| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
LD 490 does not have a specific biological target in the traditional pharmacological sense. As a laser dye, its primary interaction is with light, absorbing at specific wavelengths and emitting fluorescence. In biological contexts, coumarin derivatives may interact with various enzymes or receptors depending on their structure. Coumarin 6H has been shown to have anti-inflammatory properties, suggesting potential interactions with inflammatory mediators, though the specific molecular target is not well-characterized.
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| ln Vitro |
When inhibited by a fluorescence quenching group Fluorescence, coumarin 6H with a twisted block and a stronger electronic julolidine group demonstrates higher quantum yield, better biocompatibility, and less background. Consequently, LH-1's coumarin 6H may aid in lowering background articulation. In LH-1, 2,4-dinitroacetyl can also quench the pronunciation of the coumarin 6H pronunciation group by using the donor-excited photoinductive electron transfer (d-PET) mechanism, in addition to the NN group's rotation. quenching of groups [2].
In vitro, LD 490 exhibits fluorescent properties characteristic of coumarin dyes, with absorption and emission spectra suitable for laser applications. The compound's fluorescence emission can be enhanced through hydrogen bonding interactions. Coumarin 6H has been shown to have anti-inflammatory properties in cellular assays. Detailed in vitro activity data including specific IC50 or EC50 values for biological effects are limited in the published literature. |
| ln Vivo |
In vivo, LD 490 has been studied for its anti-inflammatory properties. As a coumarin derivative, it may exhibit biological activities similar to other coumarins, including anti-inflammatory, antioxidant, and antimicrobial effects. However, specific in vivo data for LD 490 are limited, as the compound is primarily used as a fluorescent probe or laser dye rather than as a therapeutic agent.
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| Enzyme Assay |
In vitro binding assays for LD 490 are not typically performed as the compound is primarily used as a laser dye rather than a pharmacological agent. However, fluorescence spectroscopy is used to characterize the compound's photophysical properties. Absorption and emission spectra are measured using a spectrophotometer and spectrofluorometer, respectively. The quantum yield and fluorescence lifetime may also be determined. For biological applications, cell uptake and fluorescence microscopy studies may be performed.
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| Cell Assay |
Cellular assays for LD 490 typically involve fluorescence microscopy or flow cytometry to assess cellular uptake and intracellular localization. Cells are incubated with the compound, and fluorescence is measured using appropriate excitation and emission wavelengths. Cell viability assays (e.g., MTT or CCK-8) may be performed to assess cytotoxicity. Anti-inflammatory activity may be assessed by measuring cytokine production in activated immune cells.
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| Animal Protocol |
In vivo animal studies for anti-inflammatory activity would typically use rodent models of inflammation such as carrageenan-induced paw edema or LPS-induced cytokine production. Animals would be administered LD 490 orally or intraperitoneally, and the inflammatory response would be assessed by measuring paw swelling, cytokine levels, or other inflammatory markers. However, specific in vivo protocols for LD 490 are limited in the published literature.
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| ADME/Pharmacokinetics |
LD 490 is a small-molecule coumarin derivative with physicochemical properties typical of fluorescent dyes. It is expected to have good solubility in organic solvents and limited aqueous solubility. The compound's PK properties would depend on the route of administration and formulation. As a research compound, detailed PK parameters are not extensively published. The compound is primarily used in laboratory settings rather than as a therapeutic agent.
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| Toxicity/Toxicokinetics |
LD 490 has been shown to have anti-inflammatory properties and is generally considered to have low toxicity. Standard toxicity assessments would include acute toxicity in rodents and cytotoxicity assays in cell lines. The compound is primarily used as a research tool, and comprehensive toxicity data are not extensively published. Coumarin derivatives are generally considered to have favorable safety profiles, though some coumarins have hepatotoxic potential at high doses.
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| References | |
| Additional Infomation |
LD 490 (Coumarin 6H, C-6H) is a coumarin derivative that functions as a laser dye. The fluorescence emission of Coumarin 6H can be enhanced through hydrogen bonding interactions. The compound has also been shown to have anti-inflammatory properties. LD 490 is used primarily as a research tool in fluorescence spectroscopy and microscopy applications. It is available for research use only and is not approved for therapeutic applications.
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| Molecular Formula |
C15H15NO2
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|---|---|
| Molecular Weight |
241.29
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| Exact Mass |
241.11
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| CAS # |
58336-35-9
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| PubChem CID |
94022
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| Appearance |
Brown to breen solid powder
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| Density |
1.32g/cm3
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| Boiling Point |
486.3ºC at 760 mmHg
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| Melting Point |
130 °C
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| Flash Point |
201ºC
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| Index of Refraction |
1.665
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| LogP |
2.556
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
18
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| Complexity |
392
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MZSOXGPKUOAXNY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H15NO2/c17-13-6-5-11-9-10-3-1-7-16-8-2-4-12(14(10)16)15(11)18-13/h5-6,9H,1-4,7-8H2
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| Chemical Name |
3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one
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| Synonyms |
LD490; LD-490; LD 490
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~51.80 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1444 mL | 20.7220 mL | 41.4439 mL | |
| 5 mM | 0.8289 mL | 4.1444 mL | 8.2888 mL | |
| 10 mM | 0.4144 mL | 2.0722 mL | 4.1444 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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