| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Isovanillic acid targets oxidative stress pathways and exhibits antioxidant activity through its phenolic hydroxyl group, which can scavenge free radicals. As a phenolic acid, it may also modulate inflammatory signaling pathways and interact with enzymes involved in phenolic acid metabolism.
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|---|---|
| ln Vitro |
Isovanillic acid exhibits antioxidant activity through its ability to scavenge free radicals and reduce oxidative stress. It may also have anti-inflammatory properties. The compound is used as a research tool for studying the biological activities of phenolic acids and their potential health benefits.
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| ln Vivo |
Isovanillic acid is a metabolite of isovanillin that is excreted in urine. It is formed through the metabolism of dietary phenolic compounds and may contribute to the antioxidant and anti-inflammatory effects of phenolic acid-rich foods. The compound's levels in biological fluids reflect dietary intake and metabolism of phenolic compounds.
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| Enzyme Assay |
In vitro non-cell assays for Isovanillic acid typically involve antioxidant activity measurements using DPPH, ABTS, or FRAP assays. The compound is incubated with the radical-generating system, and the decrease in absorbance is measured spectrophotometrically to calculate the scavenging activity and IC₅₀ values.
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| Cell Assay |
In vitro cell-based assays for Isovanillic acid use cell lines to study its effects on oxidative stress and inflammation. Cells are treated with the compound and exposed to oxidative insults or inflammatory stimuli. Parameters such as reactive oxygen species levels, antioxidant enzyme activities, and inflammatory cytokine production are measured.
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| Animal Protocol |
In vivo animal studies for Isovanillic acid may involve oral administration of the compound to rodents to study its absorption, metabolism, and biological effects. Parameters assessed include plasma and tissue levels of the compound and its metabolites, oxidative stress markers, and inflammatory markers.
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| ADME/Pharmacokinetics |
Isovanillic acid has a molecular weight of 168.15 g/mol and a molecular formula of C₈H₈O₄. It is soluble in water and organic solvents. The compound is a natural phenolic acid found in various plants. Detailed pharmacokinetic parameters have not been extensively characterized.
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| Toxicity/Toxicokinetics |
Isovanillic acid is generally considered to have low toxicity as a natural phenolic compound. Standard laboratory safety precautions should be followed when handling the compound. The compound is classified as a research reagent and is not intended for human therapeutic use.
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| References | |
| Additional Infomation |
3-Hydroxy-4-methoxybenzoic acid is a methoxybenzoic acid derivative formed by introducing a hydroxyl substituent at the 3-position of 4-methoxybenzoic acid. It possesses antibacterial activity and is also a plant metabolite. It is both a methoxybenzoic acid and a monohydroxybenzoic acid. It is the conjugate acid of 3-hydroxy-4-methoxybenzoic acid ester. 3-Hydroxy-4-methoxybenzoic acid has been reported to exist in Hypericum erectum, Euphorbia polycaulis, and other organisms with relevant data.
Isovanillic acid is a phenolic acid and a metabolite of isovanillin. It is an isomer of vanillic acid and is used as a research reagent in studies of phenolic acid metabolism and antioxidant activity. Not approved for therapeutic use; intended for research purposes only. |
| Molecular Formula |
C8H8O4
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|---|---|
| Molecular Weight |
168.1467
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| Exact Mass |
168.042
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| CAS # |
645-08-9
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| Related CAS # |
Isovanillic acid; 645-08-9
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| PubChem CID |
12575
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
344.3±27.0 °C at 760 mmHg
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| Melting Point |
250-253 °C(lit.)
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| Flash Point |
145.2±17.2 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.586
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| Source |
Originated from plants: Scrophulariaceae Scrophularia ningpoensis Hemsl.
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| LogP |
1.35
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
12
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| Complexity |
168
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(O)C1=CC=C(OC)C(O)=C1
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| InChi Key |
LBKFGYZQBSGRHY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H8O4/c1-12-7-3-2-5(8(10)11)4-6(7)9/h2-4,9H,1H3,(H,10,11)
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| Chemical Name |
3-hydroxy-4-methoxybenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~594.71 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (14.87 mM) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Suspended solution; with ultrasonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (14.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9471 mL | 29.7354 mL | 59.4707 mL | |
| 5 mM | 1.1894 mL | 5.9471 mL | 11.8941 mL | |
| 10 mM | 0.5947 mL | 2.9735 mL | 5.9471 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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