| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
MBS Crosslinker does not have a biological target. It is a chemical reagent used for protein conjugation and crosslinking. The NHS ester reacts with primary amines (e.g., lysine residues) on proteins to form stable amide bonds. The maleimide group reacts with sulfhydryl groups (e.g., cysteine residues) to form stable thioether bonds. This allows for the conjugation of proteins or peptides in a controlled manner.
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|---|---|
| ln Vitro |
In vitro, MBS Crosslinker is used to crosslink proteins by reacting with amine and sulfhydryl groups. It is used for the stabilization of actin filaments in plant tissue samples. It is a non-cleavable, water-insoluble protein crosslinker. Its reactivity is confirmed in protein conjugation studies using model proteins.
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| ln Vivo |
MBS Crosslinker is not a pharmacologically active compound and is not evaluated for in vivo activity. Its applications are limited to in vitro protein crosslinking in biochemical research. No in vivo efficacy studies are conducted because it is not intended to treat any disease.
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| Enzyme Assay |
Cell-free assays for MBS Crosslinker are not typical. Its reactivity can be assessed by measuring the formation of crosslinked products using SDS-PAGE or mass spectrometry. Its purity is confirmed by HPLC (>98.0%). Its molecular weight (314.25) and formula (C15H10N2O6) are confirmed by mass spectrometry.
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| Cell Assay |
Cellular assays are not performed for MBS Crosslinker because it is a reagent rather than a drug candidate. It may be used to crosslink proteins on the cell surface for research purposes, but its effects on cell viability are generally not assessed.
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| Animal Protocol |
MBS Crosslinker is not a pharmacologically active compound and is not evaluated for in vivo therapeutic efficacy. No in vivo animal experiments are conducted because it is not intended for therapeutic use. It is used exclusively in biochemical research for protein crosslinking.
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| ADME/Pharmacokinetics |
MBS Crosslinker has a molecular weight of 314.25 g/mol and a molecular formula of C15H10N2O6. It has a purity of >98.0% (HPLC). It is a solid powder. It is insoluble in water and should be stored under recommended conditions. Its chemical name is N-Succinimidyl 3-Maleimidobenzoate.
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| Toxicity/Toxicokinetics |
The toxicological profile of MBS Crosslinker has not been extensively characterized because it is a research reagent. It may cause irritation upon contact with skin or mucous membranes. Standard safety precautions should be followed. Comprehensive toxicology studies are not necessary.
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| Additional Infomation |
MBS Crosslinker is a heterobifunctional crosslinking reagent used for protein conjugation. It is also known as 3-Maleimidobenzoic acid N-hydroxysuccinimide ester. It contains a maleimide group reactive towards sulfhydryls and an NHS ester reactive towards amines. It is a research reagent and is not intended for therapeutic use.
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| Molecular Formula |
C15H10N2O6
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|---|---|
| Molecular Weight |
314.2497
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| Exact Mass |
314.053
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| CAS # |
58626-38-3
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| PubChem CID |
93861
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
524.5±52.0 °C at 760 mmHg
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| Melting Point |
175-177 °C(lit.)
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| Flash Point |
271.0±30.7 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.678
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| LogP |
-1.18
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
592
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C(C1C([H])=C([H])C([H])=C(C=1[H])N1C(C([H])=C([H])C1=O)=O)=O)N1C(C([H])([H])C([H])([H])C1=O)=O
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| InChi Key |
LLXVXPPXELIDGQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H10N2O6/c18-11-4-5-12(19)16(11)10-3-1-2-9(8-10)15(22)23-17-13(20)6-7-14(17)21/h1-5,8H,6-7H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-(2,5-dioxopyrrol-1-yl)benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1822 mL | 15.9109 mL | 31.8218 mL | |
| 5 mM | 0.6364 mL | 3.1822 mL | 6.3644 mL | |
| 10 mM | 0.3182 mL | 1.5911 mL | 3.1822 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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