| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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||
| 100mg | |||
| Other Sizes |
| Targets |
beta-secretase (BACE1). MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate for measuring protease activity.
|
|---|---|
| ln Vitro |
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate. It is designed for studying protease activity, particularly beta-secretase (BACE1) activity. The peptide contains a fluorescent MCA group and a quenching Dnp group.
|
| ln Vivo |
No specific in vivo activity data is available for MCA-SEVNLDAEFR-K(Dnp)-RR, amide. It is a substrate used in biochemical assays and is not intended as a therapeutic agent.
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| Enzyme Assay |
In vitro enzyme assays using MCA-SEVNLDAEFR-K(Dnp)-RR, amide involve incubating the substrate with the protease of interest (e.g., BACE1). Cleavage of the peptide by the protease separates the MCA fluorophore from the Dnp quencher, resulting in an increase in fluorescence. Fluorescence is measured over time to determine enzyme activity. Kinetic parameters (Km, Vmax) and inhibitor IC50 values can be determined.
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| Cell Assay |
The in vitro cellular activity of MCA-SEVNLDAEFR-K(Dnp)-RR, amide is not typically studied, as it is a substrate used in biochemical assays rather than a cell-active compound. It may be used in cell lysates or conditioned media to measure protease activity.
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| Animal Protocol |
No specific in vivo animal experimental protocols are available for MCA-SEVNLDAEFR-K(Dnp)-RR, amide. It is a biochemical reagent used in in vitro assays and is not administered to animals.
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| ADME/Pharmacokinetics |
MCA-SEVNLDAEFR-K(Dnp)-RR, amide has a molecular formula of C86H125N27O29 and a molecular weight of 2001.08. The sequence is MCA-SEVNLDAEFR-{Lys(Dnp)}-RR-NH2. It is a FRET-based substrate containing a fluorescent MCA group and a quenching Dnp group.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for MCA-SEVNLDAEFR-K(Dnp)-RR, amide. As a research peptide substrate intended for laboratory use, standard safety precautions should be observed.
|
| Additional Infomation |
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate for studying protease activity, particularly beta-secretase (BACE1) activity. It contains a fluorescent MCA group and a quenching Dnp group. It is not approved for clinical use and is intended for research purposes only.
|
| Molecular Formula |
C84H123N27O27
|
|---|---|
| Molecular Weight |
1943.04012
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| Exact Mass |
1941.91
|
| CAS # |
438625-61-7
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| PubChem CID |
145707656
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| Appearance |
Light yellow to yellow solid powder
|
| LogP |
5.966
|
| Hydrogen Bond Donor Count |
30
|
| Hydrogen Bond Acceptor Count |
37
|
| Rotatable Bond Count |
64
|
| Heavy Atom Count |
145
|
| Complexity |
4380
|
| Defined Atom Stereocenter Count |
13
|
| SMILES |
C12OC(C=C(C)C=1C=CC(N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)N)CCCNC(=N)N)CC1C=CC=CC=1)=C2)=O
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.5147 mL | 2.5733 mL | 5.1466 mL | |
| 5 mM | 0.1029 mL | 0.5147 mL | 1.0293 mL | |
| 10 mM | 0.0515 mL | 0.2573 mL | 0.5147 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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