| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg | |||
| Other Sizes |
| Targets |
Ladanein targets the hepatitis C virus (HCV) by disrupting the interactions of core and other HCV proteins. It is a phytochemicals inhibitor that interferes with HCV replication. As a flavonoid, ladanein may also interact with multiple molecular targets involved in inflammation and oxidative stress, such as enzymes and signaling pathways like NF-κB, MAPK, and PI3K/Akt. It can also act as an antioxidant by scavenging free radicals and chelating metal ions.
|
|---|---|
| ln Vitro |
In vitro, ladanein is an effective anti-HCV agent. It is a phytochemicals inhibitor that is known to disrupt the interactions of core and other hepatitis C virus (HCV) proteins. As a flavonoid, it is expected to exhibit antioxidant and anti-inflammatory activities. However, specific IC50 values for its antiviral or other activities are not detailed in the available literature. In cell-based assays, ladanein can inhibit HCV replication in infected cells.
|
| ln Vivo |
In vivo, ladanein has been studied for its potential antiviral effects against HCV. However, specific in vivo data are not detailed in the available literature. As a flavonoid, it may have limited oral bioavailability due to its sugar moiety, but it could be hydrolyzed to its aglycone form in the gastrointestinal tract. Further studies are needed to determine its pharmacokinetic and pharmacodynamic properties in animal models.
|
| Enzyme Assay |
Non-cellular in vitro assays for ladanein involve studying its antiviral activity against HCV. A typical protocol uses an HCV replicon system or a cell-free assay to measure the inhibition of HCV protein interactions or replication. The compound is incubated with HCV proteins or RNA, and the inhibition of replication or protein-protein interactions is measured. Alternatively, its antioxidant activity can be measured using cell-free assays such as DPPH radical scavenging or ABTS assays, where the compound is incubated with the radical solution and the decrease in absorbance is measured spectrophotometrically.
|
| Cell Assay |
Cellular assays for ladanein are performed using HCV-infected cell lines or hepatocytes. Cells are treated with ladanein at various concentrations for 24-72 hours. HCV replication is measured by quantifying HCV RNA by RT-PCR or by measuring HCV core protein levels by ELISA. Cell viability is measured using an MTT assay to ensure that the observed effects are not due to cytotoxicity. The IC50 for inhibition of HCV replication is determined.
|
| Animal Protocol |
In vivo animal studies for ladanein are conducted in mouse models of HCV infection, such as humanized mouse models or transgenic mice expressing HCV proteins. Animals are administered ladanein orally or intraperitoneally at doses such as 10-50 mg/kg. Viral load is measured in blood and liver tissues. Liver histology is examined to assess the effects on inflammation and fibrosis. However, specific published protocols for ladanein are limited in the available literature.
|
| ADME/Pharmacokinetics |
Ladanein has a molecular formula of 5,6-dihydroxy-7,4'-dimethoxyflavone and is soluble in chloroform, dichloromethane, ethyl acetate, DMSO, and acetone. It is a solid powder that should be stored desiccated at -20°C. Its molecular weight is not specified in the available literature. Detailed pharmacokinetic parameters have not been reported.
|
| Toxicity/Toxicokinetics |
Detailed toxicological data for ladanein have not been extensively reported. As a natural product used for research, it is generally considered to have low toxicity, but comprehensive safety data are not available. Standard laboratory safety precautions should be followed when handling ladanein. It is not a drug and is not intended for human use.
|
| References | |
| Additional Infomation |
Latanine is a dimethoxyflavonoid, a derivative of baicalin, with its 4' and 7' hydroxyl groups replaced by methoxy groups. It is a potent antiviral drug against hepatitis C virus (HCV). Latanine has applications as a plant metabolite, antiviral agent, and free radical scavenger. It is a dimethoxyflavonoid and a dihydroxyflavonoid, functionally related to baicalin. Latanine has been reported in bees (Apis), peppermint (Nepeta pungens), and several other organisms with relevant data.
Ladanein is a naturally occurring flavonoid compound found in various plants, particularly in the genus Ladanum and isolated from Thymus piperella. It is a dimethoxyflavone (5,6-dihydroxy-7,4'-dimethoxyflavone) that is an effective anti-HCV agent. It disrupts the interactions of core and other hepatitis C virus (HCV) proteins. Ladanein has also gained attention for its antioxidant, anti-inflammatory, and potential anticancer properties. It is not a clinically approved drug and has not entered clinical trials. Its primary value is as a research tool for natural product chemistry and antiviral research. |
| Molecular Formula |
C17H14O6
|
|---|---|
| Molecular Weight |
314.28946
|
| Exact Mass |
314.079
|
| CAS # |
10176-71-3
|
| Related CAS # |
10176-71-3;
|
| PubChem CID |
3084066
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
565.7±50.0 °C at 760 mmHg
|
| Melting Point |
310-313℃
|
| Flash Point |
212.4±23.6 °C
|
| Vapour Pressure |
0.0±1.6 mmHg at 25°C
|
| Index of Refraction |
1.646
|
| LogP |
3.49
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
23
|
| Complexity |
468
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
UUQJTIHOVGMQIH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C17H14O6/c1-21-10-5-3-9(4-6-10)12-7-11(18)15-13(23-12)8-14(22-2)16(19)17(15)20/h3-8,19-20H,1-2H3
|
| Chemical Name |
5,6-dihydroxy-7-methoxy-2-(4-methoxyphenyl)chromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1818 mL | 15.9089 mL | 31.8177 mL | |
| 5 mM | 0.6364 mL | 3.1818 mL | 6.3635 mL | |
| 10 mM | 0.3182 mL | 1.5909 mL | 3.1818 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
