| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
MBS Crosslinker does not have a biological target. It is a chemical reagent used for protein conjugation and crosslinking. The NHS ester reacts with primary amines (e.g., lysine residues) on proteins to form stable amide bonds. The maleimide group reacts with sulfhydryl groups (e.g., cysteine residues) to form stable thioether bonds. This allows for the conjugation of proteins or peptides in a controlled manner.
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|---|---|
| ln Vitro |
In vitro, MBS Crosslinker is used to crosslink proteins by reacting with amine and sulfhydryl groups. It is used for the stabilization of actin filaments in plant tissue samples. It is a non-cleavable, water-insoluble protein crosslinker. Its reactivity is confirmed in protein conjugation studies using model proteins.
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| ln Vivo |
MBS Crosslinker is not a pharmacologically active compound and is not evaluated for in vivo activity. Its applications are limited to in vitro protein crosslinking in biochemical research. No in vivo efficacy studies are conducted because it is not intended to treat any disease.
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| Enzyme Assay |
Cell-free assays for MBS Crosslinker are not typical. Its reactivity can be assessed by measuring the formation of crosslinked products using SDS-PAGE or mass spectrometry. Its purity is confirmed by HPLC (>98.0%). Its molecular weight (314.25) and formula (C15H10N2O6) are confirmed by mass spectrometry.
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| Cell Assay |
Cellular assays are not performed for MBS Crosslinker because it is a reagent rather than a drug candidate. It may be used to crosslink proteins on the cell surface for research purposes, but its effects on cell viability are generally not assessed.
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| Animal Protocol |
MBS Crosslinker is not a pharmacologically active compound and is not evaluated for in vivo therapeutic efficacy. No in vivo animal experiments are conducted because it is not intended for therapeutic use. It is used exclusively in biochemical research for protein crosslinking.
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| ADME/Pharmacokinetics |
MBS Crosslinker has a molecular weight of 314.25 g/mol and a molecular formula of C15H10N2O6. It has a purity of >98.0% (HPLC). It is a solid powder. It is insoluble in water and should be stored under recommended conditions. Its chemical name is N-Succinimidyl 3-Maleimidobenzoate.
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| Toxicity/Toxicokinetics |
The toxicological profile of MBS Crosslinker has not been extensively characterized because it is a research reagent. It may cause irritation upon contact with skin or mucous membranes. Standard safety precautions should be followed. Comprehensive toxicology studies are not necessary.
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| References | |
| Additional Infomation |
MBS Crosslinker is a heterobifunctional crosslinking reagent used for protein conjugation. It is also known as 3-Maleimidobenzoic acid N-hydroxysuccinimide ester. It contains a maleimide group reactive towards sulfhydryls and an NHS ester reactive towards amines. It is a research reagent and is not intended for therapeutic use.
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| Molecular Formula |
C15H10N2O6
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|---|---|
| Molecular Weight |
314.2497
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| Exact Mass |
314.053
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| CAS # |
58626-38-3
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| PubChem CID |
93861
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
524.5±52.0 °C at 760 mmHg
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| Melting Point |
175-177 °C(lit.)
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| Flash Point |
271.0±30.7 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.678
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| LogP |
-1.18
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
592
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C(C1C([H])=C([H])C([H])=C(C=1[H])N1C(C([H])=C([H])C1=O)=O)=O)N1C(C([H])([H])C([H])([H])C1=O)=O
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| InChi Key |
LLXVXPPXELIDGQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H10N2O6/c18-11-4-5-12(19)16(11)10-3-1-2-9(8-10)15(22)23-17-13(20)6-7-14(17)21/h1-5,8H,6-7H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-(2,5-dioxopyrrol-1-yl)benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~318.2 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1822 mL | 15.9109 mL | 31.8218 mL | |
| 5 mM | 0.6364 mL | 3.1822 mL | 6.3644 mL | |
| 10 mM | 0.3182 mL | 1.5911 mL | 3.1822 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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