| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Methyl Green zinc chloride targets nucleic acids, specifically DNA and RNA. It binds selectively to DNA, producing a green fluorescence. The compound's mechanism involves intercalation or binding to the major groove of DNA, which allows for the visualization of nucleic acids in gels and tissue sections. It is used as a photochromophore and to activate gelatinous films. At very high pH, methyl green forms an alkaline solution and gets converted to a colorless carbinol base. The dye is used to differentiate bacteria and stain nucleoli and mitochondria in tissues.
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| ln Vitro |
In vitro, Methyl Green zinc chloride is used as a DNA stain for cells and electrophoretic gels. It facilitates direct measurement of cell viability via microscopy and flow cytometry, with fluorescence peaks at 633 nm and 677 nm. In electrophoretic gels, Methyl Green can be used for direct visualization of DNA bands. It serves as a counterstain for various enzyme histochemistry procedures. The dye's stable fluorescence emission and resistance to photobleaching make it ideal for quantitative fluorescence-based assays.
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| ln Vivo |
Methyl Green zinc chloride is not used as a therapeutic agent and has no established in vivo pharmacological activity. It is primarily a research reagent used for staining and fluorescence applications. The compound is a histological dye used to visualize nucleic acids in tissue sections. It can be utilized for chromosome staining through the C-banding technique. As a research tool, Methyl Green is not administered to animals for therapeutic purposes but rather used ex vivo for staining and analytical applications.
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| Enzyme Assay |
In vitro experiments with Methyl Green zinc chloride typically involve preparing staining solutions at concentrations ranging from 0.1% to 1% w/v in appropriate buffers. For DNA staining in gel electrophoresis, the dye is added directly to the gel or used as a post-stain. For cell staining, cells are fixed and incubated with Methyl Green solution for 10-30 minutes, followed by washing steps. Fluorescence is measured using microscopy with excitation at 633 nm and emission at 677 nm. For flow cytometry, stained cells are analyzed using appropriate laser and filter settings. The dye is soluble in water and methanol.
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| Cell Assay |
In vitro cell-based assays using Methyl Green zinc chloride involve staining of cultured cells or tissue sections. Cells are typically fixed with paraformaldehyde or methanol before staining. Methyl Green solution (0.1% w/v) is applied to the fixed cells and incubated for 10-30 minutes at room temperature. After washing with buffer or water, stained cells are visualized by fluorescence microscopy with excitation at 633 nm and emission at 677 nm. For flow cytometry analysis, stained cells are trypsinized, resuspended in buffer, and analyzed using appropriate laser settings. The dye can be used for viability assessment as dead cells may exhibit altered staining patterns.
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| Animal Protocol |
Methyl Green zinc chloride is not typically used for in vivo animal experiments as a therapeutic agent. However, the compound has been used in ex vivo staining of tissues from animal models. For histological analysis, tissues are harvested from animals, fixed, sectioned, and stained with Methyl Green for nuclear visualization. The dye can be used for chromosome staining by C-banding technique in cytogenetic studies. For fluorescence imaging, tissues may be stained with Methyl Green and examined under a fluorescence microscope.
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| ADME/Pharmacokinetics |
Methyl Green zinc chloride does not have established pharmacokinetic properties as it is not used as a therapeutic agent. The compound is a histological dye and fluorescent stain used for research applications. For in vitro use, Methyl Green is soluble in water and methanol, with solutions typically prepared at 0.1% w/v. The dye exhibits fluorescence excitation at 633 nm and emission at 677 nm. For storage, Methyl Green powder should be kept at room temperature, protected from light. Protection from light is imperative to maintain the dye's fluorescence properties.
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| Toxicity/Toxicokinetics |
The toxicity of Methyl Green zinc chloride is primarily associated with its use as a dye rather than as a therapeutic agent. The compound is intended for research use only and is not for human use. As a histological dye, Methyl Green should be handled with appropriate laboratory safety precautions, including the use of gloves and eye protection. The compound may cause irritation to skin, eyes, and respiratory tract upon contact. Inhalation of dust should be avoided. The dye is not classified as a highly toxic substance but should be handled in a well-ventilated area.
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| References | |
| Additional Infomation |
Ethyl green is an imine salt composed of [4-([4-(dimethylamino)phenyl]{4-[ethyl(dimethyl)azono]phenyl}methylene)cyclohexyl-2,5-diene-1-methylene](dimethyl)ammonium, bromide ions, and chloride ions in a 1:1:1 ratio. It is a histological dye used to visualize nucleic acids by Unna-Pappenheim staining coupled with Pyronin Y. It is both a fluorescent dye and a histological dye. It is an organochloride salt, quaternary ammonium salt, imine salt, and organobromine salt. It contains ethyl green (1+).
See also: Methyl green (note moved to). Methyl Green zinc chloride (CAS 7114-03-6) is a basic dicationic dye composed of triphenylmethane. It has the molecular formula C27H35BrClN3·xZnCl2 and a molecular weight of approximately 653.23 g/mol. The compound is a nucleic acid stain used in molecular biology. It binds selectively to DNA, producing a green fluorescence when exposed to ultraviolet light. Methyl Green is suitable for the detection of DNA and RNA and is used in gel electrophoresis and histological applications. It is also used as a pH indicator and an electrophoretic tracking dye. The compound is certified by the Biological Stain Commission and is intended for research use. |
| Molecular Formula |
C27H35BRCL3N3ZN
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|---|---|
| Molecular Weight |
653.2
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| Exact Mass |
649.037
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| CAS # |
7114-03-6
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| PubChem CID |
84671
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| Appearance |
Light green to green solid powder
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| Melting Point |
>300 °C(lit.)
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| LogP |
0.364
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
32
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| Complexity |
645
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC[N+](C)(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-].[Br-]
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| InChi Key |
IDAQSADEMXDTKN-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C27H35N3.BrH.ClH/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;/h9-20H,8H2,1-7H3;2*1H/q+2;;/p-2
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| Chemical Name |
[4-[[4-(dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-ethyl-dimethylazanium;bromide;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~2.5 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5309 mL | 7.6546 mL | 15.3092 mL | |
| 5 mM | 0.3062 mL | 1.5309 mL | 3.0618 mL | |
| 10 mM | 0.1531 mL | 0.7655 mL | 1.5309 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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