| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
||
| 10g |
|
||
| 25g |
|
||
| 50g |
|
||
| Other Sizes |
| Targets |
Lactamide does not have a specific pharmacological target. It functions as a cryoprotectant by stabilizing cells during freezing and as a hyperosmotic agent in experimental settings.
|
|---|---|
| ln Vitro |
In vitro, Lactamide has been used as a cryoprotectant for the cryopreservation of white rabbit sperm. It is also used as a 2M solution for ciliary epithelium in experimental models.
|
| ln Vivo |
In vivo, Lactamide's primary use is as a cryoprotectant in experimental settings. No significant pharmacological effects have been reported. It is used in research to study cryobiology and osmotic stress.
|
| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for Lactamide as it does not have a known specific enzyme or receptor target.
|
| Cell Assay |
In vitro cellular assays for Lactamide involve testing its effectiveness as a cryoprotectant. Cells or tissues (e.g., sperm cells) are suspended in a medium containing the compound and subjected to a freeze-thaw cycle. Post-thaw cell viability, motility (for sperm), or membrane integrity is assessed to evaluate the protective effect of Lactamide compared to standard cryoprotectants like glycerol.
|
| Animal Protocol |
In vivo animal experiments using Lactamide are limited to its use in cryopreservation studies, where it may be used to preserve tissues or cells for transplantation or research. No specific animal model of disease is associated with Lactamide.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for Lactamide are not relevant for its primary use as a cryoprotectant. It is typically used ex vivo or in vitro. When used systemically, it is expected to be metabolized to lactic acid. No specific PK profile is available in the literature.
|
| Toxicity/Toxicokinetics |
Lactamide is considered an irritant, with GHS classifications indicating it causes skin and serious eye irritation. It is not known to be systemically toxic at the concentrations used for cryopreservation. It has been evaluated for safety in cosmetic applications.
|
| Additional Infomation |
Lactamide is a monocarboxylic acid amide, formed when the carboxylic acid group of lactic acid is converted into the corresponding carboxylamide group. It is both a monocarboxylic acid amide and a secondary alcohol. Functionally, it is related to racemic lactic acid.
Lactamide is a simple amide compound used primarily as a research reagent and cryoprotectant. It is not a drug and has no approved therapeutic indications. It is also known as NSC-61454 and lactic acid amide. Its use in research is focused on cryobiology and as a chemical intermediate. |
| Molecular Formula |
C3H7NO2
|
|---|---|
| Molecular Weight |
89.09
|
| Exact Mass |
89.047
|
| CAS # |
2043-43-8
|
| PubChem CID |
94220
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.161g/cm3
|
| Boiling Point |
281.9ºC at 760 mmHg
|
| Melting Point |
73-76 °C(lit.)
|
| Flash Point |
124.3ºC
|
| Vapour Pressure |
0.000409mmHg at 25°C
|
| Index of Refraction |
1.459
|
| LogP |
-1.1
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
6
|
| Complexity |
61.8
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O([H])C([H])(C(N([H])[H])=O)C([H])([H])[H]
|
| InChi Key |
SXQFCVDSOLSHOQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C3H7NO2/c1-2(5)3(4)6/h2,5H,1H3,(H2,4,6)
|
| Chemical Name |
2-hydroxypropanamide
|
| Synonyms |
NSC-61454 NSC 61454 Lactamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 11.2246 mL | 56.1230 mL | 112.2460 mL | |
| 5 mM | 2.2449 mL | 11.2246 mL | 22.4492 mL | |
| 10 mM | 1.1225 mL | 5.6123 mL | 11.2246 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
