| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Lumiflavine targets the riboflavin uptake mechanism in epithelial cells. It acts as a riboflavin uptake inhibitor, blocking the transport of riboflavin (vitamin B2) into cells. As a riboflavin analog, it may also serve as a precursor and coenzyme in various enzymatic reactions involving flavoproteins.
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|---|---|
| ln Vitro |
In vitro, lumiflavine is used to study riboflavin uptake in intestinal epithelial (Caco-2) and other epithelial cells. It inhibits the uptake of riboflavin, allowing researchers to investigate the mechanisms of riboflavin transport and metabolism. Detailed in vitro activity data is limited.
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| ln Vivo |
In vivo activity data for lumiflavine is limited. As a riboflavin uptake inhibitor, it is primarily used as a research tool in cell-based assays rather than for in vivo applications.
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| Enzyme Assay |
Non-cellular assays for lumiflavine are not applicable as it is a riboflavin uptake inhibitor rather than an enzyme inhibitor. The compound's identity and purity are confirmed by standard analytical methods such as NMR and mass spectrometry.
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| Cell Assay |
In vitro cellular experiments involve culturing intestinal epithelial cells (e.g., Caco-2) or other epithelial cells in the presence of lumiflavine. Riboflavin uptake is measured using radiolabeled riboflavin or fluorescently labeled riboflavin analogs. The inhibition of riboflavin uptake by lumiflavine is quantified and compared to control conditions.
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| Animal Protocol |
In vivo animal studies for lumiflavine have not been extensively reported. The compound is primarily used as a research tool in cell-based assays to study riboflavin transport mechanisms.
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| ADME/Pharmacokinetics |
Lumiflavine is not a drug and does not have pharmacokinetic properties. It has a molecular weight of 256.26 g/mol and a molecular formula of C₁₃H₁₂N₄O₂. The compound is an orange crystalline solid with a melting point of 320-330°C. It is freely soluble in chloroform and very sparingly soluble in water and most organic solvents.
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| Toxicity/Toxicokinetics |
Lumiflavine has low toxicity. The compound is for research purposes only and should be handled with appropriate safety precautions.
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| References | |
| Additional Infomation |
Lumiflavin is a compound that exhibits yellow-green fluorescence and is formed by the photolysis of riboflavin in an alkaline solution. It has been reported that long-stemmed yucca (Securidaca longipedunculata) contains Lumiflavin, and relevant data are available for reference.
Lumiflavine is a riboflavin analog and a riboflavin uptake inhibitor used to study riboflavin uptake in intestinal epithelial (Caco-2) and other epithelial cells. It has applications in biochemistry and enzymology. This product is for research purposes only and is not for human therapeutic use. |
| Molecular Formula |
C13H12N4O2
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|---|---|
| Molecular Weight |
256.27
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| Exact Mass |
256.096
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| CAS # |
1088-56-8
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| PubChem CID |
66184
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Index of Refraction |
1.729
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| LogP |
0.23
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
19
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| Complexity |
511
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KPDQZGKJTJRBGU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H12N4O2/c1-6-4-8-9(5-7(6)2)17(3)11-10(14-8)12(18)16-13(19)15-11/h4-5H,1-3H3,(H,16,18,19)
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| Chemical Name |
7,8,10-trimethylbenzo[g]pteridine-2,4-dione
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| Synonyms |
Lumiflavine Lumilactoflavin Lumiflavin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
0.1 M NaOH : ~25 mg/mL (~97.56 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9021 mL | 19.5107 mL | 39.0213 mL | |
| 5 mM | 0.7804 mL | 3.9021 mL | 7.8043 mL | |
| 10 mM | 0.3902 mL | 1.9511 mL | 3.9021 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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