| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
JOE does not have a specific biological target as it is a fluorescent dye used for labeling rather than a pharmacologically active compound. Its function is to provide fluorescence signal for the detection and quantification of nucleic acids in various analytical applications. The dye is used as a fluorescent label in DNA sequencing, PCR, and ligase chain reaction (LCR) amplifications. JOE's fluorescence properties allow for precise monitoring of nucleic acid interactions and concentrations.
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| ln Vitro |
In vitro, JOE is used as a fluorescent label for nucleic acid detection in various molecular biology applications. It exhibits an absorbance maximum at 525 nm and an emission maximum at 550 nm, providing enhanced sensitivity and specificity for detecting DNA. The dye is employed in DNA sequencing, PCR, and LCR amplifications as a fluorescent reporter. Its high quantum yield and low pH sensitivity make it suitable for quantitative fluorescence-based assays in the physiological pH range.
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| ln Vivo |
JOE is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a research tool for in vitro nucleic acid detection and analysis. The compound is used in laboratory settings for DNA sequencing and PCR applications. It is not administered to animals for therapeutic purposes but rather used ex vivo for analytical applications.
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| Enzyme Assay |
In vitro experiments with JOE typically involve preparing stock solutions in DMSO or appropriate buffers and diluting them to working concentrations for labeling reactions. For nucleic acid labeling, the dye is conjugated to oligonucleotide probes or primers through amine or other reactive groups. The labeled nucleic acids are then used in PCR, sequencing, or hybridization assays. Fluorescence is measured using a spectrophotometer or plate reader with excitation at approximately 525 nm and emission at 550 nm. The dye's fluorescence is stable under typical assay conditions.
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| Cell Assay |
In vitro cell-based assays using JOE are not typical as the dye is primarily used for nucleic acid detection in cell-free systems. However, JOE-labeled probes can be used in fluorescence in situ hybridization (FISH) or other cell-based nucleic acid detection applications. Cells are fixed and hybridized with JOE-labeled probes, and fluorescence is visualized using microscopy with appropriate excitation and emission filters. The dye's low pH sensitivity ensures reliable signal detection under various experimental conditions.
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| Animal Protocol |
JOE is not typically used for in vivo animal experiments. The dye is primarily employed in in vitro analytical applications such as DNA sequencing and PCR. For potential in vivo imaging applications, the dye would need to be conjugated to targeting molecules and formulated for administration, but such applications are not standard for this compound.
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| ADME/Pharmacokinetics |
JOE has a molecular weight of 505.26 g/mol and the formula C23H14Cl2O9. The compound is a solid and is typically stored at -20°C, protected from light. Its fluorescence properties include an absorbance maximum at approximately 525 nm and an emission maximum at approximately 550 nm. JOE is soluble in DMSO and other organic solvents. The dye is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Specific toxicological data for JOE are not extensively provided in the available sources. As a fluorescent dye, it is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The dye may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References | |
| Additional Infomation |
6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein is a fluorescein compound with chlorine substituents at the 4' and 5' positions, methoxy substituents at the 2' and 7' positions, and a carboxyl substituent at the 6' position. It is a fluorescent dye. Its function is related to fluorescein (lactone form).
JOE (CAS 82855-40-1) is also known as 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein. It is a conventional fluorophore predominantly used in automated DNA sequencing. The dye exhibits an absorbance maximum at 525 nm and an emission maximum at 550 nm, with high quantum yield and low pH sensitivity. JOE's emission is red-shifted compared to carboxyfluorescein (FAM), allowing for multiplex assays. The compound is used as a fluorescent label in DNA sequencing, PCR, and LCR amplifications. It is intended for research use only. |
| Molecular Formula |
C23H14CL2O9
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|---|---|
| Molecular Weight |
505.257865428925
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| Exact Mass |
504.001
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| CAS # |
82855-40-1
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| PubChem CID |
10229054
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| Appearance |
Brown to reddish brown solid powder
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| LogP |
4.688
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
34
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| Complexity |
790
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IDLISIVVYLGCKO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C23H14Cl2O9/c1-31-13-6-11-19(15(24)17(13)26)33-20-12(7-14(32-2)18(27)16(20)25)23(11)10-5-8(21(28)29)3-4-9(10)22(30)34-23/h3-7,26-27H,1-2H3,(H,28,29)
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| Chemical Name |
4',5'-dichloro-3',6'-dihydroxy-2',7'-dimethoxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9792 mL | 9.8959 mL | 19.7918 mL | |
| 5 mM | 0.3958 mL | 1.9792 mL | 3.9584 mL | |
| 10 mM | 0.1979 mL | 0.9896 mL | 1.9792 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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