| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
m-PEG6-alcohol does not target a biological receptor. Its function is as a chemical intermediate or linker. The terminal hydroxyl group can be derivatized to introduce other reactive functional groups (e.g., tosylate, mesylate, amine, carboxylic acid). In bioconjugation, the PEG6 spacer provides hydrophilicity and flexibility to the final conjugate.
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|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
As a chemical intermediate, m-PEG6-alcohol has no intrinsic biological activity. Its utility is defined by its terminal hydroxyl group, which can participate in various chemical reactions including etherification, esterification, and oxidation. The PEG6 spacer provides excellent water solubility and acts as a humectant in formulations. |
| ln Vivo |
No direct in vivo activity is attributed to m-PEG6-alcohol. It functions as a building block for synthesizing more complex PEG derivatives and bioconjugates. The hydroxyl group can be converted to reactive handles for subsequent conjugation reactions. The PEG6 spacer improves solubility and reduces immunogenicity of final conjugates.
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| Enzyme Assay |
For non-cell-based assays, m-PEG6-alcohol is characterized by GC (≥97% purity) and NMR. The hydroxyl group can be quantified by titration or derivatization followed by GC or HPLC. Physical properties including refractive index (1.45) and density are measured. The compound's solubility in various solvents can be assessed.
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| Cell Assay |
Cell-based assays are not performed on m-PEG6-alcohol. It is a synthetic intermediate used to prepare functionalized PEG derivatives or bioconjugates, which are then evaluated in cell culture. Standard protocols for testing the resulting conjugates involve treating cells at 0.001-10 µM for 24-72 hours, with assessment of relevant biological endpoints.
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| Animal Protocol |
In vivo studies with m-PEG6-alcohol are not typically conducted on the compound itself. It is used to synthesize PEGylated drugs or bioconjugates, which are then evaluated in animal models. Typical protocols involve administering the PEGylated product to rodents via various routes at therapeutic doses. The PEG6 spacer contributes to prolonged circulation half-life.
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| ADME/Pharmacokinetics |
As a chemical intermediate, m-PEG6-alcohol does not have established PK properties. The compound is hydrophilic with a density of 1.08 g/cm³. When incorporated into a PEGylated drug, the PEG6 chain improves aqueous solubility, reduces renal clearance, and extends circulation half-life. Storage at 4°C under nitrogen is recommended.
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| Toxicity/Toxicokinetics |
Toxicology data specific to m-PEG6-alcohol are limited. The compound is handled with standard laboratory safety practices. PEG derivatives are generally considered biocompatible and non-toxic. No significant acute toxicity is expected. Store at -20°C for long-term stability.
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| References | |
| Additional Infomation |
Hexaethylene glycol monomethyl ether is a hydroxyl polyether. It is functionally related to hexaethylene glycol.
See also: PEG-6 methyl ether (note moved to). m-PEG6-alcohol is a PEG linker containing a hydroxyl group. Synonyms include hexaethylene glycol monomethyl ether. The compound is used as a solvent and humectant in cosmetic formulations. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. No clinical trials exist. |
| Molecular Formula |
C13H28O7
|
|---|---|
| Molecular Weight |
296.3572
|
| Exact Mass |
296.183
|
| CAS # |
23601-40-3
|
| Related CAS # |
23601-40-3;
|
| PubChem CID |
90207
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
375.6±37.0 °C at 760 mmHg
|
| Flash Point |
180.9±26.5 °C
|
| Vapour Pressure |
0.0±1.9 mmHg at 25°C
|
| Index of Refraction |
1.446
|
| LogP |
-2.59
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
17
|
| Heavy Atom Count |
20
|
| Complexity |
169
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])O[H])C([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])[H]
|
| InChi Key |
FHHGCKHKTAJLOM-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C13H28O7/c1-15-4-5-17-8-9-19-12-13-20-11-10-18-7-6-16-3-2-14/h14H,2-13H2,1H3
|
| Chemical Name |
2-[2-[2-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethanol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3743 mL | 16.8714 mL | 33.7427 mL | |
| 5 mM | 0.6749 mL | 3.3743 mL | 6.7485 mL | |
| 10 mM | 0.3374 mL | 1.6871 mL | 3.3743 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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