| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
TNBC cell death is induced by misetionamide (OTD) (0-2000 μM; 0-72 hours) [2]. Misetionamide (OTD) (0-2000 μM; 12 hours) suppresses the growth of TNBC cells [2]. In TNBC cells, misetionamide (OTD) (0-1000 μM; 18 hours) can cause necrosis and apoptosis [2]. Through a ROS-dependent mechanism, misetionamide (OTD) (250-1000 μM; 18 hours) causes TNBC cell death [2].
|
|---|---|
| Cell Assay |
Cell viability assay [2]
Cell Types: TNBC Cell Tested Concentrations: 0, 100, 200, 500, 1000 and 2000 μM Incubation Duration: 0, 24, 48 and 72 hrs (hours) Experimental Results: TNBC cell death was induced in a dose- and time-dependent manner. Cell proliferation assay [2] Cell Types: TNBC cell Tested Concentrations: 0, 100, 200, 500, 1000 and 2000 μM Incubation Duration: 12 hrs (hours) Experimental Results: Inhibition of TNBC cell proliferation in a dose-dependent manner. Cell viability assay [2] Cell Types: TNBC Cell Tested Concentrations: 250, 500, 750, 1000 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inducing TNBC cell death mainly depends on the production of ROS. Apoptosis analysis [2] Cell Types: TNBC Cell Tested Concentrations: 0, 100, 250, 750, 1000 μM Incubation Duration: 18 hrs (hours) Experimental Results: Induced necrosis and apoptosis of TNBC cells in a dose-dependent manner, inducing apoptosis is a killing Potential mechanisms of cell death in TNBC. |
| References |
[1]. International Nonproprietary Names for Pharmaceutical Substances (INN). WHO Drug Information. 2022. 36(2):337.
[2]. Jinih M, et al. Evaluation of the Cytotoxic Effects of the Novel Antineoplastic Agent 1,4,5-Oxathiazinane-4,4-dioxide on Triple Negative Breast Cancer Cells. Anticancer Res. 2021 May;41(5):2247-2256. |
| CAS # |
856785-75-6
|
|---|---|
| Appearance |
White to off-white solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
