| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
Heptasaccharide Glc4Xyl3 targets xyloglucan-active enzymes, specifically endo-xyloglucanases, which hydrolyze xyloglucan polysaccharides. Xyloglucan is a major hemicellulose component of plant cell walls and is involved in cell wall structure and growth. Endo-xyloglucanases cleave the beta-1,4-glucan backbone of xyloglucan, releasing oligosaccharide products including heptasaccharides like Glc4Xyl3. As a covalent/irreversible inhibitor, Heptasaccharide Glc4Xyl3 forms a stable complex with the enzyme active site, preventing substrate hydrolysis. This makes it a valuable tool for identifying and characterizing xyloglucan-active enzymes in nature.
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| ln Vitro |
In vitro, Heptasaccharide Glc4Xyl3 acts as a covalent/irreversible inhibitor of endo-xyloglucanases. The compound binds to the active site of the enzyme, forming a stable covalent adduct that prevents the hydrolysis of xyloglucan substrates. This inhibition allows researchers to identify and analyze multiple xyloglucan-active enzymes in complex mixtures. The heptasaccharide is also used as a substrate for studying the catalytic mechanism and substrate specificity of glycoside hydrolases. In enzyme kinetics assays, Heptasaccharide Glc4Xyl3 can be used as a substrate to measure enzyme activity and determine kinetic parameters.
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| ln Vivo |
In vivo, Heptasaccharide Glc4Xyl3 is used as a research tool to study xyloglucan metabolism and plant cell wall biology. The compound is not a therapeutic agent but rather a biochemical tool for studying enzyme activities in plant and microbial systems. As an inhibitor of endo-xyloglucanases, Heptasaccharide Glc4Xyl3 can be used to probe the role of these enzymes in plant growth, development, and stress responses. However, specific in vivo data for this compound are limited in publicly available sources. The compound is intended for research use only.
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| Enzyme Assay |
In vitro enzyme assays for Heptasaccharide Glc4Xyl3 involve measuring endo-xyloglucanase activity using xyloglucan or synthetic substrates. The enzyme is incubated with Heptasaccharide Glc4Xyl3 as a substrate or inhibitor in assay buffer at 37degC for 30-60 minutes. For substrate assays, the release of reducing sugars is measured using the Nelson-Somogyi or DNS method. For inhibition assays, the compound is pre-incubated with the enzyme before adding the xyloglucan substrate, and residual activity is measured. Inhibition constants (IC₅0, Ki) are determined from dose-response curves. Product analysis is performed by HPLC or mass spectrometry to identify the cleavage products.
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| Cell Assay |
In vitro cellular assays for Heptasaccharide Glc4Xyl3 are performed using plant cells or tissues to study xyloglucan metabolism. Plant cell cultures (e.g., Arabidopsis, tobacco) are treated with Heptasaccharide Glc4Xyl3 at concentrations of 0.1-100 microM for 1-24 hours. Xyloglucan degradation is assessed by measuring the release of oligosaccharides into the culture medium using HPLC or mass spectrometry. Gene expression of xyloglucan-active enzymes is quantified by qRT-PCR. Cell wall composition is analyzed by monosaccharide analysis. The effects of the compound on plant growth, cell elongation, and stress responses are evaluated. However, specific protocols are not well-documented in publicly available sources.
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| Animal Protocol |
In vivo animal studies for Heptasaccharide Glc4Xyl3 are not typically performed, as the compound is used as a research tool for studying plant carbohydrate metabolism rather than as a therapeutic agent. The compound is not intended for administration to animals or humans. Researchers interested in in vivo applications should consult the primary literature for the most current information. The compound is a biochemical reagent for laboratory use only.
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| ADME/Pharmacokinetics |
Heptasaccharide Glc4Xyl3 (CAS#: 121591-98-8) has molecular formula C3₉H₆₆O33 and molecular weight 1,062.92. It is a xyloglucan heptasaccharide also known as XXXG, consisting of four glucose (Glc) and three xylose (Xyl) residues. The compound is a covalent/irreversible inhibitor of endo-xyloglucanases and is used for the identification and analysis of xyloglucan-active enzymes in nature. Purity is typically >95.0% by HPLC. The compound is a solid and should be stored at room temperature. It is soluble in water and aqueous buffers. The compound is intended for research use only.
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| References | |
| Additional Infomation |
Heptasaccharide Glc4Xyl3 (CAS#: 121591-98-8), also known as XXXG or Xyloglucan Heptasaccharide Glc4Xyl3, is a carbohydrate molecule consisting of four glucose (Glc) and three xylose (Xyl) residues. It is used as a substrate or model compound for studying enzyme activities and carbohydrate-binding proteins in glycobiology and carbohydrate chemistry. The compound is a covalent/irreversible inhibitor of endo-xyloglucanases and is used to identify and analyze multiple xyloglucan-active enzymes in nature. It is supplied as a research chemical for laboratory use only. As of the current date, Heptasaccharide Glc4Xyl3 is not approved for any clinical or therapeutic indication.
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| Molecular Formula |
C39H66O33
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|---|---|
| Molecular Weight |
1062.9216
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| Exact Mass |
1062.35
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| CAS # |
121591-98-8
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| Appearance |
Typically exists as solid at room temperature
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| Index of Refraction |
1.701
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| SMILES |
O(C1([H])C([H])(C([H])(C([H])(C([H])(C([H])([H])OC2([H])C([H])(C([H])(C([H])(C([H])([H])O2)O[H])O[H])O[H])O1)OC1([H])C([H])(C([H])(C([H])(C([H])(C([H])([H])OC2([H])C([H])(C([H])(C([H])(C([H])([H])O2)O[H])O[H])O[H])O1)O[H])O[H])O[H])O[H])O[H])C1([H])C([H])(C([H])([H])OC2([H])C([H])(C([H])(C([H])(C([H])([H])O2)O[H])O[H])O[H])OC([H])(C([H])(C1([H])O[H])O[H])OC1([H])C([H])(C([H])([H])O[H])OC([H])(C([H])(C1([H])O[H])O[H])O[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9408 mL | 4.7040 mL | 9.4080 mL | |
| 5 mM | 0.1882 mL | 0.9408 mL | 1.8816 mL | |
| 10 mM | 0.0941 mL | 0.4704 mL | 0.9408 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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