| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
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| Targets |
MSBN targets thiols (mercaptans, R-SH) in proteins and biological systems. It acts as a highly selective fluorogenic probe that reacts with thiols to produce a significant fluorescence signal. By specifically labeling protein thiols, MSBN enables the detection and study of various reversible protein thiol modifications. Its mechanism is based on a turn-on fluorescence mechanism for selective thiol detection.
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| ln Vitro |
It has been demonstrated that MSBN (5 µM; 0–60 min) reacts with thiols (1 mM) to produce a notable fluorescence signal [1].
In vitro, MSBN (5 µM; 0–60 min) reacts with thiols (1 mM) to produce a notable fluorescence signal. It is a highly selective fluorogenic probe for thiols that can be used to image thiols in live cells selectively and specifically label protein thiols. The compound's turn-on fluorescence mechanism allows for the detection of various reversible protein thiol modifications. |
| ln Vivo |
In vivo data for MSBN are limited. As a fluorogenic probe, its primary application is in in vitro and cell-based studies for imaging and labeling thiols. The compound is not a therapeutic agent and is not used in animal models for drug development.
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| Enzyme Assay |
In vitro assays for MSBN typically involve incubating the compound with samples containing thiols and measuring the resulting fluorescence signal. The compound is dissolved in DMSO and diluted in appropriate buffers. Reactions are carried out at room temperature or 37°C for 0–60 minutes. Fluorescence is measured at appropriate wavelengths. The compound's selectivity for thiols can be confirmed by competition experiments with thiol-blocking agents.
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| Cell Assay |
For cell-based assays, cells are cultured in appropriate media and treated with MSBN at concentrations such as 5 µM. The compound is used to image thiols in live cells selectively. Fluorescence microscopy or flow cytometry can be used to detect the turn-on fluorescence signal. The compound's ability to label protein thiols can be assessed by SDS-PAGE followed by fluorescence scanning.
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| Animal Protocol |
In vivo, MSBN is not typically administered to animals as a therapeutic agent. It is used as a research tool for in vitro and cell-based studies.
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| ADME/Pharmacokinetics |
MSBN (MW 355.41, C₁₇H₁₇NO₄S) is a 1,8-naphthalimide-based fluorogenic probe. Purity: ≥98%. Storage: -20°C, protected from light. Detailed pharmacokinetic parameters are not applicable as it is a probe, not a drug.
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| Toxicity/Toxicokinetics |
MSBN is for research use only and is not intended for human therapeutic applications. Toxicity data are limited. The compound should be handled with standard laboratory safety precautions.
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| References |
[1]. Zhou P, et al. Naphthalimide Scaffold Provides Versatile Platform for Selective Thiol Sensing and Protein Labeling. ACS Chem Biol. 2016 Apr 15;11(4):1098-105.
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| Additional Infomation |
MSBN is a research-grade fluorogenic probe for detecting and imaging thiols. Its primary applications include redox biology, protein modification studies, and chemical biology. The compound is not a drug and has no clinical applications. It is commercially available from various chemical suppliers for research purposes only.
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| Molecular Formula |
C17H17NO4S
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|---|---|
| Molecular Weight |
331.386
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| Exact Mass |
331.09
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| Elemental Analysis |
C, 61.62; H, 5.17; N, 4.23; O, 19.31; S, 9.67
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| CAS # |
135980-66-4
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| PubChem CID |
154730932
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| Appearance |
Solid powder
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| LogP |
2.6
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
594
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCN1C(=O)C2=C3C(=C(C=C2)S(=O)(=O)C)C=CC=C3C1=O
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| InChi Key |
XJWPRGLACNOMLF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H17NO4S/c1-3-4-10-18-16(19)12-7-5-6-11-14(23(2,21)22)9-8-13(15(11)12)17(18)20/h5-9H,3-4,10H2,1-2H3
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| Chemical Name |
2-butyl-6-methylsulfonylbenzo[de]isoquinoline-1,3-dione
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| Synonyms |
MSBN
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~301.76 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0176 mL | 15.0880 mL | 30.1759 mL | |
| 5 mM | 0.6035 mL | 3.0176 mL | 6.0352 mL | |
| 10 mM | 0.3018 mL | 1.5088 mL | 3.0176 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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