| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
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| ln Vitro |
The presence of mucin was successfully detected in frozen tissue sections by mucin staining (mucin stains as a dark rose color). Advice (This is our suggested protocol, which should be adjusted to suit your particular requirements as it simply offers guidance) [1]. 1. Attach cryostat sections (4–5 μm in thickness) to coated glass slides. 2. The sections should be fixed right away using frozen section fixative for 30 seconds, followed by a 5-second water rinse. 3. Submerge the slides for 30 seconds in Harris hematoxylin, then for 5 seconds in each of the following solutions: distilled water, ammonium hydroxide, and distilled water. 4. The mucus red stain should be microwaved for 30 seconds, or until the solution is about to bubble. 5. After giving the slide a two-minute immersion in the warm mucocarmine solution, give it a five-second washing with distilled water. 7. After five seconds of immersion in each of the xylene, 95% alcohol, and 100% alcohol solutions, cover the slides with a slip. NOTE: Nuclear staining (blue) and connective tissue staining (green) may be necessary, depending on your particular needs.
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|---|---|
| References |
| Molecular Formula |
456.44042G/MOL
|
|---|---|
| Molecular Weight |
456.44042g/mol
|
| Exact Mass |
1332.447
|
| CAS # |
51395-97-2
|
| PubChem CID |
170920137
|
| Appearance |
Dark purple to black solid powder
|
| Hydrogen Bond Donor Count |
18
|
| Hydrogen Bond Acceptor Count |
21
|
| Rotatable Bond Count |
43
|
| Heavy Atom Count |
84
|
| Complexity |
2280
|
| Defined Atom Stereocenter Count |
13
|
| SMILES |
CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CS)NC(=O)[C@H](C[Se])NC(=O)[C@H](CCSC)N
|
| InChi Key |
WKDNMZGOOWUCKO-PMIRLTTJSA-N
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| InChi Code |
InChI=1S/C49H86N15O15S4Se/c1-8-23(3)36(46(76)59-30(19-34(51)65)42(72)58-29(48(78)79)12-13-35(66)67)63-41(71)28(15-18-83-7)57-40(70)27(11-10-16-54-49(52)53)56-38(68)25(5)55-43(73)31(20-80)61-47(77)37(24(4)9-2)64-44(74)32(21-81)60-45(75)33(22-84)62-39(69)26(50)14-17-82-6/h23-33,36-37,80-81H,8-22,50H2,1-7H3,(H2,51,65)(H,55,73)(H,56,68)(H,57,70)(H,58,72)(H,59,76)(H,60,75)(H,61,77)(H,62,69)(H,63,71)(H,64,74)(H,66,67)(H,78,79)(H4,52,53,54)/t23-,24-,25-,26-,27-,28-,29-,30-,31-,32-,33-,36-,37-/m0/s1
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| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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