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| Targets |
L-Methionylglycine serves as a substrate for peptidases and is used to study peptide hydrolysis and amino acid transport. The compound does not have a specific therapeutic target but is used as a research tool to understand peptide metabolism. It is hydrolyzed by enzymes such as dipeptidases to release L-methionine and glycine, which are essential amino acids involved in protein synthesis, methylation reactions, and neurotransmission.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro, L-Methionylglycine is used as a substrate to study the activity of dipeptidases and other peptidases. The rate of hydrolysis of the dipeptide can be measured by detecting the release of free amino acids using HPLC, amino acid analyzers, or colorimetric assays. The compound is also used in cell culture studies to assess peptide transport mechanisms, including the Peptide Transporter 1 (PEPT1) and other oligopeptide transporters. |
| ln Vivo |
In vivo, L-Methionylglycine is used in nutritional studies to assess amino acid absorption and metabolism. It can be administered orally or intravenously, and the appearance of methionine and glycine in the blood is measured over time. This allows researchers to study the efficiency of peptide absorption and the role of peptide transporters in the gastrointestinal tract. Detailed in vivo activity data are not extensively reported in the available literature.
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| Enzyme Assay |
Non-cell-based enzyme assays for L-Methionylglycine involve incubating the dipeptide with a sample containing dipeptidase activity (e.g., purified enzyme, tissue homogenate, or intestinal brush border membrane vesicles) in a suitable buffer. The reaction is incubated at 37°C for a specified time, and the reaction is stopped. The released amino acids (methionine and glycine) are quantified using HPLC, amino acid analyzers, or colorimetric assays. Enzyme activity is calculated from the rate of amino acid release.
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| Cell Assay |
Cellular assays for L-Methionylglycine utilize cell lines expressing peptide transporters (e.g., PEPT1-expressing cells) to study peptide uptake. Cells are incubated with the dipeptide at various concentrations and times, and intracellular or extracellular amino acid levels are measured. The compound's transport kinetics (Km, Vmax) can be determined. Inhibition studies with other peptides or transporter inhibitors can be performed to characterize the transport mechanism.
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| Animal Protocol |
In vivo animal models for L-Methionylglycine involve administering the dipeptide to animals (e.g., rats, mice) via oral gavage or intravenous injection. Blood samples are collected at various time points, and plasma amino acid levels are measured. The appearance of methionine and glycine in the blood is used to assess peptide absorption and hydrolysis. Intestinal perfusion studies may also be performed to study peptide transport across the intestinal epithelium.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-Methionylglycine include CAS number 14486-03-4. The compound has a molecular formula of C7H14N2O3S. It is a small dipeptide that is soluble in water. Detailed PK parameters such as half-life and bioavailability are not extensively reported, as the compound is primarily used as a research tool rather than a therapeutic agent.
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| Toxicity/Toxicokinetics |
L-Methionylglycine is a dipeptide with generally low toxicity. Detailed toxicological data are not extensively reported, as the compound is not intended for therapeutic use. It is composed of two amino acids that are naturally occurring and essential for human health. Standard laboratory safety precautions should be followed when handling the compound. It is for research use only.
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| References | |
| Additional Infomation |
Met-Gly is a dipeptide formed from L-methionine and glycine residues. It is a metabolite that is functionally related to L-methionine and glycine.
L-Methionylglycine is a dipeptide composed of L-methionine and glycine. It has CAS number 14486-03-4. It is used as a research tool for studying peptide transport, metabolism, and enzymatic hydrolysis. It is hydrolyzed by dipeptidases to release free methionine and glycine. It is used in nutritional research and cell culture studies. It is for research use only. |
| Molecular Formula |
C7H14N2O3S
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| Molecular Weight |
206.26
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| Exact Mass |
206.073
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| CAS # |
14486-03-4
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| PubChem CID |
6992930
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| Appearance |
White to off-white solid powder
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| Density |
1.267 g/cm3
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| Boiling Point |
487.4ºC at 760 mmHg
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| Melting Point |
212ºC
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| Flash Point |
248.6ºC
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| Vapour Pressure |
7.78E-11mmHg at 25°C
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| Index of Refraction |
1.541
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| LogP |
0.358
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
13
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| Complexity |
187
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| Defined Atom Stereocenter Count |
1
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| SMILES |
S(C([H])([H])[H])C([H])([H])C([H])([H])[C@@]([H])(C(N([H])C([H])([H])C(=O)O[H])=O)N([H])[H]
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| InChi Key |
QXOHLNCNYLGICT-YFKPBYRVSA-N
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| InChi Code |
InChI=1S/C7H14N2O3S/c1-13-3-2-5(8)7(12)9-4-6(10)11/h5H,2-4,8H2,1H3,(H,9,12)(H,10,11)/t5-/m0/s1
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| Chemical Name |
2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]acetic acid
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| Synonyms |
NSC 88865; Met-Gly; L-Methionylglycine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8482 mL | 24.2412 mL | 48.4825 mL | |
| 5 mM | 0.9697 mL | 4.8482 mL | 9.6965 mL | |
| 10 mM | 0.4848 mL | 2.4241 mL | 4.8482 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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