| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
Leptin (93-105) is a fragment of the full-length leptin hormone, which primarily acts through the leptin receptor (LEPR or Ob-R) in the hypothalamus. The leptin receptor is a class I cytokine receptor that signals through the JAK-STAT pathway to regulate energy homeostasis, food intake, and body weight.
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| ln Vitro |
In vitro, leptin and its fragments are studied for their effects on cell signaling pathways, including STAT3 phosphorylation, in leptin-responsive cell lines. The full-length hormone inhibits food intake and stimulates energy expenditure in cellular models. Fragment-specific activities may vary compared to the full-length protein.
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| ln Vivo |
In vivo, full-length leptin decreases food intake and body weight in animal models, particularly in leptin-deficient ob/ob mice. Leptin (93-105) as a fragment may be used to study specific regions of the leptin protein responsible for receptor binding or biological activity, though its independent in vivo activity is less characterized.
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| Enzyme Assay |
Receptor binding studies for leptin fragments typically use radioligand binding assays with membrane preparations from cells expressing the leptin receptor. Radiolabeled leptin is incubated with varying concentrations of the test peptide, and binding affinity is determined by displacement curves.
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| Cell Assay |
Cell-based assays for leptin fragments involve culturing leptin-responsive cell lines such as neuronal cells or hypothalamic cell models. Cells are treated with the peptide, and downstream signaling markers such as STAT3 phosphorylation are measured by Western blot or ELISA to assess receptor activation.
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| Animal Protocol |
In vivo studies with leptin fragments are typically conducted in rodent models, often using leptin-deficient ob/ob mice. The peptide is administered via intraperitoneal or intravenous injection, and food intake, body weight, and metabolic parameters are monitored over time.
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| ADME/Pharmacokinetics |
As a peptide fragment, Leptin (93-105) is expected to have a short half-life due to proteolytic degradation. The full-length leptin hormone has a half-life of approximately 25-40 minutes in circulation. The fragment should be stored as a lyophilized powder at -20degC or below.
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| Toxicity/Toxicokinetics |
Toxicity data for Leptin (93-105) are limited. The full-length leptin hormone is generally well-tolerated at physiological concentrations. Standard laboratory safety practices should be followed when handling this research peptide.
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| References | |
| Additional Infomation |
Leptin (93-105) is a research-use peptide and is not approved for therapeutic use. It is a synthetic fragment derived from the human leptin sequence and is used primarily in metabolic research. The peptide is supplied as a lyophilized powder and should be stored frozen. It is soluble in DMSO at 50 mg/mL.
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| Molecular Formula |
C64H110N20O23
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|---|---|
| Molecular Weight |
1527.68000
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| Exact Mass |
1526.81
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| CAS # |
200436-43-7
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| PubChem CID |
102214966
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0.926
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| Hydrogen Bond Donor Count |
23
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| Hydrogen Bond Acceptor Count |
25
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| Rotatable Bond Count |
53
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| Heavy Atom Count |
107
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| Complexity |
3190
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| Defined Atom Stereocenter Count |
15
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| SMILES |
CC[C@@H]([C@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N)CC(N)=O)=O)C(C)C)=O)[C@H](CC)C)=O)CCC(N)=O)=O)C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(O)=O)CCCNC(N)=N)=O)CC(C)C)=O)CC(N)=O)=O)CCC(O)=O)=O)CC(C)C)=O)CC(O)=O)=O)CC(N)=O)=O)CO)=O)C
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| InChi Key |
OFDBWUQCFOBTMQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C64H110N20O23/c1-11-30(9)49(83-53(96)33(15-17-42(66)86)74-61(104)50(31(10)12-2)84-60(103)48(29(7)8)82-51(94)32(65)22-43(67)87)62(105)81-41(26-85)59(102)79-39(24-45(69)89)57(100)80-40(25-47(92)93)58(101)77-36(20-27(3)4)54(97)73-34(16-18-46(90)91)52(95)78-38(23-44(68)88)56(99)76-37(21-28(5)6)55(98)75-35(63(106)107)14-13-19-72-64(70)71/h27-41,48-50,85H,11-26,65H2,1-10H3,(H2,66,86)(H2,67,87)(H2,68,88)(H2,69,89)(H,73,97)(H,74,104)(H,75,98)(H,76,99)(H,77,101)(H,78,95)(H,79,102)(H,80,100)(H,81,105)(H,82,94)(H,83,96)(H,84,103)(H,90,91)(H,92,93)(H,106,107)(H4,70,71,72)
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| Chemical Name |
4-[[2-[[2-[[4-amino-2-[[2-[[2-[[5-amino-2-[[2-[[2-[(2,4-diamino-4-oxobutanoyl)amino]-3-methylbutanoyl]amino]-3-methylpentanoyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]amino]-3-hydroxypropanoyl]amino]-4-oxobutanoyl]amino]-3-carboxypropanoyl]amino]-4-methylpentanoyl]amino]-5-[[4-amino-1-[[1-[[1-carboxy-4-(diaminomethylideneamino)butyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-oxopentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~32.73 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6546 mL | 3.2729 mL | 6.5459 mL | |
| 5 mM | 0.1309 mL | 0.6546 mL | 1.3092 mL | |
| 10 mM | 0.0655 mL | 0.3273 mL | 0.6546 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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