| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
Metallo-β-lactamase-IN-6 is a novel VIM-Type metallo-β-lactamase inhibitor with antibacterial activity. It inhibits metallo-β-lactamase with IC50s of 0.56 μM, 29.50 μM and 5.78 μM for VIM-2, VIM-1 and VIM-5. Metallo-β-lactamase-IN-6 displays potent synergistic antibacterial activity with Meropenem against engineered Escherichia coli strains and intractable clinically isolated Pseudomonas aeruginosa producing VIM-2 MBL
| ln Vitro |
Compound 55, metallo-β-lactamase-IN-6 (10 μM; 18–20 hours), has a FIC index value of 0.05 and can enhance Meropenem's antibacterial resistance mediated by VIM-2. Metallo-β-lactamase-IN -6 (1, 10, 100 μM; 18–20 hours) can permeate the outer membrane of E. coli and protect Meropenem from PBP3 by preventing the VIM-2 enzyme's damaging effects on Meropenem[1]. The antibacterial activity of Meropenem against PA W35 is enhanced by metallo-β-lactamase-IN -6 (100 μM) with a FIC index of 0.25[1].
|
|---|---|
| ln Vivo |
The plasma concentration peak of metallo-β-lactamase-IN-6 (100 mg/kg; IP; single) is reached around 9 minutes after injection, with an effective maximum concentration of 142.8 μg/ml. The half-life is 1.24 hours. Mice respond well to metallo-β-lactamase-IN-6 (500, 1000, or 2000 mg/kg; IP; single, observe for 14 days) when dosed at ≤ 2000 mg/kg and does not produce any discernible adverse effects[1]. Metallo-β-lactamase-IN-6 pharmacokinetic parameters in male and female ICR mice [1]. 896 IP (100 mg/kg), T1/2 (h), 1.243 Cmax (μg/mL), 142.8 Tmax (h), 0.151 Vd (mL/kg), 535.804 CL (mL/h/kg), 248.512 AUC0-∞ (μg/mL·h)
|
| Animal Protocol |
Animal/Disease Models: Female ICR mice (180-220 g)[1]
Doses: 100 mg/kg Route of Administration: IP; single (pharmacokinetic/PKs Analysis) Experimental Results: Plasma concentration reached its peak about 9 min after injection with an effective maximum concentration of 142.8 μg/ml, and the T1/2 was 1.24 hrs (hours). Animal/Disease Models: Female ICR mice (n=5)[1] Doses: 500, 1000, or 2000 mg/kg Route of Administration: IP; single, observed for 14 days Experimental Results: Did not result in any significant toxic effects and was well-tolerated by mice at a dose of ≤ 2000 mg/kg. |
| References |
| Molecular Formula |
C10H9N3O2
|
|---|---|
| Molecular Weight |
203.197361707687
|
| Exact Mass |
203.069
|
| CAS # |
1439899-44-1
|
| PubChem CID |
86276926
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
0.4
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
15
|
| Complexity |
229
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
JZQNZUGEXAGUEK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H9N3O2/c14-10(15)9-12-5-6-13(9)7-8-1-3-11-4-2-8/h1-6H,7H2,(H,14,15)
|
| Chemical Name |
1-(pyridin-4-ylmethyl)imidazole-2-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9213 mL | 24.6063 mL | 49.2126 mL | |
| 5 mM | 0.9843 mL | 4.9213 mL | 9.8425 mL | |
| 10 mM | 0.4921 mL | 2.4606 mL | 4.9213 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
