| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| 25g |
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| Other Sizes |
| Targets |
Indigotindisulfonate sodium does not have a specific pharmacological target. It is used as a diagnostic dye and indicator. It is administered intravenously for renal function testing, where it is excreted by the kidneys and its appearance in the urine is monitored.
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|---|---|
| ln Vitro |
Indigo carmine diluted in water to a concentration of 0.2% turns blue at pH 11.4 and yellow at pH 13.0. Another redox indicator that appears yellow when reduced is indigo carmine [1].
Indigotindisulfonate sodium is not a pharmacologically active compound in the context of drug discovery. It is used as a diagnostic agent and a redox and pH indicator. Its activity is related to its color and solubility properties. |
| ln Vivo |
Indigotindisulfonate sodium is used clinically as a diagnostic agent for renal function testing. It is administered intravenously, and its excretion by the kidneys is monitored. It is not used for therapeutic purposes.
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| Enzyme Assay |
Indigotindisulfonate sodium is not a drug and does not have a defined in vitro assay protocol for enzyme or receptor binding. It is a dye and diagnostic agent. Its purity and identity are verified using analytical techniques such as HPLC and UV-Vis spectroscopy.
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| Cell Assay |
Indigotindisulfonate sodium is not used in cell-based assays for pharmacological activity. It may be used as a stain in certain histological or cytological applications, but it does not have a defined cellular target.
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| Animal Protocol |
Indigotindisulfonate sodium is used in vivo as a diagnostic agent. It is administered intravenously, and its appearance in the urine is monitored to assess renal function. This is a standard clinical procedure.
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| ADME/Pharmacokinetics |
Indigotindisulfonate sodium is administered intravenously for diagnostic purposes. It is rapidly excreted by the kidneys. Its half-life is short, and it is cleared from the body primarily through renal excretion.
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| Toxicity/Toxicokinetics |
Indigotindisulfonate sodium is generally considered safe at the doses used for diagnostic purposes. It can cause allergic reactions in some individuals. It is not used at high doses for therapeutic purposes.
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| References | |
| Additional Infomation |
Indigo to dark blue powder. (NTP, 1992)
Indigo carmine is an organosodium salt formed by the condensation of indigo carmine (acid form) with two equivalents of sodium hydroxide. It acts as an indicator at pH 11.5-14, changing color from blue to yellow. It can be used as a food coloring agent, histological dye, and bicolor indicator. It contains indigo carmine (2-). Indolesulfonic acid can be used as a dye for nitrates and chlorates in renal function tests, and also in milk testing. See also: Sodium indigo disulfonate (note moved to). Indigotindisulfonate sodium is a diagnostic agent used for renal function testing. It is also used as a food colorant and a redox and pH indicator. It is not a therapeutic agent. |
| Molecular Formula |
C16H8N2NA2O8S2
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|---|---|
| Molecular Weight |
466.35
|
| Exact Mass |
465.951
|
| CAS # |
860-22-0
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| PubChem CID |
2723854
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| Appearance |
Brown to black solid powder
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| Density |
1.01 g/mL at 20 °C
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| Melting Point |
>300°C
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| Index of Refraction |
n20/D 1.335
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| LogP |
3.06
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
30
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| Complexity |
877
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JFVXEJADITYJHK-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17,19H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2
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| Chemical Name |
disodium;2-(3-hydroxy-5-sulfonato-1H-indol-2-yl)-3-oxoindole-5-sulfonate
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| Synonyms |
NSC-8646 NSC 8646 Indigotindisulfonate sodium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~18.52 mg/mL (~39.71 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1443 mL | 10.7216 mL | 21.4431 mL | |
| 5 mM | 0.4289 mL | 2.1443 mL | 4.2886 mL | |
| 10 mM | 0.2144 mL | 1.0722 mL | 2.1443 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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