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| 5mg |
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| Targets |
MESG is a substrate for purine nucleoside phosphorylase (PNP), an enzyme involved in purine metabolism. The compound does not have a therapeutic target but is used as a biochemical reagent for detecting inorganic phosphate. In the presence of PNP, MESG is cleaved to release 7-methyl-6-thioguanine and ribose-1-phosphate, with the reaction requiring inorganic phosphate as a substrate.
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| ln Vitro |
In vitro, MESG is a chromogenic substrate used to quantify inorganic phosphate (Pi). When incubated with purine nucleoside phosphorylase (PNP), MESG is converted to 7-methyl-6-thioguanine, which has a different absorbance spectrum. This change in absorbance can be measured spectrophotometrically, allowing the quantification of inorganic phosphate in biological samples. The assay is widely used for measuring phosphate release in enzyme kinetic studies.
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| ln Vivo |
In vivo activity is not applicable for MESG as it is a biochemical reagent used in in vitro assays, not a therapeutic compound. The compound is not administered to animals for pharmacological studies. Its utility lies in providing a sensitive and convenient method for quantifying inorganic phosphate in biochemical and enzymatic assays.
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| Enzyme Assay |
The in vitro phosphate quantification assay using MESG involves incubating the substrate with purine nucleoside phosphorylase (PNP) and the sample containing inorganic phosphate. The assay is typically performed in 96-well plates. MESG is added at a concentration of 0.1-1 mM in assay buffer (e.g., 50 mM HEPES, pH 7.5) containing PNP (0.1-1 U/mL). The sample containing inorganic phosphate is added, and the reaction is incubated at 37degC for 10-30 minutes. The absorbance is measured at 360 nm (for the product 7-methyl-6-thioguanine) or at 330 nm (for the decrease in MESG absorbance). The phosphate concentration is determined from a standard curve.
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| Cell Assay |
In vitro cellular assays using MESG are not typically performed, as the compound is a biochemical reagent for measuring inorganic phosphate in cell-free systems. However, cell lysates or other biological samples can be analyzed for phosphate content using the MESG-PNP assay. Cells are lysed in appropriate buffer, and the lysate is clarified by centrifugation. The clarified lysate is then incubated with MESG and PNP, and the absorbance change is measured to determine phosphate concentration.
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| Animal Protocol |
In vivo animal studies are not relevant for MESG as it is an in vitro research reagent. The compound is not administered to animals for efficacy or pharmacokinetic studies. Its application is restricted to biochemical and enzymatic research for quantifying inorganic phosphate.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for MESG as it is a research reagent used in in vitro assays, not a therapeutic agent. The compound has a molecular weight of 313.33 and a molecular formula of C11H15N5O4S. It should be stored at -20degC. The compound is a modified nucleoside analog used as a tool molecule in biochemical research.
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| Toxicity/Toxicokinetics |
The toxicological profile of MESG is not relevant as it is not a therapeutic compound. As a standard laboratory reagent, it is handled with standard laboratory safety practices. The product is for research use only and is not intended for human therapeutic use. No specific toxicity data have been reported. Standard safety precautions should be followed when handling the compound in a laboratory setting.
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| References | |
| Additional Infomation |
MESG (CAS 55727-10-1), also known as 7-methyl-6-thioguanosine, is a chromophoric substrate used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase (PNP), MESG is converted to 7-methyl-6-thioguanine, resulting in a change in absorbance. The compound is a research tool for measuring phosphate release in enzyme kinetic studies and is available for research purposes only.
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| Molecular Formula |
C11H17N5O4S
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|---|---|
| Molecular Weight |
315.34878
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| Exact Mass |
313.084
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| CAS # |
55727-10-1
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| PubChem CID |
5494430
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| Appearance |
White to yellow solid powder
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| Melting Point |
>175ºC (dec.)
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| LogP |
-1.3
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
21
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| Complexity |
394
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CN1C=[N+](C2=C1C(=NC(=N2)N)[S-])[C@H]3[C@@H]([C@@H]([C@H](O3)CO)O)O
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| InChi Key |
RFHIWBUKNJIBSE-KQYNXXCUSA-N
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| InChi Code |
InChI=1S/C11H15N5O4S/c1-15-3-16(8-5(15)9(21)14-11(12)13-8)10-7(19)6(18)4(2-17)20-10/h3-4,6-7,10,17-19H,2H2,1H3,(H2-,12,13,14,21)/t4-,6-,7-,10-/m1/s1
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| Chemical Name |
2-amino-9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-7-methylpurin-9-ium-6-thiolate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 250 mg/mL (~797.88 mM)
H2O : ≥ 125 mg/mL (~398.94 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1711 mL | 15.8554 mL | 31.7108 mL | |
| 5 mM | 0.6342 mL | 3.1711 mL | 6.3422 mL | |
| 10 mM | 0.3171 mL | 1.5855 mL | 3.1711 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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