2,51,65)(H,55,73)(H,56,68)(H,57,70)(H,58,72)(H,59,76)(H,60,75)(H,61,77)(H,62,69)(H,63,71)(H,64,74)(H,66,67)(H,78,79)(H4,52,53,54)/t23-,24-,25-,26-,27-,28-,29-,30-,31-,32-,33-,36-,37-/m0/s1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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