| Size | Price | Stock | Qty |
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| 1g |
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| Other Sizes |
| Targets |
As a protected aspartic acid derivative, H-Asp(OtBu)-OH does not have a specific biological target. Its primary utility is as a chemical building block in organic synthesis, particularly in peptide chemistry. The compound serves as a protected aspartic acid unit that can be incorporated into peptide chains while the tert-butyl ester protects the side-chain carboxyl group. Aspartic acid is a non-essential amino acid, but in its protected form, the compound is not designed to interact with biological receptors.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
H-Asp(OtBu)-OH does not exhibit pharmacological activity in vitro. As a protected amino acid derivative, it is a synthetic intermediate rather than a bioactive compound. In vitro studies using this compound focus on its chemical reactivity in peptide bond formation reactions. The compound may be used as a substrate in enzymatic assays to study esterase activity. Its role in research is almost exclusively as a reagent for organic synthesis. |
| ln Vivo |
H-Asp(OtBu)-OH is not a pharmacologically active compound and therefore does not have defined in vivo activity as a drug. When administered to animals, the compound would likely be metabolized to release aspartic acid. However, the compound is not used therapeutically, and its in vivo effects would be limited to those of the released aspartic acid. Its primary value remains in synthetic chemistry.
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| Enzyme Assay |
In vitro assays for H-Asp(OtBu)-OH focus on its chemical properties in peptide synthesis. The compound is typically used as a building block in solid-phase or solution-phase peptide synthesis. It is dissolved in polar aprotic solvents and coupled to other amino acids using standard peptide coupling reagents. The tert-butyl ester can be selectively removed under acidic conditions, enabling orthogonal deprotection strategies.
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| Cell Assay |
In vitro cellular assays using H-Asp(OtBu)-OH are limited due to the compound's role as a synthetic intermediate. However, it can be used in cell culture studies to investigate the intracellular delivery of aspartic acid. Cells are cultured in media supplemented with the compound, and cellular uptake and aspartic acid release are monitored. The effects of increased intracellular aspartic acid on cellular metabolism can be assessed in cell lines such as hepatocytes or neurons.
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| Animal Protocol |
In vivo animal studies with H-Asp(OtBu)-OH are not typically conducted, as the compound is a synthetic intermediate rather than a pharmacologically active agent. If used in vivo, the compound would be administered to animals to study the metabolism of protected amino acid derivatives. However, such studies are rare, and the compound is generally used exclusively in synthetic chemistry applications.
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| ADME/Pharmacokinetics |
H-Asp(OtBu)-OH is not a drug candidate, and pharmacokinetic data are not available. As a protected amino acid derivative, it is designed for chemical synthesis rather than systemic administration. If administered in vivo, the compound would likely be hydrolyzed to release aspartic acid. The compound's pharmacokinetic properties have not been characterized, and its use is confined to in vitro synthetic applications.
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| Toxicity/Toxicokinetics |
The compound is generally considered to have low toxicity, consistent with its use as a chemical reagent. The compound should be stored away from moisture. Standard laboratory safety precautions are recommended.
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| References |
[1]. Luckose F, et al. Effects of amino acid derivatives on physical, mental, and physiological activities. Crit Rev Food Sci Nutr. 2015;55(13):1793-1144.
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| Additional Infomation |
H-Asp(OtBu)-OH (L-Aspartic acid 4-tert-butyl ester, CAS 3057-74-7) is a protected amino acid derivative used as a building block in peptide synthesis. Its chemical formula is C8H15NO4 and molecular weight is 189.21. The compound features a tert-butyl ester protecting the side-chain carboxyl group. It is intended for research use only.
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| Molecular Formula |
C8H15NO4
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|---|---|
| Molecular Weight |
189.209
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| Exact Mass |
189.1
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| CAS # |
3057-74-7
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| PubChem CID |
7010570
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
318.7±37.0 °C at 760 mmHg
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| Melting Point |
220°C (dec.)
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| Flash Point |
146.5±26.5 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.476
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| LogP |
0.94
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
207
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| Defined Atom Stereocenter Count |
1
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| SMILES |
O=C(O)[C@@H](N)CC(OC(C)(C)C)=O
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| InChi Key |
MXWMFBYWXMXRPD-YFKPBYRVSA-N
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| InChi Code |
InChI=1S/C8H15NO4/c1-8(2,3)13-6(10)4-5(9)7(11)12/h5H,4,9H2,1-3H3,(H,11,12)/t5-/m0/s1
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| Chemical Name |
(2S)-2-amino-4-[(2-methylpropan-2-yl)oxy]-4-oxobutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2851 mL | 26.4257 mL | 52.8513 mL | |
| 5 mM | 1.0570 mL | 5.2851 mL | 10.5703 mL | |
| 10 mM | 0.5285 mL | 2.6426 mL | 5.2851 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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