| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| Other Sizes |
|
| ln Vitro |
In vitro oocyte growth and development are inhibited by halofenozide (RH-0345) [1].
|
|---|---|
| ln Vivo |
Following the topical administration of halobenzohydrazide (RH-0345; 5 and 10 μg) to mature females, the maximum dosage (10 μg) notably decreased scores for three reproductive parameters: fecundity, egg viability, and oviposition period [1]. Eggs exposed to halofenyl hydrazide at two tested concentrations (5 and 10 μg) showed reduced length, breadth, and volume [1].
|
| Cell Assay |
Cell viability assay [2]
Cell Types: ovaries dissected and cultured from adult female mealworms, T. molitor Tested Concentrations: 1 μM and 10 μM Incubation Duration: 4 days Experimental Results: No significant effect on the amount of ecdysteroids The effects were in culture medium using 0-day-old and 2-day-old female ovaries at a concentration of 10 μM. A concentration of 1 μM in culture medium and 2-day-old ovaries caused a small significant increase in ovarian ecdysteroid content in the culture medium. |
| Animal Protocol |
Animal/Disease Models: Emerging (0 days old) adult female yellow stork [1]
Doses: 5 and 10 μg Route of Administration: Topical application Experimental Results: Resulted in smaller eggs in length, width and volume at both test doses diminished (5 and 10 μg). |
| Toxicity/Toxicokinetics |
Non-Human Toxicity Values
LC50 Rat inhalation >2.7 mg/L/4 hr LD50 Rabbit dermal >2000 mg/kg LD50 Mouse oral 2214 mg/kg LD50 Rat oral 2850 mg/kg |
| References |
[1]. Faïza Taïbi, et al. Effect of ecdysone agonist RH-0345 on reproduction of mealworm, Tenebrio molitor. Comp Biochem Physiol C Toxicol Pharmacol. 2003 Jul;135C(3):257-67.
|
| Additional Infomation |
Halofenozide is a bisacylhydrazine insecticide and a member of monochlorobenzenes. It is functionally related to a N'-benzoyl-N-(tert-butyl)benzohydrazide.
Mechanism of Action Ecdysone agonist causing premature molting; insect growth regulator. |
| Molecular Formula |
C18H19CLN2O2
|
|---|---|
| Molecular Weight |
330.81
|
| Exact Mass |
330.114
|
| CAS # |
112226-61-6
|
| PubChem CID |
114994
|
| Appearance |
White solid
|
| Density |
1.205g/cm3
|
| Melting Point |
198.0-199.0°
|
| Index of Refraction |
1.584
|
| LogP |
4.316
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
23
|
| Complexity |
419
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(C)(C)N(C(=O)C1=CC=CC=C1)NC(=O)C2=CC=C(C=C2)Cl
|
| InChi Key |
CNKHSLKYRMDDNQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C18H19ClN2O2/c1-18(2,3)21(17(23)14-7-5-4-6-8-14)20-16(22)13-9-11-15(19)12-10-13/h4-12H,1-3H3,(H,20,22)
|
| Chemical Name |
N'-benzoyl-N'-tert-butyl-4-chlorobenzohydrazide
|
| Synonyms |
RH-0345; RH 0345; Halofenozide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~377.86 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0229 mL | 15.1144 mL | 30.2288 mL | |
| 5 mM | 0.6046 mL | 3.0229 mL | 6.0458 mL | |
| 10 mM | 0.3023 mL | 1.5114 mL | 3.0229 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
