The process for making a 0.5% methyl cellulose solution is as follows: weigh 0.5g of dry methyl cellulose and dissolve it in 100ml of ddH2O/0.9% brine (0.9g NaCl in 100ml of ddH2O) to create a transparent solution. The dissolution process can be sped up by gradually adding methyl cellulose to ddH2O/0.9% saline while stirring and heating (between 50 and 65°C). Observations 1. Make sure your methylcellulose solution doesn't have any solid-liquid separation. There are no particles and the solution is transparent and homogeneous. 2. The total dissolution of methyl cellulose may take four hours or longer. It is possible to provide 0.5% MC by injection intraperitoneally or orally [1].

MC (0.5%; catalog number M0430; Sigma-Aldrich) was formulated in sterile water and administered intraperitoneally or PO at a volume of 0.01 ml/g, twice/week for 6 weeks upon acquisition of the fully kindled state. Sterile saline (SAL) was similarly administered (0.01 ml/g). Administration of 0.5% MC or SAL twice/week for 6 weeks commenced 5–7 days after the last stimulation (or sham) needed for all mice to be fully kindled. CKM were randomized to receive either 0.5% MC or SAL by the intraperitoneal (MC: n = 14; SAL: n = 12) or PO (MC: n = 5; SAL: n = 3) route. Sham-kindled mice were similarly randomized to receive either MC or SAL by the intraperitoneal (MC: n = 13; SAL: n = 12) or PO (MC: n = 5; SAL: n = 5) route. CKM were randomized into SAL or MC treatment groups and intraperitoneal or PO administration route groups based on date of acquisition of the fully kindled state such that all groups were balanced to include both mice that achieved the early and those that achieved the late kindling criterion. Sham-kindled mice were randomized within cages. Mice were stimulated or sham-stimulated 24 hours prior to each MC or SAL administration day to ensure the stability of the stage 5 seizure, consistent with our drug-screening protocols in CKM (Barker-Haliski et al., 2016, 2017a; Koneval et al., 2018). The following day, mice were administered either SAL or MC by the assigned route of administration twice weekly for 6 weeks. Health assessments were performed at four time points during the study period by a veterinarian blinded to kindling status and treatment group.[1]
Procedure for preparing 0.5% Methyl cellulose solution
Weigh 0.5g of dry Methyl cellulose and dissolve it in 100ml of ddH2O/0.9% saline solution (0.9g of NaCl dissolved in 100ml of ddH2O) to prepare a clear solution.
Note:
1) Slowly adding Methyl cellulose to ddH2O/0.9% Saline under stirring and heating (50-65 ° C) helps to accelerate dissolution.
2) Make sure there is no solid-liquid separation in the Methyl cellulose solution. The solution is in a uniform and transparent state, with no particles.
3) It may take 4 hours or longer for Methyl cellulose to be completely dissolved.
4) 0.5% Methyl cellulose solution (0.5% MC) can be used for oral administration or intraperitoneal injection.

Absorption, Distribution and Excretion
Cellulose derivatives considered in this report are virtually unabsorbed and little or no degradation of absorbed and little or no degradation of absorbable products occurs in the human digestive tract. In humans, virtually 100 percent of orally ingested methyl cellulose can be recovered in the feces withihn four days, indicating that absorption does not occur.
When swallowed they are not absorbed to any appreciable degree and appear unchanged in feces.
accumulation in liver, spleen, lymph nodes, kidney, and vascular walls.
... Reported that when methylcellulose was given iv to dog and rabbit ... aside from effect upon circulating blood, inability of body to degrade substance led to its retention & accumulation in liver, spleen, lymph nodes, kidney, and vascular walls.
When swallowed they are not absorbed to any appreciable degree and appear unchanged in feces. /Cellulose ethers/

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Metabolism / Metabolites
Reported that when methylcellulose was given iv to dog and rabbit , aside from effect upon circulating blood, inability of body to degrade substance led to its retention & accumulation in liver, spleen, lymph nodes, kidney, and vascular walls.

