| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
Jaranol does not have a single defined pharmacological target. As a flavonoid, it likely interacts with multiple cellular targets to exert its biological activities. Jaranol has been shown to induce apoptosis in ovarian cancer cells, suggesting involvement in pro-apoptotic signaling pathways. The compound may modulate cell cycle regulation, inhibit proliferation, and activate cell death pathways. As a kaempferol derivative, it may share some of the biological activities of kaempferol, including antioxidant, anti-inflammatory, and anti-cancer properties. However, the specific molecular targets have not been fully characterized.
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| ln Vitro |
In vitro, Jaranol inhibits the growth of SKOV3 ovarian cancer cells. It induces apoptosis in ovarian cancer cells, demonstrating anti-cancer activity. As a flavonoid, Jaranol would be expected to exhibit antioxidant activity through free radical scavenging and metal chelation. However, specific IC50 values or detailed activity data is not provided in the available sources. The compound's diverse biological activities make it a valuable tool for studying cancer biology and natural product pharmacology.
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| ln Vivo |
In vivo, Jaranol has been studied for its potential therapeutic applications. As an apoptosis-inducing compound, it would be expected to have anti-tumor activity in animal models. However, specific in vivo efficacy data from animal models is not detailed in the available sources. The compound's natural occurrence in cloves and other plants suggests it may have a favorable safety profile at dietary levels. Comprehensive in vivo studies would be required for therapeutic development.
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| Enzyme Assay |
For apoptosis-inducing compounds, standard cell-free assays include caspase activity assays using fluorogenic substrates, and assessment of mitochondrial membrane potential using fluorescent dyes. For antioxidant activity, standard cell-free assays include DPPH radical scavenging, ABTS radical cation decolorization, and ferric reducing antioxidant power (FRAP) assays.
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| Cell Assay |
For apoptosis-inducing compounds, standard cellular assays involve treatment of cancer cell lines (e.g., SKOV3 ovarian cancer cells) with the test compound for 24-72 hours. Apoptosis is assessed by Annexin V/PI staining, caspase activity assays, and DNA fragmentation. Cell viability is measured using MTT or CellTiter-Glo assays to determine IC50 values. Cell cycle analysis is performed by flow cytometry.
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| Animal Protocol |
For in vivo evaluation of anti-cancer compounds, standard animal models include tumor xenograft models using cancer cell lines sensitive to the compound. The compound is typically administered orally or intraperitoneally for 2-4 weeks. Tumor growth, apoptosis markers, and survival are assessed. For Jaranol, specific in vivo protocols have not been detailed in the available sources.
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| ADME/Pharmacokinetics |
Detailed pharmacokinetic data for Jaranol is not provided in the available sources. The compound has a molecular weight of 314.29 and formula C17H14O6. As a flavonoid with moderate lipophilicity, it would be expected to have reasonable oral absorption. Comprehensive pharmacokinetic studies would be required for therapeutic development.
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| Toxicity/Toxicokinetics |
Detailed toxicology data for Jaranol is not provided in the available sources. As a naturally occurring flavonoid found in cloves and other plants, it may have a favorable safety profile at moderate doses. However, comprehensive toxicological evaluation would be required for therapeutic development. The compound is for research use only and not for therapeutic applications.
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| References | |
| Additional Infomation |
Kumatakenin is a flavonoid compound belonging to the ether class of compounds. It has been reported to exist in Melicope semecarpifolia, Osyris alba, and other organisms with relevant data. See also: Glycyrrhiza glabra (partial); Glycyrrhiza uralensis Root (partial).
Jaranol is also known as Kumatakenin, Kaempferol 3,7-dimethyl ether, 3,7-Di-O-methylkaempferol, and 4',5-dihydroxy-3,7-dimethoxyflavone. It has the UNII code 5FAQ11412T. The compound is a natural flavonoid extracted from cloves that induces apoptosis in ovarian cancer cells. No regulatory approval has been identified. |
| Molecular Formula |
C17H14O6
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|---|---|
| Molecular Weight |
314.29
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| Exact Mass |
314.079
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| CAS # |
3301-49-3
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| PubChem CID |
5318869
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| Appearance |
Light yellow to green yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
578.4±50.0 °C at 760 mmHg
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| Flash Point |
217.4±23.6 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.671
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| LogP |
2.79
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
23
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| Complexity |
480
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BJBUTJQYZDYRMJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O6/c1-21-11-7-12(19)14-13(8-11)23-16(17(22-2)15(14)20)9-3-5-10(18)6-4-9/h3-8,18-19H,1-2H3
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| Chemical Name |
5-hydroxy-2-(4-hydroxyphenyl)-3,7-dimethoxychromen-4-one
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| Synonyms |
Kamatakenin; Kumatakillin; Jaranol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1818 mL | 15.9089 mL | 31.8177 mL | |
| 5 mM | 0.6364 mL | 3.1818 mL | 6.3635 mL | |
| 10 mM | 0.3182 mL | 1.5909 mL | 3.1818 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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