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| Targets |
HEPBS does not have a specific pharmacological target as it is a biological buffer. Its function is to maintain a stable pH in biological and biochemical systems. As a zwitterionic buffer, it can act as both a proton donor and acceptor, resisting changes in pH when acids or bases are added. Its higher pKa makes it useful for applications requiring pH control in the alkaline range (7.6-9.0). HEPBS has very low metal ion binding affinity, minimizing interference with metal-dependent biological processes.
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| ln Vitro |
In vitro, HEPBS is used as a buffering agent in various biochemical and cell biology applications. It is used in cell culture and other cell experiments. The buffer's pH range of 7.6-9.0 makes it suitable for applications requiring alkaline pH conditions. HEPBS has very low metal ion binding affinity, making it useful for studies involving metal ions. The buffer is compatible with most biological systems and does not interfere with enzymatic reactions or cell growth.
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| ln Vivo |
HEPBS is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a laboratory buffer for in vitro experiments and cell culture applications. The compound is used in the preparation of solutions for biological experiments. Its role is limited to maintaining pH homeostasis in experimental systems. HEPBS is not administered to animals for therapeutic purposes.
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| Enzyme Assay |
In vitro experiments using HEPBS involve preparing buffer solutions at the desired pH (range 7.6-9.0) by dissolving the appropriate amount of HEPBS in deionized water. The buffer is typically used at concentrations of 10-50 mM. For cell culture applications, HEPBS is added to culture media at 10-25 mM to maintain pH. For enzyme assays, HEPBS buffer is used to prepare substrate solutions and reaction mixtures. The buffer's low metal ion binding affinity makes it suitable for metalloenzyme studies. The pH of the buffer solution can be adjusted with acid or base as needed.
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| Cell Assay |
In vitro cell-based assays using HEPBS involve its incorporation into cell culture media to maintain pH. The compound is dissolved in cell culture medium at concentrations of 10-25 mM. Cells are cultured in HEPBS-buffered media under standard conditions. The buffer is compatible with most cell types and does not affect cell viability at standard concentrations. For studies requiring precise pH control in the alkaline range (7.6-9.0), HEPBS provides effective buffering. The buffer's low metal ion binding affinity minimizes interference with metal-dependent cellular processes.
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| Animal Protocol |
In vivo animal experiments using HEPBS are not typical as the compound is a research buffer rather than a therapeutic agent. However, HEPBS-buffered solutions may be used in animal studies for the administration of other compounds where pH control is critical. When used in animal studies, HEPBS would be formulated as a buffered saline solution for injection or oral administration. The compound's primary role is to maintain pH stability in administered solutions rather than to exert any pharmacological effect.
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| ADME/Pharmacokinetics |
HEPBS has a molecular weight of 266.36 g/mol and the formula C10H22N2O4S. The compound is a zwitterionic buffer with a pH range of 7.6-9.0. It has very low metal ion binding affinity. For storage, the powder is kept at room temperature in a dry place. The compound appears as a white powder. Its purity is typically ≥99%. HEPBS is a homolog of HEPES and EPPS with a higher pKa value. The buffer is stable under normal storage conditions.
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| Toxicity/Toxicokinetics |
HEPBS is generally considered to have low toxicity as a biological buffer. The compound is not intended for human or veterinary use and is classified for research purposes only. Safety data sheets indicate that standard laboratory handling precautions should be followed, including the use of appropriate personal protective equipment. The compound is stable under normal storage conditions. Acute toxicity data are limited, but as a zwitterionic buffer, it is not expected to be highly toxic. In cell culture applications, HEPBS is used at concentrations that are compatible with cell viability and growth.
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| References | |
| Additional Infomation |
HEPBS (CAS 161308-36-7) is a zwitterionic biological buffer belonging to the Good's buffer family. It is also known as N-(2-hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) and has the molecular formula C10H22N2O4S. HEPBS has a pH range of 7.6-9.0 and is useful in biological systems. The buffer contains a piperazine ring and a butanesulfonic acid moiety, which contribute to its buffering capacity and solubility. HEPBS has very low metal ion binding affinity. It is used for cell culture and other cell experiments. The compound is intended for research use only.
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| Molecular Formula |
C10H22N2O4S
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| Molecular Weight |
266.35768
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| Exact Mass |
266.13
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| CAS # |
161308-36-7
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| PubChem CID |
9965191
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Index of Refraction |
1.532
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| LogP |
-2.16
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
17
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| Complexity |
294
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
LOJNFONOHINEFI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H22N2O4S/c13-9-8-12-6-4-11(5-7-12)3-1-2-10-17(14,15)16/h13H,1-10H2,(H,14,15,16)
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| Chemical Name |
4-[4-(2-hydroxyethyl)piperazin-1-yl]butane-1-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~469.29 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7543 mL | 18.7716 mL | 37.5432 mL | |
| 5 mM | 0.7509 mL | 3.7543 mL | 7.5086 mL | |
| 10 mM | 0.3754 mL | 1.8772 mL | 3.7543 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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