| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| 5g | |||
| 10g | |||
| Other Sizes |
| Targets |
MEGA-9 does not have a specific biological target; it is a surfactant. Its mechanism of action is physicochemical, involving the formation of micelles in aqueous solutions to solubilize hydrophobic molecules. It is used for the preparation of lipid bilayers and liposomes for biophysical and biochemical assays. Its high CMC allows for easy removal by dialysis.
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|---|---|
| ln Vitro |
In vitro, MEGA-9 is used as a nonionic detergent to solubilize membrane proteins. Its activity is characterized by its ability to form micelles and solubilize proteins in a native state. It is also used in the preparation of lipid bilayers and liposomes. Its low UV absorbance makes it suitable for protein determination assays.
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| ln Vivo |
In vivo, MEGA-9 is not used as a therapeutic agent. Its primary applications are in vitro for membrane protein research. It is not intended for human consumption or therapeutic use. Any in vivo exposure would be accidental or occupational. Its use is restricted to laboratory and research settings.
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| Enzyme Assay |
In vitro enzyme/receptor binding studies are not applicable to MEGA-9, as it is a detergent rather than a drug. Its activity is assessed by its ability to solubilize membrane proteins without denaturing them. Standard protocols involve incubating membrane preparations with MEGA-9 and assessing protein solubility by centrifugation and SDS-PAGE. Its CMC is determined by surface tension or fluorescence measurements.
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| Cell Assay |
In vitro cellular assays for MEGA-9 are not commonly performed, as it is not a pharmaceutical agent. If cytotoxicity testing is conducted, standard cell viability assays (MTT, neutral red) may be used to assess its effects on cell membranes. However, such studies are not routine, as the compound's primary applications are in protein biochemistry and membrane biology.
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| Animal Protocol |
In vivo animal studies for MEGA-9 are not typically performed, as it is not a drug candidate. If conducted for toxicological assessment, standard acute or subchronic toxicity studies in rodents might be used to evaluate safety for occupational exposure. However, such studies are not well-documented in the literature. The compound is primarily used in laboratory settings.
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| ADME/Pharmacokinetics |
MEGA-9 has a molecular weight of 335.44 and a molecular formula of C₁₆H₃₃NO₆. It is soluble in DMSO (3.36 mg/mL). It has a critical micelle concentration (CMC) of 20 mM under no-salt conditions and CMCs ranging from 2 to 17.1 mM under high and low salt conditions. It is a white solid with a purity of 99.9%.
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| Toxicity/Toxicokinetics |
Toxicological data for MEGA-9 are limited, as it is primarily a laboratory detergent. It may cause skin and eye irritation upon contact. Inhalation of dust should be avoided. At typical laboratory exposure levels, the compound is considered to have low acute toxicity. Standard safety precautions should be followed when handling the compound.
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| Additional Infomation |
MEGA-9 (Nonanoyl-N-Methylglucamide) is a nonionic detergent used for the solubilization of membrane proteins. It is also known as Nonanoyl-N-Methylglucamide. Its high CMC and low UV absorbance make it ideal for protein purification and characterization. It is not approved for human therapeutic use and is intended for research purposes only.
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| Molecular Formula |
C16H33NO6
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|---|---|
| Molecular Weight |
335.441
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| Exact Mass |
335.23
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| CAS # |
85261-19-4
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| PubChem CID |
160090
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
588.6±50.0 °C at 760 mmHg
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| Melting Point |
89-91ºC
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| Flash Point |
309.8±30.1 °C
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| Vapour Pressure |
0.0±3.7 mmHg at 25°C
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| Index of Refraction |
1.523
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| LogP |
0.25
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
13
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| Heavy Atom Count |
23
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| Complexity |
315
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| Defined Atom Stereocenter Count |
4
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| SMILES |
[C@@H](O)([C@@H](O)CN(C)C(=O)CCCCCCCC)[C@H](O)[C@H](O)CO
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| InChi Key |
GCRLIVCNZWDCDE-SJXGUFTOSA-N
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| InChi Code |
InChI=1S/C16H33NO6/c1-3-4-5-6-7-8-9-14(21)17(2)10-12(19)15(22)16(23)13(20)11-18/h12-13,15-16,18-20,22-23H,3-11H2,1-2H3/t12-,13+,15+,16+/m0/s1
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| Chemical Name |
N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
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| Synonyms |
Nonanoyl-N-Methylglucamide; MEGA-9
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9812 mL | 14.9058 mL | 29.8116 mL | |
| 5 mM | 0.5962 mL | 2.9812 mL | 5.9623 mL | |
| 10 mM | 0.2981 mL | 1.4906 mL | 2.9812 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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