| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Lysyl-tyrosyl-lysine does not have a single well-defined molecular target like a receptor or enzyme. Its biological activities are attributed to its properties as a peptide derived from extracellular matrix proteins. It is known to possess anti-inflammatory, antioxidant, and antimicrobial properties.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro, Lysyl-tyrosyl-lysine exhibits several biological activities. It has been shown to possess anti-inflammatory, antioxidant, and antimicrobial properties. These activities have been demonstrated in various cell-based assays. As a tripeptide found in collagen and elastin, it may also play a role in cell signaling and tissue repair processes. |
| ln Vivo |
In vivo, Lysyl-tyrosyl-lysine is a naturally occurring peptide found in collagen and elastin. Its biological activities, such as anti-inflammatory and antioxidant effects, suggest potential roles in tissue maintenance and repair. However, specific in vivo data for the isolated peptide is not detailed in the available literature.
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| Enzyme Assay |
Cell-free assays for Lysyl-tyrosyl-lysine are not the primary method for characterizing its activity. As a peptide, its properties can be studied using standard biochemical techniques. Its antioxidant activity, for example, could be assessed in cell-free systems using assays like DPPH radical scavenging. Its anti-inflammatory properties could be studied via enzyme inhibition assays.
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| Cell Assay |
In vitro cell-based assays for Lysyl-tyrosyl-lysine are performed to study its biological activities. For example, its anti-inflammatory activity can be assessed in macrophage cell lines by measuring the production of inflammatory cytokines (e.g., TNF-α, IL-6) upon treatment. Its antioxidant activity can be evaluated by measuring the reduction of reactive oxygen species (ROS) in cells. Antimicrobial activity can be tested against bacterial cultures.
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| Animal Protocol |
In vivo animal experiments for Lysyl-tyrosyl-lysine are not extensively documented in the available literature. As a naturally occurring peptide found in collagen and elastin, it may be studied in models of wound healing or inflammation. However, specific protocols for the isolated tripeptide are not detailed.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) data for Lysyl-tyrosyl-lysine is not available in the public literature. As a peptide, its oral bioavailability would likely be low due to degradation in the gastrointestinal tract. For research use, it is typically stored as a powder at -20°C.
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| Toxicity/Toxicokinetics |
Toxicological data for Lysyl-tyrosyl-lysine is not available in the public literature. As a naturally occurring peptide found in collagen and elastin, it is generally considered to be biocompatible. However, specific toxicity studies have not been detailed. Its use is for research purposes only.
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| References | |
| Additional Infomation |
Lys-Tyr-Lys is an oligopeptide.
Lysyl-tyrosyl-lysine is a research-grade bioactive tripeptide. It is composed of lysine, tyrosine, and lysine residues and is found in collagen and elastin. It has been reported to possess anti-inflammatory, antioxidant, and antimicrobial properties. It has not been approved for clinical use. All information is for research reference only. |
| Molecular Formula |
C21H35N5O5
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|---|---|
| Molecular Weight |
437.53
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| Exact Mass |
497.285
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| CAS # |
35193-18-1
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| PubChem CID |
147602
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| Appearance |
White to off-white solid powder
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| Density |
1.236g/cm3
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| Boiling Point |
821.3ºC at 760mmHg
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| Flash Point |
450.5ºC
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| LogP |
2.547
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
31
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| Complexity |
554
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCCCN)N)O
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| InChi Key |
WINFHLHJTRGLCV-BZSNNMDCSA-N
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| InChi Code |
InChI=1S/C21H35N5O5/c22-11-3-1-5-16(24)19(28)26-18(13-14-7-9-15(27)10-8-14)20(29)25-17(21(30)31)6-2-4-12-23/h7-10,16-18,27H,1-6,11-13,22-24H2,(H,25,29)(H,26,28)(H,30,31)/t16-,17-,18-/m0/s1
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| Chemical Name |
(2S)-6-amino-2-[[(2S)-2-[[(2S)-2,6-diaminohexanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~228.56 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2856 mL | 11.4278 mL | 22.8556 mL | |
| 5 mM | 0.4571 mL | 2.2856 mL | 4.5711 mL | |
| 10 mM | 0.2286 mL | 1.1428 mL | 2.2856 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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