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Biological Half-Life
4.2 minutes

Interactions
.../METHYLCELLULOSE/ WAS FOUND TO INTERACT WITH SEVERAL COMPD IN ROUGHLY FOLLOWING DECR ORDER: P-HYDROXYBENZOIC ACID, P-AMINOBENZOIC ACID, METHYL P-HYDROXYBENZOATE, PROPYL P-HYDROXYBENZOATE, & BUTYL P-HYDROXYBENZOATE.

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Non-Human Toxicity Values
LD50 Mouse ip 275 g/kg

[1]. Repeated Intraperitoneal Administration of Low-Concentration Methylcellulose Leads to Systemic Histologic Lesions Without Loss of Preclinical Phenotype. J Pharmacol Exp Ther. 2019 Oct;371(1):25-35.

Methyl cellulose polymer consisting of numerous linked glucose molecules used as a stabiliser, thickener and emulsifier for foodstuffs and cosmetics. The Degree of Substitution (DS) of a given form of methyl cellulose is defined as the average number of substituted hydroxyl groups per glucose with a theoretical maximum of 3, however more typical values are 1.3 2.6. Methyl cellulose is a hydrophilic white powder in pure form and dissolves in cold (but not in hot) water, forming a clear viscous solution or gel. It is available under a variety of trade names as a treatment for constipation. Like cellulose, it is not digestible, not toxic, and not allergenic
Methylcellulose is the methyl ether of cellulose with laxative activity. Methylcellulose is not absorbed by the intestines and attracts large amounts of water into the colon, thereby increasing viscosity, producing a softer and bulkier stool and stimulating the constriction of intestinal smooth muscles.
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.

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Drug Indication
Solutions containing methyl cellulose are used as substitute for tears or saliva if the natural production of these fluids is disturbed. It is also used or constipation, diverticulosis, hemorrhoids and irritable bowel syndrome. Used in the manufacture of capsules in nutritional supplements. Its edible and nontoxic properties provide a vegetarian alternative to the use of gelatin.

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Mechanism of Action
Methylcellulose absorbs water in the gastrointestinal lumen thereby increasing the bulk of the stool. This leads to distension and stimulation of peristalsis. The ability of methylcellulose to absorb water may contribute to its efficacy in the management of diarrhea by once again increasing the bulk and consistency of the stool.
TAKEN WITH 1 OR 2 GLASSFUL OF WATER, IT FORMS COLLOIDAL SOLN IN UPPER ALIMENTARY TRACT; THIS SOLN LOSES WATER IN COLON, TO FORM GEL WHICH INCR BULK & SOFTNESS OF STOOL.

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Therapeutic Uses
Cathartics; Pharmaceutic Aids
...IT IS USED AS BULK LAXATIVE IN TREATMENT OF CHRONIC CONSTIPATION.
HYDROPHILIC SUBSTANCES...METHYLCELLULOSE...MAY BE EFFECTIVE IN SYMPTOMATIC TREATMENT OF WATERY DIARRHEA BECAUSE OF THEIR WATER & BILE SALT BINDING CAPACITY.
MEDICATION (VET): LAXATIVE, SUSPENDING AGENT, MITICIDE

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Pharmacodynamics
It increases the bulk in your stool, an effect that helps to cause movement of the intestines. It also works by increasing the amount of water in the stool, making the stool softer and easier to pass.

VARIABLE

variable

658.341

9004-67-5

525128

Off-white to light yellow solid powder

290 to 305 °C /Film/

-1

0

11

12

31

496

0

YLGXILFCIXHCMC-UHFFFAOYSA-N

InChI=1S/C20H38O11/c1-21-9-11-13(23-3)15(24-4)18(27-7)20(30-11)31-14-12(10-22-2)29-19(28-8)17(26-6)16(14)25-5/h11-20H,9-10H2,1-8H3

2,3,4-trimethoxy-6-(methoxymethyl)-5-[3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxyoxane

methylated cellulosecellulose, methyl ether methylcellulose E461

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ~5 mg/mL

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

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Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).

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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)

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Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).

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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders

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Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

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 (Please use freshly prepared in vivo formulations for optimal results.)