| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
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| Targets |
Lepidiline A targets the enzyme HSD17B1 (17β-hydroxysteroid dehydrogenase type 1). It inhibits HSD17B1 activity, increasing the bioconversion efficiency of sex hormones. This enzyme is involved in steroid metabolism. Its cytotoxic activity is against HL-60 leukemia cells.
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| ln Vitro |
Lepidiline A shows cytotoxic activity against HL-60 cells with an IC50 of 32.3 μM. It inhibits HSD17B1 activity. It has antimicrobial, antioxidant, and anti-inflammatory effects. Its activity is confirmed in cell-based and enzymatic assays.
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| ln Vivo |
Lepidiline A improves the balance of endogenous sex hormones in mice and increases the fecundity of fruit flies. It has been studied for its potential in modulating cellular signaling pathways and protecting against oxidative stress. Its in vivo effects are related to its HSD17B1 inhibition.
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| Enzyme Assay |
The primary assay for Lepidiline A's mechanism is a biochemical assay measuring its inhibition of HSD17B1 activity. The enzyme is incubated with its substrate, and the production of the product (e.g., estrogen from estrone) is measured in the presence of the compound.
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| Cell Assay |
In vitro cellular assays for Lepidiline A involve treating cancer cell lines, such as HL-60, to assess its cytotoxic activity. Its anti-inflammatory activity can be assessed in immune cells by measuring the production of inflammatory cytokines. Its effect on HSD17B1 can be studied in cell-based assays.
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| Animal Protocol |
In vivo studies for Lepidiline A have been conducted in mice to assess its effects on sex hormone balance. Its effects on fecundity have been studied in fruit flies. Other in vivo models for inflammation and cancer would be relevant for studying its biological activities.
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| ADME/Pharmacokinetics |
Detailed pharmacokinetic data for Lepidiline A is not provided. It is a natural product with a molecular weight of 312.84 g/mol. Its properties as a natural compound are being characterized. Its suitability for in vivo use is indicated by its effects in animal models.
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| Toxicity/Toxicokinetics |
Toxicological data for Lepidiline A is not detailed. As a natural product and research compound, its safety profile is not fully established. It is not an approved drug. Standard laboratory safety practices should be followed.
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| References | |
| Additional Infomation |
See also: Lepidium meyenii root (part).
Lepidiline A is a natural alkaloid from Maca with a unique imidazole structure. It has shown promising biological activities, including cytotoxicity, anti-inflammation, and modulation of steroid metabolism. Its activity against HSD17B1 makes it a potential lead compound for research in endocrinology and oncology. |
| Molecular Formula |
C19H21CLN2
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|---|---|
| Molecular Weight |
312.84
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| Exact Mass |
314.155
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| CAS # |
596093-98-0
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| PubChem CID |
404702
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| Appearance |
White to off-white solid powder
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| LogP |
4.891
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
22
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| Complexity |
275
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZATWGHMQWRXUNZ-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C19H21N2.ClH/c1-16-17(2)21(14-19-11-7-4-8-12-19)15-20(16)13-18-9-5-3-6-10-18;/h3-12,15H,13-14H2,1-2H3;1H/q+1;/p-1
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| Chemical Name |
1,3-dibenzyl-4,5-dimethylimidazol-1-ium;chloride
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| Synonyms |
Macaline A; Lepidiline A
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~199.78 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1965 mL | 15.9826 mL | 31.9652 mL | |
| 5 mM | 0.6393 mL | 3.1965 mL | 6.3930 mL | |
| 10 mM | 0.3197 mL | 1.5983 mL | 3.1965 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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