| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
H-β-HoAla-OH.HCl is an alanine analogue used in biochemical research. It targets enzyme-substrate interactions and catalytic mechanisms. The extended carbon chain of β-homoamino acids can affect how enzymes recognize and process the amino acid, making it valuable for studying enzyme specificity. It is also used in peptide synthesis as a building block for incorporating β-amino acids into peptide sequences.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro studies with H-β-HoAla-OH.HCl focus on its use as a research tool. Amino acids and amino acid derivatives affect the release of anabolic hormones and fuel availability. As a β-amino acid, it can be used to study enzyme-substrate interactions and catalytic mechanisms. The compound can be incorporated into peptides to study the effects of backbone extension on peptide conformation and biological activity. It is a valuable tool in protein engineering. |
| ln Vivo |
There is no specific in vivo activity data for H-β-HoAla-OH.HCl. β-Amino acids and their derivatives are generally not naturally occurring and are used primarily as research tools. Peptides containing β-homoalanine residues may be evaluated in vivo for various applications, including the development of metabolically stable peptide therapeutics. Animal studies would be performed on the final peptide products containing β-amino acid residues.
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| Enzyme Assay |
In vitro enzyme/receptor binding protocols for H-β-HoAla-OH.HCl involve studying its interactions with enzymes. The compound is incubated with purified enzymes in appropriate buffers at physiological pH. Enzyme activity is measured using spectrophotometric or HPLC methods. For substrate specificity studies, the compound is tested as a potential substrate or inhibitor. Binding affinity can be determined using isothermal titration calorimetry or surface plasmon resonance. The hydrochloride salt form facilitates dissolution in aqueous buffers.
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| Cell Assay |
Cell culture protocols for H-β-HoAla-OH.HCl involve dissolving the compound in sterile water or cell culture medium. Cells are treated with concentrations ranging from 0.1-100 mM for 24-72 hours. Effects on cell viability, proliferation, and metabolism are assessed using standard assays such as MTT or resazurin reduction. The compound can be used to study amino acid transport and metabolism in cell models. The hydrochloride form provides good aqueous solubility for cell culture applications.
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| Animal Protocol |
In vivo animal study protocols for H-β-HoAla-OH.HCl are not well established as it is primarily a research tool. If used in animal studies, the compound would be dissolved in saline or PBS and administered via injection or oral gavage. Dosing would be determined empirically. Blood and tissue samples would be collected for PK analysis. Studies would focus on the effects of β-amino acid incorporation on metabolism and physiology.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of H-β-HoAla-OH.HCl are characteristic of small amino acid derivatives. The compound has a molecular weight of 139.58 g/mol and formula C₄H₁₀ClNO₂. The hydrochloride salt form provides good aqueous solubility. As a non-natural amino acid, it may be metabolized differently from natural amino acids. Elimination is expected via renal excretion. The compound shows purity of ≥98%.
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| Toxicity/Toxicokinetics |
Limited toxicity data is available for H-β-HoAla-OH.HCl. Standard safety precautions for handling amino acid derivatives should be observed. The compound is not intended for human therapeutic use. Acute toxicity is expected to be low. Store as powder at -20°C. Proper personal protective equipment should be worn when handling. No specific toxicity studies have been reported. It is for research use only.
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| References | |
| Additional Infomation |
H-β-HoAla-OH.HCl (CAS#: 58610-41-6) is also known as L-β-homoalanine hydrochloride, (S)-3-aminobutyric acid hydrochloride, and H-β-HoAla-OH·HCl. Its molecular formula is C₄H₉NO₂·HCl and molecular weight is 139.58. It has a purity of ≥98% by NMR and appears as a white powder. It is a non-proteinogenic amino acid used in enzyme-substrate interaction studies. It has no approved therapeutic indications.
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| Molecular Formula |
C4H10CLNO2
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| Molecular Weight |
139.5807
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| Exact Mass |
215.071
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| CAS # |
58610-41-6
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| PubChem CID |
2761505
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| Appearance |
White to off-white powder
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| Boiling Point |
223.6ºC at 760mmHg
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| Flash Point |
89ºC
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| LogP |
1.31
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
8
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| Complexity |
72.1
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| Defined Atom Stereocenter Count |
1
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| SMILES |
Cl[H].O([H])C(C([H])([H])[C@]([H])(C([H])([H])[H])N([H])[H])=O
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| InChi Key |
UHYVVUABAWKTJJ-DFWYDOINSA-
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| InChi Code |
InChI=1S/C4H9NO2.ClH/c1-3(5)2-4(6)7;/h3H,2,5H2,1H3,(H,6,7);1H/t3-;/m0./s1
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| Chemical Name |
(3S)-3-aminobutanoic acid;hydrochloride
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| Synonyms |
L-beta-Homoalanine hydrochloride; H-β-HoAla-OH.HCl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.1644 mL | 35.8218 mL | 71.6435 mL | |
| 5 mM | 1.4329 mL | 7.1644 mL | 14.3287 mL | |
| 10 mM | 0.7164 mL | 3.5822 mL | 7.1644 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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