| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
The specific molecular targets of Licoricesaponin A3 have not been well-characterized. As a triterpene saponin, it may interact with cell membranes, affecting membrane fluidity and permeability, and may modulate various cellular signaling pathways. Saponins are known for their ability to form complexes with cholesterol and other membrane components, which can affect cell membrane integrity and function. Licoricesaponin A3 may also have immunomodulatory, anti-inflammatory, or antioxidant activities, as other licorice saponins, such as glycyrrhizin, have been shown to possess these properties. However, specific studies on the molecular targets of Licoricesaponin A3 are lacking.
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|---|---|
| ln Vitro |
In vitro, Licoricesaponin A3 has been studied for its cytotoxic activity against human cancer cell lines. In one study, the compound showed cytotoxic activity against the human cancer cell lines MGC-803 (gastric cancer), SW620 (colon cancer), and SMMC-7721 (liver cancer), with IC50 values greater than 100 μmol/L. This indicates that the compound has only weak to moderate cytotoxic activity against these cell lines. In addition to its cytotoxic activity, Licoricesaponin A3 may have other in vitro activities, such as antioxidant or anti-inflammatory effects, but these have not been extensively studied. The compound's in vitro activity is likely influenced by its glycosidic moiety, which may affect its solubility, cellular uptake, and interaction with cellular targets.
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| ln Vivo |
In vivo, the biological activities of Licoricesaponin A3 have not been extensively studied. As a saponin, it may have low oral bioavailability due to its glycosidic structure and high molecular weight. The compound may be metabolized in the gut by intestinal bacteria to release the aglycone glycyrrhetic acid, which is the major bioactive metabolite of licorice saponins. However, specific in vivo studies on Licoricesaponin A3 are lacking in the available literature.
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| Enzyme Assay |
The non-cellular assay for Licoricesaponin A3 would likely involve the measurement of its cytotoxic activity using cell-free systems, such as enzyme inhibition assays or antioxidant assays. However, specific protocols for Licoricesaponin A3 have not been reported.
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| Cell Assay |
The cellular assay for Licoricesaponin A3 involves treating cultured cancer cell lines with the compound and measuring cell viability using standard assays such as MTT or CCK-8. Cells are seeded in multi-well plates and treated with Licoricesaponin A3 at various concentrations for 24-72 hours. Cell viability is measured, and the IC50 is determined from concentration-response curves. In addition to cytotoxicity, the effects of Licoricesaponin A3 on cell cycle progression, apoptosis, and cell migration can be studied. However, specific data on these endpoints are limited.
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| Animal Protocol |
In vivo animal studies for Licoricesaponin A3 have not been reported in the available literature. As a research compound, it may be studied in models of cancer or inflammation, but specific protocols and findings are lacking.
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| ADME/Pharmacokinetics |
Licoricesaponin A3 has a molecular weight of 985.07 g/mol and a molecular formula of C₄₈H₇₂O₂₁. The compound is a triterpene saponin with high molecular weight and polar glycosidic groups, which contribute to its hydrophilic character. It is soluble in water and polar organic solvents. The compound should be stored at 2-8°C, protected from light and moisture, and is stable for up to 24 months when stored properly.
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| Toxicity/Toxicokinetics |
The toxicological profile of Licoricesaponin A3 has not been extensively characterized. As a saponin, it may have hemolytic activity and can cause gastrointestinal disturbances at high doses. The compound should be handled with appropriate laboratory safety precautions.
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| References | |
| Additional Infomation |
It has been reported that Licoricesaponin A3 is present in licorice (Glycyrrhiza uralensis) and inflatable licorice (Glycyrrhiza inflata), and relevant data are available for reference. See also: Licorice (Glycyrrhiza uralensis) root (part).
Licoricesaponin A3 is a triterpene saponin isolated from licorice root that has been studied for its cytotoxic activity against human cancer cell lines. Although its cytotoxic activity is relatively weak (IC50 > 100 μmol/L), it is of interest as a naturally occurring compound with potential biological activities. Licoricesaponin A3 is one of many bioactive saponins found in licorice, a plant that has been used in traditional medicine for centuries. Further research is needed to fully characterize the pharmacological properties and potential therapeutic applications of Licoricesaponin A3. |
| Molecular Formula |
C48H72O21
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|---|---|
| Molecular Weight |
985.08
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| Exact Mass |
984.457
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| CAS # |
118325-22-7
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| PubChem CID |
14187172
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| Appearance |
White to light yellow solid powder
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| LogP |
1.9
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| Hydrogen Bond Donor Count |
11
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| Hydrogen Bond Acceptor Count |
21
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
69
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| Complexity |
2050
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| Defined Atom Stereocenter Count |
24
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| SMILES |
C[C@]12CC[C@](C[C@H]1C3=CC(=O)[C@@H]4[C@]5(CC[C@@H](C([C@@H]5CC[C@]4([C@@]3(CC2)C)C)(C)C)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)C(=O)O)O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)C(=O)O)O)O)O)C)(C)C(=O)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O
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| InChi Key |
HJFOOTRGDAPZMV-SMVKYPPISA-N
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| InChi Code |
InChI=1S/C48H72O21/c1-43(2)23-8-11-48(7)36(21(50)16-19-20-17-45(4,13-12-44(20,3)14-15-47(19,48)6)42(63)69-39-31(57)26(52)25(51)22(18-49)64-39)46(23,5)10-9-24(43)65-41-35(30(56)29(55)34(67-41)38(61)62)68-40-32(58)27(53)28(54)33(66-40)37(59)60/h16,20,22-36,39-41,49,51-58H,8-15,17-18H2,1-7H3,(H,59,60)(H,61,62)/t20-,22+,23-,24-,25+,26-,27-,28-,29-,30-,31+,32+,33-,34-,35+,36+,39-,40-,41+,44+,45-,46-,47+,48+/m0/s1
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| Chemical Name |
(2S,3S,4S,5R,6R)-6-[(2R,3R,4S,5S,6S)-2-[[(3S,4aR,6aR,6bS,8aS,11S,12aR,14aR,14bS)-4,4,6a,6b,8a,11,14b-heptamethyl-14-oxo-11-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxycarbonyl-2,3,4a,5,6,7,8,9,10,12,12a,14a-dodecahydro-1H-picen-3-yl]oxy]-6-carboxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid
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| Synonyms |
Licorice-saponin A3; Licoricesaponin A3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0151 mL | 5.0757 mL | 10.1515 mL | |
| 5 mM | 0.2030 mL | 1.0151 mL | 2.0303 mL | |
| 10 mM | 0.1015 mL | 0.5076 mL | 1.0151 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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