| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Leukotriene B4 stimulates neutrophil, monocyte, and eosinophil recruitment and activation. It can intensify and extend tissue inflammation as evidenced by the numerous pro-inflammatory cytokines and mediators that it boosts the production of [2]. Bone marrow cells produce leukotriene B4, a pro-inflammatory mediator, from arachidonic acid [2].
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| References |
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| Additional Infomation |
Leukotriene B4 is a leukotriene composed of (6Z,8E,10E,14Z)-eicosatetraenoic acid and its (5S)- and (12R)-hydroxy substituents. It is an inflammatory lipid mediator produced from arachidonic acid via the 5-lipoxygenase pathway. It is present in humans, mice, and plants and has vasoconstrictive effects. It is a dihydroxy monocarboxylic acid, leukotriene, long-chain fatty acid, and hydroxy polyunsaturated fatty acid. Its function is associated with eicosatetraenoic acid (6,8,10,14-tetraenoic acid). It is the conjugate acid of leukotriene B4(1-). Leukotriene B4 has been used in clinical trials investigating the treatment of HIV infection. Leukotriene B4 is present in or produced by Escherichia coli (K12 strain, MG1655 strain). There are reports of the presence of leukotriene B4 in humans, and relevant data are available. Leukotriene B4 is a leukotriene synthesized from leukotriene A4 by leukotriene A4 hydrolase in monocytes, lymphocytes, neutrophils, reticulocytes, platelets, and fibroblasts. Leukotriene B4 regulates inflammation by promoting neutrophil activation and inducing leukocyte adhesion, activation, and transendothelial migration. Leukotriene B4 is a metabolite found or produced in Saccharomyces cerevisiae. It is a major metabolite of neutrophils. It stimulates the function of polymorphonuclear cells (degranulation, formation of oxygen free radicals, release and metabolism of arachidonic acid). (Excerpt from Dictionary of Prostaglandins and Related Compounds, 1990)
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| Molecular Formula |
C20H32O4
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|---|---|
| Molecular Weight |
336.46568
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| Exact Mass |
336.23
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| CAS # |
71160-24-2
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| Related CAS # |
Leukotriene B4-d4;124629-74-9
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| PubChem CID |
5280492
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
536.4±50.0 °C at 760 mmHg
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| Melting Point |
-144.0 °C (lit.)
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| Flash Point |
292.3±26.6 °C
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| Vapour Pressure |
0.0±3.2 mmHg at 25°C
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| Index of Refraction |
1.527
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| LogP |
4.06
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
14
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| Heavy Atom Count |
24
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| Complexity |
421
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CCCCC/C=C\C[C@H](/C=C/C=C/C=C\[C@H](CCCC(=O)O)O)O
|
| InChi Key |
VNYSSYRCGWBHLG-AMOLWHMGSA-N
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| InChi Code |
InChI=1S/C20H32O4/c1-2-3-4-5-6-9-13-18(21)14-10-7-8-11-15-19(22)16-12-17-20(23)24/h6-11,14-15,18-19,21-22H,2-5,12-13,16-17H2,1H3,(H,23,24)/b8-7+,9-6-,14-10+,15-11-/t18-,19-/m1/s1
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| Chemical Name |
(5S,6Z,8E,10E,12R,14Z)-5,12-dihydroxyicosa-6,8,10,14-tetraenoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9720 mL | 14.8602 mL | 29.7203 mL | |
| 5 mM | 0.5944 mL | 2.9720 mL | 5.9441 mL | |
| 10 mM | 0.2972 mL | 1.4860 mL | 2.9720 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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