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    MM-102
    MM-102

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0400
    CAS #: 1417329-24-8Purity ≥98%

    Description: MM-102 (also known as HMTase Inhibitor IX) is a novel and potent peptidomimetic inhibitor of MLL1 (Mixed lineage leukemia 1) with anticancer activity. It inhibits MLL1 with an IC50 of 0.4 μM in a cell-free assay. MM-102 inhibited the growth of leukemia cells harboring MLL1 fusion proteins. 

    References: J Am Chem Soc. 2013 Jan 16;135(2):669-82.

    Related CAS: 1883545-52-5 (TFA salt)

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    Molecular Weight (MW)669.8
    FormulaC35H49F2N7O4
    CAS No.1417329-24-8
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 100 mg/mL (149.3 mM)      
    Water: N/A
    Ethanol: N/A
    Other info

    Chemical Name: N-[Bis(4-fluorophenyl)methyl]-1-[[(2S)-5-(diaminomethylideneamino)-2-[[2-ethyl-2-(2-methylpropanoylamino)butanoyl]amino]pentanoyl]amino]cyclopentane-1-carboxamide

    InChi Key: RZKSQRIPRKWVBU-MHZLTWQESA-N

    InChi Code: InChI=1S/C35H49F2N7O4/c1-5-34(6-2,43-29(45)22(3)4)31(47)41-27(10-9-21-40-33(38)39)30(46)44-35(19-7-8-20-35)32(48)42-28(23-11-15-25(36)16-12-23)24-13-17-26(37)18-14-24/h11-18,22,27-28H,5-10,19-21H2,1-4H3,(H,41,47)(H,42,48)(H,43,45)(H,44,46)(H4,38,39,40)/t27-/m0/s1

    SMILES Code: O=C(C1(NC([[email protected]@H](NC(C(NC(C(C)C)=O)(CC)CC)=O)CCC/N=C(N)/N)=O)CCCC1)NC(C2=CC=C(F)C=C2)C3=CC=C(F)C=C3

    SynonymsMM102; MM 102; MM-102


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    In Vitro

    In vitro activity: MM-102, as a MLL1 mimetic, shows high binding affinities to WDR5 with IC50 of 2.9 nM and Ki of < 1 nM. In the MLL1-AF9 transduced murine cells, MM-102 specifically reduces expression of two critical MLL1 target genes (HoxA9 and Meis-1), which are required for MLL1 mediated leukemogenesis. In addition, MM-102 effectively and selectively inhibits cell growth and induces apoptosis in leukemia cells harboring MLL1 fusion proteins


    Kinase Assay: The HMT assay is performed in 50 mM HEPES pH 7.8, 100 mM NaCl, 1.0 mM EDTA, and 5% glycerol at 22 °C. Each reaction contains 1.5 μCi of the co-factor, 3H-S-adenosylmethionine. H3 10-residue peptide is used as the substrate at 50 μM. Compounds are added at concentrations ranging from 0.125 to 128 μM and incubated with the pre-assembled WDR5/RbBP5/ASH2L complex at a final concentration of 0.5 μM for each protein for 2–5 min. Reactions are initiated by addition of the MLL1 protein at a final concentration of 0.5 μM and allowed to proceed for 30 min before preparing scintillation counting. To count samples, reactions are spotted on separate squares of P81 filter paper and precipitated by submerging in freshly prepared 50 mM sodium bicarbonate buffer with pH 9.0. After washing and drying, samples are vortexed in Ultima Gold scintillation fluid and counted. As a negative control, assays are performed using 0.5 μM MLL1/WDR5/RbBP5/ASH2L complex assembled with the non-interacting mutant, WDR5D107A.


    Cell Assay: MM-102 significantly inhibited the expression of HoxA9 and Meis-1, two critical MLL1 target genes in MLL1 fusion protein mediated leukemogenesis. MM-102 (6.25, 25, 50 μM) also specifically suppressed cell growth and induced apoptosis in leukemia cells harboring MLL1 fusion proteins. MV4;11, KOPN8, and K562 cells are cultured in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum and 100 U/L penicillin-streptomycin and incubated at 37 °C under 5% CO2. Cells are seeded into 12-well plates for suspension at a density of 5 × 105 per well (1 mL) and treated with either vehicle control (DMSO, 0.2%) or MM-102 for 7 days. The medium is changed every 2 days, and compounds are resupplied. The CellTiter-Glo Luminescent Cell Viability Assay kit is used following the manufacturer’s instruction. First, 100 μL of the assay reagent is added into each well, and the content is mixed for 2 min on an orbital shaker to induce cell lysis. After 10 min incubation at room temperature, the luminescence is read on a microplate reader

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    References

    J Am Chem Soc. 2013 Jan 16;135(2):669-82.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    MM-102
    (a) Selective inhibition of growth of leukemia cell lines with MM-102 treatment for 7 days. (b) Induction of apoptosis by MM-102 in AML cell lines. Cells were treated for 96 h. J Am Chem Soc. 2013 Jan 16;135(2):669-82.



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