| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Indo-1 AM targets intracellular calcium ions (Ca2+). Upon binding to Ca2+, the dye exhibits a shift in its emission spectrum, enabling ratiometric quantification. The AM ester moiety facilitates cell permeability; the dye itself is a Ca2+ chelator with a dissociation constant (Kd) of approximately 250 nM for Ca2+.
|
|---|---|
| ln Vitro |
1. Working solution preparation for Indo-1 AM 1.1 Stock solution preparation: Extract Indo-1 AM and mix it with anhydrous DMSO to make a 10 mM stock solution. Note: It is advised to aliquot and store the Indo-1 AM storage solution at -20 °C or -80 °F. 1.2 Features of the working solution Make a 1–10 μM Indo-1 AM working solution using the pre-made HBSS storage solution. Note: 2. Please modify the Indo-1 AM working solution's concentration in accordance with the current circumstances. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. One ×106/mL is the density. 2.2 Pour in 1 milliliter of the Indo-1 AM working solution. 2.3 Centrifuge for 3–4 minutes at 400 g, discarding the supernatant. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Use flow cytometry or fluorescence once the cells have been resuspended in 1 mL HBSS 3. Adherent cell staining: 3.1 Grow the adherent cells on sterile coverslips. 3.2 Take off the coverslip from the cells and use a little aspirator to extract a few cells. 3.3 Add the dye working solution in 100 μL. 3.4 Aspirate the dye working solution, rinse with culture medium two or three times for five minutes each, and use a fluorescence microscope to view.
In vitro, Indo-1 AM serves as a fluorescent Ca2+ indicator with excitation at 340 nm and dual emission at 405 nm (Ca2+-bound) and 485 nm (Ca2+-free). In rabbit hearts loaded with Indo-1 AM, 72% of the dye localizes in the cytosol, with the remainder associated with mitochondria, microsomes, and the nucleus. |
| ln Vivo |
In vivo activity data for Indo-1 AM are not applicable as the compound is a fluorescent probe used for ex vivo or in vitro cellular calcium measurements, not a therapeutic agent. It is employed in physiological studies to monitor intracellular calcium fluctuations in isolated tissues, including perfused hearts.
|
| Enzyme Assay |
In vitro enzyme/receptor binding assays are not performed for Indo-1 AM, as it is a fluorescent probe rather than a pharmacological agent targeting enzymes or receptors. The compound's mechanism involves calcium chelation and fluorescence emission change upon binding, which is characterized by spectrophotometric rather than binding assay methods.
|
| Cell Assay |
Cellular assays for Indo-1 AM involve loading cells with the dye (typically 1-5 uM) in serum-free or low-serum medium for 30-60 minutes at 37degC. After washing to remove extracellular dye, cells are analyzed by flow cytometry or fluorescence microscopy using UV excitation (340-355 nm). Emission is collected at 405 nm (Ca2+-bound) and 485 nm (Ca2+-free) for ratiometric calculation of intracellular Ca2+ concentration.
|
| Animal Protocol |
In vivo animal protocols for Indo-1 AM are not applicable. The compound is used exclusively as a research reagent for cellular calcium imaging in isolated cells, tissue slices, or perfused organs. It is not administered to animals for pharmacological evaluation.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Indo-1 AM are not characterized, as the compound is a research reagent rather than a therapeutic drug. The AM ester is hydrolyzed intracellularly to the active Indo-1 form, which is membrane-impermeant and trapped inside cells. The compound is typically stored as a powder at -20degC and protected from light.
|
| Toxicity/Toxicokinetics |
Toxicological data for Indo-1 AM are not extensively characterized. As a fluorescent probe used in cell-based assays at low micromolar concentrations, it is generally considered non-toxic to cells during typical experimental timeframes. Standard laboratory safety precautions apply. The compound is for research use only.
|
| References |
|
| Additional Infomation |
Indo-1 AM is a widely used research tool for measuring intracellular calcium dynamics in various cell types. It is commercially available as a high-purity reagent for flow cytometry, fluorescence microscopy, and plate-reader-based calcium flux assays. Unlike Fura-2, Indo-1 exhibits dual emission properties, making it suitable for ratiometric measurements with a single excitation wavelength. It is for research use only and not for human therapeutic or diagnostic applications.
|
| Molecular Formula |
C47H51N3O22
|
|---|---|
| Molecular Weight |
1009.91474
|
| Exact Mass |
1009.3
|
| CAS # |
112926-02-0
|
| PubChem CID |
123918
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.374g/cm3
|
| Boiling Point |
1009.2ºC at 760mmHg
|
| Flash Point |
564.1ºC
|
| Vapour Pressure |
3.2E-28mmHg at 25°C
|
| Index of Refraction |
1.577
|
| LogP |
3.146
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
24
|
| Rotatable Bond Count |
37
|
| Heavy Atom Count |
72
|
| Complexity |
1800
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
CAWBRCOBJNWRLK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3
|
| Chemical Name |
acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9902 mL | 4.9509 mL | 9.9018 mL | |
| 5 mM | 0.1980 mL | 0.9902 mL | 1.9804 mL | |
| 10 mM | 0.0990 mL | 0.4951 mL | 0.9902 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
